Effect of Linseed Oil Dietary Supplementation on Fatty Acid Composition and Gene Expression in Adipose Tissue of Growing Goats

Ebrahimi, Mahdi; Rajion, Mohamed Ali; Goh, Yong Meng; Sazili, Awis Qurni; Schonewille, J.T.

doi:10.1155/2013/194625

Cited by 42 publications

(43 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The injector temperature was programmed at 250 ºC, and the detector temperature was 270 ºC. The column temperature programme ran at 150 ºC for 2 min, warmed to 158 ºC at 1 ºC/min, held for 28 min, warmed to 220 ºC at 1 ºC/min, and then held for 20 min to achieve satisfactory separation (Ebrahimi et al, 2013). The peaks of samples were identified, and concentrations calculated based on the retention time and peak area of known standards (Sigma Chemical).…”

Section: Methodsmentioning

confidence: 99%

The effect of breed on fatty acid composition of subcutaneous adipose tissues in fat-tailed sheep under identical feeding conditions

Maleki¹,

Kafilzadeh²,

Meng³

et al. 2015

SA J. An. Sci.

View full text Add to dashboard Cite

A study was conducted to evaluate the fatty acid (FA) profile of subcutaneous adipose tissue and tailfat of two fat-tailed sheep breeds under identical feeding conditions. Twelve male lambs from two breeds, Sanjabi (n = 6), weighing 23.3 ± 0.48 kg, and Mehraban (n = 6), weighing 26.1 ± 2.14 kg, were used in this investigation. All animals were weighed and slaughtered at the end of a 90-day fattening period and FA composition of subcutaneous adipose tissue and tailfat was determined. Significant breed differences in total FA, total polyunsaturated fatty acid (PUFA) and n-6PUFA contents were observed in the subcutaneous adipose tissue, but not in the tailfat. Breed differences were observed in the conjugated linoleic acid (CLA) content of subcutaneous adipose tissue, with higher levels in Sanjabi than in Mehraban lambs. Linoleic and α-linolenic acid of subcutaneous adipose were significantly higher in the Sanjabi breed than in the Mehraban breed. Thus, adipose tissue from fat-tailed Sanjabi lamb has a higher proportion of health-promoting linoleic and α-linolenic acids, unsaturated fatty acids (UFA) and CLA, suggesting that the Sanjabi breed could be used for producing healthier lamb products.

show abstract

Section: Methodsmentioning

confidence: 99%

The effect of breed on fatty acid composition of subcutaneous adipose tissues in fat-tailed sheep under identical feeding conditions

Maleki¹,

Kafilzadeh²,

Meng³

et al. 2015

SA J. An. Sci.

View full text Add to dashboard Cite

show abstract

“…The group with the highest mean ΔCT value (lowest gene expression) per amplified gene target was set to zero and the mean ΔCT values of the other groups were set relative to this calibrator (ΔΔCT). The ΔΔCT values were calculated as powers of 2 (-2ΔΔCT), to account for the exponential doubling of the PCR as described by Ebrahimi et al [32].…”

Section: Liver Gene Expressionmentioning

confidence: 99%

Diet high in α-linolenic acid up-regulate PPAR-α gene expression in the liver of goats

Ebrahimi

Rajion

Goh

et al. 2015

Electronic Journal of Biotechnology

View full text Add to dashboard Cite

Background: There is little information on the effects of diets containing high α-linolenic acid (C18:3n-3) on liver lipid composition and lipogenic gene expressions. In this study fourteen goats (Capra aegagrus hircus) were fed either a flaxseed oil (FSO) supplemented diet containing high α-linolenic acid or a control diet without added flaxseed oil (CON) for 100-d to evaluate the effects on liver lipid composition and the gene expression of peroxisome proliferator-activated receptors (PPAR-α) and stearoyl-CoA-desaturase (SCD) in the liver. Results: An increase in the levels of C18:3n-3 and C20:5n-3, C22:5n-3, C22:6n-3 was observed in the liver of FSO-treated goats. There was a significant (P b 0.05) up-regulation of PPAR-α gene expression and downregulation of SCD gene in the liver of goats fed the high α-linolenic acid diet. Conclusions: In conclusion, genes associated with the control of fatty acid (FA) conversion (SCD and PPAR) were affected by the α-linolenic acid supplementation in the goat diet. It is suggested that PPAR-α is the key messenger responsible for the translation of nutritional stimuli into changes in hepatic gene expression.

show abstract

“…The methylated fatty acids were separated by Agilent 7890A gas chromatography (Agilent Technologies) as described by Ebrahimi et al (2013).…”

Section: Chemical Analysismentioning

confidence: 99%

The Effects of Adding Lactic Acid Bacteria and Cellulase in Oil Palm(Elais GuineensisJacq.) Frond Silages on Fermentation Quality, Chemical Composition and in Vitro Digestibility

Ebrahimi

Rajion

Goh

et al. 2014

Italian Journal of Animal Science

View full text Add to dashboard Cite

The main objective of the current study was to evaluate whether oil palm frond (OPF) can be successfully ensiled without or with the additives cellulase or lactic acid bacteria (LAB). Thus, fresh OPF was ensiled either without additives or with cellulase or LAB or their combination. Ensiling was carried out by storing 2 kg samples in airtight glass jars at 25-30°C for 12 weeks. Thereafter, the silage samples were subjected to proximate analyses, an in vitro digestibility assay and measures on selected indices of fermentation. Fermentation of OPF without additives appeared to be unsuccessful as both pH and ammonia content were too high (4.9 and 9.9%, respectively). In contrast, the use of cellulase or LAB resulted in silages with a pH<4.5 and ammonia fractions <8.4%, but the lowest values were found when both cellulase and LAB were used, i.e. pH=4.1 and ammonia fraction=6.7%. In vitro digestibility of dry matter was significantly higher in the cellulase treated silages. The process of ensiling was associated with both a significant decrease of the fat content of OPF and a significant change of the fatty acid profile. However, the proportions of major fatty acids (C16:0 and C18:2n-6) were not affected by the process of ensiling. In conclusion, the use of cellulase additive appears a practical tool to safeguard the process of fermentation. Using a cellulase enzyme or its combination with LAB improves the fermentation profile and increases the nutritional value of the OPF silage.

show abstract

Effect of Linseed Oil Dietary Supplementation on Fatty Acid Composition and Gene Expression in Adipose Tissue of Growing Goats

Cited by 42 publications

References 55 publications

The effect of breed on fatty acid composition of subcutaneous adipose tissues in fat-tailed sheep under identical feeding conditions

The effect of breed on fatty acid composition of subcutaneous adipose tissues in fat-tailed sheep under identical feeding conditions

Diet high in α-linolenic acid up-regulate PPAR-α gene expression in the liver of goats

The Effects of Adding Lactic Acid Bacteria and Cellulase in Oil Palm(Elais GuineensisJacq.) Frond Silages on Fermentation Quality, Chemical Composition and in Vitro Digestibility

Contact Info

Product

Resources

About