Effect of Levofloxacin and Vitamin C on Bacterial Adherence and Preformed Biofilm on Urethral Catheter Surfaces

El-Gebaly, Eman; Essam, Tamer; Hashem, Shabaan; El-Baky, Rehab A.

doi:10.4172/1948-5948.1000083

Cited by 26 publications

(28 citation statements)

References 15 publications

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“…Several authors have already described the bacteriostatic activity of AA against Staphylococcus aureus, 18,19) as well as its synergy with some antibiotics. 20,21) Interesting results were obtained for Pseudomonas aeruginosa 22,23) alone and in the presence of an antibiotic 24) and for Candida albicans. [25][26][27] Escherichia coli seems to be inhibited in some conditions in the presence of AA.…”

Section: Discussionmentioning

confidence: 99%

Selected Physicochemical and Biological Properties of Ethyl Ascorbic Acid Compared to Ascorbic Acid

Golonka

Oleksy

Junka

et al. 2017

Biological & Pharmaceutical Bulletin

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The aim of the work was the evaluation of selected biological and physicochemical, applicative properties of ethylated ascorbic acid (AAE) compared to ascorbic acid (AA). Thermogravimetry (TG), differential thermogravimetry (DTG), and differential thermal analysis (DTA) were conducted, followed by the evaluation of AAE decomposition by the UV-Vis spectroscopic method including the influence of temperature and pH. Scavenging, antimicrobial activity and cytotoxicity against L929 fibroblasts were also performed. The difference in mass loss between AA and AAE was 30% via TG. DTA revealed characteristic exothermic and endothermic effects. The AAE solution was more thermally stable than AA. The calculated zero-order rate constants of free-radical scavenging kinetics for AAE were in the range of 4.9 10 3 -1.35 10 2 s 1 . The activation energy for the process was 11,2281 kJ/mol. AAE was active against Staphylococcus (S.) aureus and Enterococcus (E.) faecalis and acted stronger against Candida (C.) albicans than AA. The concentrations of AA ≥2.5% were cytotoxic, whereas in the case of AAE, a 10% concentration was considered cytotoxic. DTG enables the detailed differentiation between AA and AAE. AAE in aqueous solution is more stable compared to AA. The antioxidant activity of AAE is significant. However, the reaction with 2,2-diphenyl-1-picrylhydrazyl (DPPH) indicates prolonged activity compared to AA. Variability in the antimicrobial activity of AAE may find practical application in the pharmaceutical and cosmetic industries. The potential for applicative aims may be supported by the relatively low in vitro toxicity of AAE.

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Section: Discussionmentioning

confidence: 99%

Selected Physicochemical and Biological Properties of Ethyl Ascorbic Acid Compared to Ascorbic Acid

Golonka

Oleksy

Junka

et al. 2017

Biological & Pharmaceutical Bulletin

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“…Double fold serially diluted MNPs (OA-MNPs and APTMS-MNPs) were added in all wells except in negative control wells and incubated at 37 ∘ C for 24 h to evaluate the effect of MNPs on preformed biofilms. MNPs were discarded and the remaining biofilms were stained with CV as previously described [26]. diluted MNPs (except negative control wells) and incubated at 37 ∘ C for 24 h. Residual living cells were treated with 20 L sterile 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (5 mg/mL) at 37 ∘ C for 4 h. MTT was discarded and plates were washed with PBS three times.…”

Section: Effect On Preformedmentioning

confidence: 99%

Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

Shebl

Farouk

Azzazy

2017

Journal of Nanomaterials

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Unmodified magnetic nanoparticles (MNPs) lack antibacterial potential. We investigated MNPs surface modifications that can impart antibacterial activity. Six MNPs species were prepared and characterized. Their antibacterial and antibiofilm potentials, surface affinity, and cytotoxicity were evaluated. Prepared MNPs were functionalized with citric acid, amine group, amino-propyl trimethoxy silane (APTMS), arginine, or oleic acid (OA) to give hydrophilic and hydrophobic MNPs with surface charge ranging from −30 to +30 mV. Prepared MNPs were spherical in shape with an average size of 6-15 nm. Hydrophobic (OA-MNPs) and positively charged MNPs (APTMS-MNPs) had significant concentration dependent antibacterial effect. OA-MNPs showed higher inhibitory potential against S. aureus and E. coli (80%) than APTMS-MNPs (70%). Both particles exhibited surface affinity to S. aureus and E. coli. Different concentrations of OA-MNPs decreased S. aureus and E. coli biofilm formation by 50-90%, while APTMS-MNPs reduced it by 30-90%, respectively. Up to 90% of preformed biofilms of S. aureus and E. coli were destroyed by OAMNPs and APTMS-MNPs. In conclusion, surface positivity and hydrophobicity enhance antibacterial and antibiofilm properties of MNPs.

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“…Similar results against mature biofilms of uropathogenic bacteria (E. coli, Klebsiella spp., Citrobacter spp., Enterobacter spp., Proteus spp., and Pseudomonas spp.) have been reported by El-Gebaly et al [31]. On the contrary, other studies [30,[32][33][34] have found that ascorbic acid did not inhibit the growth of E. coli and P. aeruginosa but made them more resistant to antimicrobials and physical disinfection.…”

Section: Discussionmentioning

confidence: 84%

Ascorbic Acid Changes Growth of Food-Borne Pathogens in the Early Stage of Biofilm Formation

et al. 2020

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Since bacterial biofilm may contribute to the secondary contamination of food during the manufacturing/processing stage there is a need for new methods allowing its effective eradication. Application of food additives such as vitamin C already used in food industry as antioxidant food industry antioxidants may be a promising solution. The aim of this research was evaluation of the impact of vitamin C (ascorbic acid), in a range of concentrations 2.50 µg mL−1–25.0 mg mL−1, on biofilms of Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes strains isolated from food. The efficacy of ascorbic acid was assessed based on the reduction of optical density (λ = 595 nm). The greatest elimination of the biofilm was achieved at the concentration of vitamin C of 25.0 mg mL−1. The effect of the vitamin C on biofilm, however, was strain dependent. The concentration of 25.0 mg mL−1 reduced 93.4%, 74.9%, and 40.5% of E. coli, L. monocytogenes, and S. aureus number, respectively. For E. coli and S. aureus lower concentrations were ineffective. In turn, for L. monocytogenes the biofilm inhibition was observed even at the concentration of 0.25 mg mL−1. The addition of vitamin C may be helpful in the elimination of bacterial biofilms. Nonetheless, some concentrations can induce growth of the pathogens, posing risk for the consumers’ health.

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Effect of Levofloxacin and Vitamin C on Bacterial Adherence and Preformed Biofilm on Urethral Catheter Surfaces

Cited by 26 publications

References 15 publications

Selected Physicochemical and Biological Properties of Ethyl Ascorbic Acid Compared to Ascorbic Acid

Selected Physicochemical and Biological Properties of Ethyl Ascorbic Acid Compared to Ascorbic Acid

Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

Ascorbic Acid Changes Growth of Food-Borne Pathogens in the Early Stage of Biofilm Formation

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