Effect of leukemia inhibitory factor on bovine embryos produced in vitro under chemically defined conditions

Sirisathien, Saksiri; Hernández–Fonseca, Hugo; Bosch, Pablo; Hollet, B.R; Lott, J.D; Brackett, Benjamin G.

doi:10.1016/s0093-691x(02)01258-x

Cited by 29 publications

(45 citation statements)

References 35 publications

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“…The role of hLIF in bovine embryo culture has been controversial in previous studies [8][9][10][11] in which the effects of different supplements, such as serum, synthetic molecules and BSA were examined. In the present study, embryonic development was impaired by hLIF, while the number of ICM cells was reduced by mLIF, in disagreement with the effects described by Yamanaka et al for bLIF [6,7].…”

Section: Discussionmentioning

confidence: 99%

“…Treatments were as follows: (1) recombinant mLIF (L5158); (2) recombinant hLIF (L5283); (3) no LIF (negative control). The concentration of LIF used was 100 ng ml À1 as described in recent studies using hLIF [8,12].…”

Section: In Vitro Embryo Culturementioning

confidence: 99%

“…However, the use of hLIF has yielded controversial results as compared to bLIF, and mLIF has not been used for this purpose. When added from the morula stage, hLIF has been reported to have no effect on subsequent development [8,9]. During the course of blastocyst formation, hLIF has been found to stimulate expansion and hatching [8,10], but other authors report no comparable effect [11].…”

Section: Introductionmentioning

confidence: 99%

“…When added from the morula stage, hLIF has been reported to have no effect on subsequent development [8,9]. During the course of blastocyst formation, hLIF has been found to stimulate expansion and hatching [8,10], but other authors report no comparable effect [11]. It has also been described that the number of cells in the inner cell mass (ICM) and total cell numbers increase in the presence of hLIF, although this led to no improvement in pregnancy and survival rates to cryopreservation [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…During the course of blastocyst formation, hLIF has been found to stimulate expansion and hatching [8,10], but other authors report no comparable effect [11]. It has also been described that the number of cells in the inner cell mass (ICM) and total cell numbers increase in the presence of hLIF, although this led to no improvement in pregnancy and survival rates to cryopreservation [8,9]. Recently, hLIF has been reported to have adverse effects on embryonic development kinetics, morphology, cell counts and expression of Oct4 and laminin, having no apparent influence on the subsequent formation of outgrowths [12].…”

Section: Introductionmentioning

confidence: 99%

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Effects of human versus mouse leukemia inhibitory factor on the in vitro development of bovine embryos

et al. 2007

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Leukemia inhibitory factor (LIF) is a cytokine that shows conflicting effects on in vitro produced (IVP) bovine embryos. Bovine LIF (bLIF) has been cloned and used in culture, but there is no commercially available bLIF. Thus, researchers use human LIF (hLIF) to supplement the culture medium for bovine embryos because of its greater sequence homology compared to murine LIF (mLIF). We compared the effects of mLIF and hLIF on the development of bovine embryos in culture with the effects described for bLIF. Oocytes were matured and fertilized in vitro and cultured in modified synthetic oviduct fluid with BSA. On Day 6 postinsemination, morulae were cultured for 48 h in the presence of: (1) mLIF, 100 ng ml À1 ; (2) hLIF, 100 ng ml À1 ; or (3) no LIF. Reduced blastocyst rates were observed on Day 8 for hLIF at the middle and expanded stages, while mLIF had no effect. In contrast, Day 8 blastocysts showed decreased cell counts both in terms of inner cell mass (ICM) and ICM/total cell proportions in the presence of mLIF, while hLIF had no effect. No changes were seen in trophectoderm (TE) and total cell counts. The increased hatching rates and TE cell counts previously described for bLIF, together with the disparate effects exhibited by hLIF and mLIF during blastocyst formation indicate these compounds are inappropriate to replace bLIF. We recommend that heterospecific LIF should not be used to supplement the culture medium for bovine embryo or embryonic stem cells.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Embryo Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of human versus mouse leukemia inhibitory factor on the in vitro development of bovine embryos

et al. 2007

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Effect of leukemia inhibitory factor (LIF) on in vitro produced bovine embryos and their outgrowth colonies

Vejlsted¹,

Avery²,

Gjørret³

et al. 2005

Mol. Reprod. Dev.

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In vitro produced (IVP) bovine embryos were subjected to in vitro culture with or without 1000 U/ml human recombinant leukemia inhibitory factor (LIF) added to the culture medium from Days 5 to 8 post insemination (p.i.). Resulting blastocysts were subsequently plated intact on mouse feeder cells in a medium with or without LIF. Significantly more embryos reached the hatched blastocyst stage, and the number of blastocysts with excellent morphology was significantly higher, when LIF was omitted. At Day 8 p.i., total cell count (TCC) and inner cell mass (ICM) cell count was significantly higher in embryos cultured without LIF. In embryos cultured with LIF, cytoplasmic vesicles and lipid droplets were abundant and a decreased expression of both Oct4 and laminin could be observed. Initial hypoblast formation was revealed in almost 1/3 of the LIF-cultured blastocysts whereas this feature was evident in 2/3 of the blastocysts cultured in the absence of LIF. Overall, almost 60% of the blastocysts cultured without LIF formed outgrowth colonies (OCs) when plated on feeders, whereas this phenomenon was only observed in 30% of the blastocysts cultured in the presence of LIF. A tendency for retaining a tightly packed central growth of putative ICM-derived cells was observed, when attachment to the feeder layer was initiated close to the embryonic pole of the blastocyst. At Day 8 of outgrowth culture, approximately 20% of the colonies contained a central core of putative ICM-derived cells appearing large enough for mechanical isolation and further subculture. Immunohistochemical labeling for Oct4 revealed staining of both trophectodermal and ICM-derived cells. The presence of LIF in the outgrowth culture medium did not have any apparent effect on the plating efficiency or colony type. In conclusion, LIF had an adverse effect on in vitro embryonic development when added to the culture medium in the period from Days 5 to 8 p.i., whereas it had no apparent effect on the OCs subsequently formed from such embryos.

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Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3

Dang‐Nguyen

Haraguchi

Kikuchi

et al. 2013

Molecular Reproduction Devel

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We produced recombinant porcine leukemia inhibitory factor (pLIF) and examined its effect on in vitro maturation (IVM) of porcine oocytes and their developmental competence after in vitro fertilization. Porcine cumulus-oocyte complexes (COCs) were matured in a medium supplemented with pLIF during the first 22 hr, last 22 hr, or entire 44 hr duration of IVM. Oocytes in all groups tended to show enhanced nuclear maturation rates by the metaphase II (MII) stage (76.1%, 82.1%, and 86.6%, respectively) compared to the without-pLIF treatment group (69.6%, control). A significant increase in MII rate (P < 0.05) and obvious induction of cumulus expansion were observed over the whole time span (44 hr) in the IVM group. When cumulus cells were removed at 22 hr and denuded oocytes were further cultured, pLIF showed no effect on maturation rate. Oocytes matured in pLIF-supplemented medium showed a tendency for more rapid blastocyst development (21.1% vs. 16.2%, P = 0.0715). Examination of transcripts and proteins of the LIF signaling pathway in COCs revealed that LIF, LIF receptors, and signal transducer and activator of transcription 3 (STAT3) are present in both cumulus cells and oocytes. The amount of phosphorylated STAT3 (p-STAT3) markedly increased in both cumulus cells and oocytes cultured in pLIF-supplemented media, although oocyte p-STAT3 disappeared after 44 hr of IVM. These results suggest that the LIF/STAT3 pathway is functional during IVM of porcine oocytes, and supplementing pLIF in the IVM medium can improve oocyte maturation by activating this pathway.

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Effect of leukemia inhibitory factor on bovine embryos produced in vitro under chemically defined conditions

Cited by 29 publications

References 35 publications

Effects of human versus mouse leukemia inhibitory factor on the in vitro development of bovine embryos

Effects of human versus mouse leukemia inhibitory factor on the in vitro development of bovine embryos

Effect of leukemia inhibitory factor (LIF) on in vitro produced bovine embryos and their outgrowth colonies

Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3

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