Effect of light intensity on physiological changes, carbon allocation and neutral lipid accumulation in oleaginous microalgae

Optimizing light regimes on growth and lipid accumulation in Ankistrodesmus fusiformis H1 for biodiesel production

“…5a). The progressive reduction of Fv/Fm can be explained by the aggravated oxidative stress and the nutrient consumption in microalgae (He et al, 2015b). At the same time, the content of Chl a decreased, but carotenoid contents increased in optimized culture (Fig.…”

“…The determination of photosynthetic activity and pigment contents was measured according to He et al (2015b). Protein content was analyzed following the method of Bradford (1976), 2 mL sample of cells were obtained by centrifugation and incubated in NaOH solution for one hour at 90°C.…”

“…As for photoperiods and light intensities, under longer light duration time and higher light intensity, the level of SFA and MUFA increased, PUFA decreased. These results were consistent with that previously reported for Chlorella sp., M. dybowskii and Nannochloropsis oceanic (He et al, 2015b;Xiao et al, 2015). In general, A. fusiformis H1 mainly contained the lipid profile of C16 and C18 fatty acids which were main candidates for biodiesel production (Knothe, 2011;He et al, 2015a,b;Xiao et al, 2015).…”

“…Photoperiods and light intensity are approved to be the prime and critical factors that determine the growth rate of microalgal cultures (Parmar et al, 2011;Wahidin et al, 2013;He et al, 2015b). Most process of the carbon fixation, glucan biosynthesis and lipid biosynthesis occur during the light period.…”

“…motisis, the TCA cycle and lipid b-oxidation occur during the dark period (Chauton et al, 2013). Numerous studies have reported the lipid content in microalgae (e.g., Nannochloropsis, Chlorella sp., Monoraphidium dybowskii) can accumulatively increase under higher light intensity (Wahidin et al, 2013;Gwak et al, 2014;He et al, 2015b), but it had seldom reported for Ankistrodesmus sp. The lipid in microalgae primarily constitute of polar structural lipids (such as phospholipids and glycolipids) together with storage neutral lipids especially TAG which is the most important component for biodiesel production (Olofsson et al, 2012).…”