Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

Shebl, Rania Ibrahim; Farouk, Faten; Azzazy, Hassan M.E.

doi:10.1155/2017/3528295

“…The synthesized nanoparticles were negatively charged (− 18 mV), spherical in shape and of an average size of 5-15 nm as evident from their transmission electron microscope image (Fig. 2) [44]. The synthesized particles were subsequently surfacecoated with the lipophilic OA or β-CD [49].…”

Section: Resultsmentioning

confidence: 92%

“…The dried spot was then observed under a transmission electron microscope at electron acceleration voltage of 80 kV. MNPs were surfacecoated with oleic acid (OA) as described in [44]…”

Section: Synthesis and Surface Modification Of The Nanoparticlesmentioning

confidence: 99%

Enhancing electrode sensitivity for detection of antibiotic contamination in water using functionalized magnetic nanoparticles

Farouk

¹

,

Niessen²

2020

SN Appl. Sci.

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Portable electrochemical sensors for the detection of antibiotic (AB) contamination are gaining interest as point of care devices and as in-field analytical sensors. In that respect, the low cost and high sensitivity of the sensors is a major benefit. In this study, a low-cost indicator electrode was designed against neomycin (NEO), gatifloxacin (GAT) and sulfathiazole sodium (STNa) as examples of ABs with high probability of contaminating animal feed/water. Ionophore functionalized magnetic nanoparticles were incorporated into the electrode structure to enhance its performance. The detector was able to discriminate (within 10 s) AB clean from AB contaminated water in ranges of 10 −3-10 −8 M, of 10 −2-10 −8 M, and of 10 −2-10 −13 M for NEO, GAT and STNa, respectively. The developed electrodes can be fabricated at low cost; with promising fast and high sensitivity for the detection of AB contamination in water.

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“…Since hexane is a non-polar solvent, it has no charge. Thus, the conjugated surface of AuNWs with negatively charge of the hydrophobic OA, yield a negative surface charge of the AuNWs system [82,83]. As a result, no electrostatic interactions due to absent of different charges in the solution between the cell surface and as -synthesised AuNWs suspended in hexane.…”

Section: Cytotoxicity Evaluation Of As-synthesised and Commercial Aunwsmentioning

confidence: 99%

Synthesis, Characterisation and Cytotoxicity of Gold Nanowires for Ultra-Sensitive Biosensor Development

Lah

¹

,

Gray

²

,

Trigueros

³

2020

Preprint

0

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With the long-term goal of developing an ultra-sensitive microcantilever-based nano biosensor for versatile biomarker detection, new controlled bioreceptor-analytes systems are being explored to overcome the disadvantages of conventional ones. Gold nanowires (AuNWs) have been used as a probe to overcome the tolerance problem that occurs in response to changes in environmental conditions. However, the cytotoxicity of AuNWs is still unclear. Here, we examined the cytotoxicity of AuNWs systems using both commercial and as-synthesised AuNWs. In vitro experiments show that commercial AuNWs with an average quoted length of 6 µm are highly toxic against Gram-negative Escherichia coli (E. coli) at 50 µg/ml. However, this toxicity is due to the presence of CTAB surfactant not by the nanostructure. Conversely, the as-synthesised AuNWs with an average length of 9.5 µm show non-cytotoxicity even at the maximum viable concentration (330µg/ml). These findings may lead to the development of potentially life-saving cytotoxicity -free nano biosensors for an early diagnostic of potential diseases.

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“…Since hexane is a non-polar solvent, it has no charge. Thus, the conjugated surface of AuNWs with negatively charge of the hydrophobic OA, yield a negative surface charge of the AuNWs system [86,87]. As a result, no electrostatic interactions due to absent of different charges in the solution between the cell surface and as -synthesised AuNWs suspended in hexane.…”

Section: Cytotoxicity Evaluation Of As-synthesised and Commercial Aunwsmentioning

confidence: 99%

Synthesis, Characterisation and Cytotoxicity of Gold Nanowires for Ultra-Sensitive Biosensor Development

Lah

¹

,

Gray

²

,

Trigueros

³

2020

Preprint

0

View full text Add to dashboard Cite

With the long-term goal of developing an ultra-sensitive microcantilever-based nano biosensor for versatile biomarker detection, new controlled bioreceptor-analytes systems are being explored to overcome the disadvantages of conventional ones. Gold nanowires (AuNWs) have been used as a probe to overcome the tolerance problem that occurs in response to changes in environmental conditions. However, the cytotoxicity of AuNWs is still unclear. Here, we examined the cytotoxicity of AuNWs systems using both commercial and as-synthesised AuNWs. In vitro experiments show that commercial AuNWs with an average quoted length of 6 µm are highly toxic against Gram-negative Escherichia coli (E. coli) at 50 µg/ml. However, this toxicity is due to the presence of CTAB surfactant not by the nanostructure. Conversely, the as-synthesised AuNWs with an average length of 9.5 µm show non-cytotoxicity even at the maximum viable concentration (330µg/ml). These findings may lead to the development of potentially life-saving cytotoxicity -free nano biosensors for an early diagnostic of potential diseases.

show abstract

Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

Cited by 32 publications

References 57 publications

Enhancing electrode sensitivity for detection of antibiotic contamination in water using functionalized magnetic nanoparticles

Enhancing electrode sensitivity for detection of antibiotic contamination in water using functionalized magnetic nanoparticles

Synthesis, Characterisation and Cytotoxicity of Gold Nanowires for Ultra-Sensitive Biosensor Development

Synthesis, Characterisation and Cytotoxicity of Gold Nanowires for Ultra-Sensitive Biosensor Development

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