Effect of liquid culture requirements on antifungal antibiotic production by Streptomyces rimosus MY02

Jia, Yu; Qiu, Liu; Qiao, Li; Liu, Xiangdong; Sun, Qiang; Yan, Jin; Qi, Xiaohui; Sheng-di, Fan

doi:10.1016/j.biortech.2007.03.023

Cited by 59 publications

(44 citation statements)

References 4 publications

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“…The present results were in agreement with previous studies [43,44] which reported the optimum for antibiotic production from Streptomyces species; Streptomyces hygroscopic was pH 7 or near neutral environments. Another study said that the optimum pH for the fermentation of antibiotic, granaticin by Streptomyces thermoviolaceus was 6.5-7.0 and the Streptomyces isolate failed to grow at acidic pH but grow well at pH 7-8 [45].…”

Section: Initial Ph Of Production Mediumsupporting

confidence: 94%

Optimization Production Conditions of Antibacterial Metabolite From Streptomyces Sp.

Al-Ghazali

Omran

2017

Asian J Pharm Clin Res

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Objectives: The paper aimed to isolate Streptomyces strain having the ability to produce antibacterial metabolites and optimize some environmental parameters for excellent antibiotic production.Methods: Different soil samples were collected from extreme environments of desert regions at Karbala Province, Iraq. Actinomycetes were isolated using different media. The primary screening for antibacterial production was accomplished, and the antibacterial activities were tested against pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, and Pseudomonas aeruginosa. The most potent strain was chosen for optimizing some of environmental parameters to increase the bioactive metabolite production. Different parameters were studied such as culture media, temperature, pH, and agitation rate.Results: About eight Streptomyces strains were isolated from soil samples. All isolates appeared variable levels of antibiotic productions against Grampositive and negative pathogenic bacteria, and the best one was Streptomyces sp. LHR 9. The antibacterial metabolite production from Streptomyces sp. LHR 9 was affected by various cultural parameters. Glucose soybean meal broth as a fermentation medium at pH 7 yielded the highest antibiotic production under the optimal fermentation conditions, including the temperature at 35°C with 200 rpm (revolution/min) agitation rate and 7 days incubation period. Conclusion:The Streptomyces sp. LHR 9 showed antibacterial activity against both Gram-positive and negative pathogenic bacteria. It may consider as a potential source of drug production. Further study needs to purification and characterization of antibiotic and analyzes the mechanism for the antimicrobial activity of this bioactive compound.

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Section: Initial Ph Of Production Mediumsupporting

confidence: 94%

Optimization Production Conditions of Antibacterial Metabolite From Streptomyces Sp.

Al-Ghazali

Omran

2017

Asian J Pharm Clin Res

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“…Production of antibiotic metabolite has been known to be influenced by media components and cultural conditions, such as aeration, agitation, pH, temperature and glycerol concentration, which vary from organism to organism (Iwai and Omura, 1982;Yu et al, 2008). Also environmental factors like incubation temperature, pH and incubation time were found to have profound influence on antibiotic production (Srinivasan et al, 1991;VenkataDasu and Panda, 1999;Sujathaet al, 2005).…”

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confidence: 99%

Production of Antibacterial and AntifungalMetabolites by (S.albovinaceus) Strain no.10/2 and MediaOptimization

Abdelghani¹

2017

AIJB

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A new strain ofActinomycete (S. albovinaceus) no.10/2 with a broad antifungal and antibacterial activitywas isolated from soil sample of Palestine.The medium composition for antibioticproduction fromstrain S.albovinaceus was optimized using shake-flask methodology.The parameters that resulted in maximum antibiotic production are: 1.25 % mannitol and 1 % malt extract were found to be best carbon and nitrogen sources respectively for growth and antifungal and antibacterial production. Similarly initial pH of 7.2, 10 % level of inoculum, incubation period of 96 hrs, and agitation in 250 ml bottom indented flask and medium to flask ratio of 1:10 (aeration) were found to be optimal.Optimization of medium and cultural conditions resulted in good increase inantifungaland antibacterial yield.This is one of the first reports for strain no.10/2 (S. albovinaceus) reported from Palestine soil with antibacterial and antifungal activities.

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“…Arasu et al (2009) found that, the maximum antibiotic produced by Streptomyces sp. ER1-3 was obtained at pH 7.0 while Yu et al (2008) found that the best pH for antifungal antibiotic production by Streptomyces rimosus MY02 was 6.0.…”

Section: Saccharomonospora Viridismentioning

confidence: 98%

Production, Structural Elucidation and Biological Activities of Bioactive Metabolite of MarineSaccharomonospora viridi

2016

Egypt. J. Bot.

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wenty seven of one hundred thirty five marine actinomycete …….isolates were producers. Analysis of the 16S rRNA gene of the most potent bioactive isolate showed a strong similarity with Saccharomonospora viridis AHK190. For the production of antimicrobial metabolites, nutritional and environmental conditions have been optimized. Maximum antibiotic production was obtained when galactose was used as a sole carbon source at a concentration of 4.5% (w/v) in the presence of 7% (w/v) aspartic acid with salinity level 3% (w/v) and K 2 HPO 4 at 0.011%. Similarly initial medium pH of 8.0, incubation temperature of 55°C and incubation period of 72 hr were found to be the optimum. The chemical structure of this compound was elucidated on the basis of its spectroscopy data, elemental analysis and its molecular formula was found to be C 31 H 34 O 4 S 3 . The minimum inhibitory concentration of the pure compound against the tested bacteria was found to be in the range of 125-250 μg/ml and the minimum bacteriocidal concentration of the purified antibiotic was ranged between 250 and 500 μg/ml. For fungi, minimum inhibitory concentration was ranged between250-500µg/ml. The pure compound also found to have cytotoxic activity against human colon carcinoma with lethal concentration 50 of 24.2µg/ml.

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Effect of liquid culture requirements on antifungal antibiotic production by Streptomyces rimosus MY02

Cited by 59 publications

References 4 publications

Optimization Production Conditions of Antibacterial Metabolite From Streptomyces Sp.

Optimization Production Conditions of Antibacterial Metabolite From Streptomyces Sp.

Production of Antibacterial and AntifungalMetabolites by (S.albovinaceus) Strain no.10/2 and MediaOptimization

Production, Structural Elucidation and Biological Activities of Bioactive Metabolite of MarineSaccharomonospora viridi

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