Effect of lipopolysaccharide on cell proliferation and vascular endothelial growth factor secretion of periodontal ligament stem cells

Keong, Jia Yee; Low, Li Wei; Chong, Jean Mun; Ong, Yan Yi; Pulikkotil, Shaju Jacob; Singh, Gaurav Pratap; Nagendrababu, Venkateshbabu; Banavar, Spoorthi Ravi; Khoo, Suan Phaik

doi:10.1016/j.sdentj.2019.08.001

Cited by 14 publications

(10 citation statements)

References 27 publications

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“…These effects are associated with disease pathogenic mechanisms. Cell proliferation of PDLSCs was reduced in a dose-independent manner to E. coli-LPS [89], in contrast to previous findings where cell proliferation remained stable over time [90]. This study suggests that PDLSCs may remain viable with time and have a possible adaptive mechanism to continuous exposure to bacterial LPS.…”

Section: Microbial Effects On Other Functions Of Stem Cellscontrasting

confidence: 96%

“…This endotoxin tolerance indicates that PDLSCs retain their regenerative potential even at sites with remaining periodontal inflammation. Within the study limitations [89], the secretion of VEGF, which is associated with periodontal regeneration [91], decreased independent of the LPS concentration at both 48 and 96 h, but significantly increased between the two time-points, as PDLSCs remained viable to secret this factor over time. In line with previous work, prolonged exposure to E.…”

Section: Microbial–stem Cell Interactionsmentioning

confidence: 99%

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Microbial–stem cell interactions in periodontal disease

Iliopoulos

Layrolle

Apatzidou

2022

Journal of Medical Microbiology

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Periodontitis is initiated by hyper-inflammatory responses in the periodontal tissues that generate dysbiotic ecological changes within the microbial communities. As a result, supportive tissues of the tooth are damaged and periodontal attachment is lost. Gingival recession, formation of periodontal pockets with the presence of bleeding, and often suppuration and/or tooth mobility are evident upon clinical examination. These changes may ultimately lead to tooth loss. Mesenchymal stem cells (MSCs) are implicated in controlling periodontal disease progression and have been shown to play a key role in periodontal tissue homeostasis and regeneration. Evidence shows that MSCs interact with subgingival microorganisms and their by-products and modulate the activity of immune cells by either paracrine mechanisms or direct cell-to-cell contact. The aim of this review is to reveal the interactions that take place between microbes and in particular periodontal pathogens and MSCs in order to understand the factors and mechanisms that modulate the regenerative capacity of periodontal tissues and the ability of the host to defend against putative pathogens. The clinical implications of these interactions in terms of anti-inflammatory and paracrine responses of MSCs, anti-microbial properties and alterations in function including their regenerative potential are critically discussed based on literature findings. In addition, future directions to design periodontal research models and study ex vivo the microbial–stem cell interactions are introduced.

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Section: Microbial Effects On Other Functions Of Stem Cellscontrasting

confidence: 96%

Section: Microbial–stem Cell Interactionsmentioning

confidence: 99%

Microbial–stem cell interactions in periodontal disease

Iliopoulos

Layrolle

Apatzidou

2022

Journal of Medical Microbiology

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“…The assessments were conducted and performed in triplicates with regard to previous studies [16,17] and sample size determination formula for controlled laboratory experiments. Data were provided as mean + standard deviation.…”

Section: Discussionmentioning

confidence: 99%

“…There are other studies with non-periodontopathogen LPS from E. coli showed different results on how LPS effect stem cells in terms of reduced proliferation and growth factor secretion [16]. Therefore, as the first step of understanding HUCMSCs under periodontopathogen LPS condition, the purpose of this study was to investigate the cytotoxicity of various concentrations of Porphyromonas gingivalis LPS on the viability HUCMSCs, through MTT assay.…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

Kuntjoro

Prasetyo

Cahyani

et al. 2020

Pesqui. Bras. Odontopediatria Clín. Integr.

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To show the cytotoxicity of Porphyromonas gingivalis lipopolysaccharide (LPS) on human umbilical cord mesenchymal stem cells (HUCMSCs) to better understand the characteristics for its application in regenerative procedures under periodontopathogen LPS influence. Material and Methods: Ultrapure Porphyromonas gingivalis LPS was used in this study. This research used a frozen stock HUCMSCs, previously confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSCs were cultured and divided into two groups, the control group and LPS group with various concentrations from 25 to 0.39 µg/mL. MTT assay was done and the cells were observed and counted. The significance level was set at 5%. Results: The percentage of living HUCMSCs on LPS group were not significantly different among concentrations (p>0.05) from 25 to 0.39 µg/mL, even though there were slight mean decrease between groups, but they were not significant. The duration of 24 hours of exposure of LPS does not significantly lower HUCMSCs viability. Conclusion: LPS does not affect the viability of HUCMSCs. The lower the concentration of LPS, the higher the viability of HUCMSCs.

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“…For instance, P. gingivalis lipopolysaccharide (LPS) decreased ALP activity, OCN and Col-1 expression in PDL-MSCs [ 123 ]. LPS also reduced PDL-MSCs proliferation and expression of vascular endothelial growth factor (VEGF) [ 124 ] and stimulated expression of TLR4 and NF-ĸβ [ 125 ].…”

Section: Tissue Engineering and Periodontal Regenerationmentioning

confidence: 99%

The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium

et al. 2021

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Periodontitis and diabetes mellitus (DM) are two of the most common and challenging health problems worldwide and they affect each other mutually and adversely. Current periodontal therapies have unpredictable outcome in diabetic patients. Periodontal tissue engineering is a challenging but promising approach that aims at restoring periodontal tissues using one or all of the following: stem cells, signalling molecules and scaffolds. Mesenchymal stem cells (MSCs) and insulin-like growth factor (IGF) represent ideal examples of stem cells and signalling molecules. This review outlines the most recent updates in characterizing MSCs isolated from diabetics to fully understand why diabetics are more prone to periodontitis that theoretically reflect the impaired regenerative capabilities of their native stem cells. This characterisation is of utmost importance to enhance autologous stem cells based tissue regeneration in diabetic patients using both MSCs and members of IGF axis.

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Effect of lipopolysaccharide on cell proliferation and vascular endothelial growth factor secretion of periodontal ligament stem cells

Cited by 14 publications

References 27 publications

Microbial–stem cell interactions in periodontal disease

Microbial–stem cell interactions in periodontal disease

Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium

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