Effect of lectins on chondrogenesis of cultured quail limb bud cells

Matsutani, Etsuya; Kuroda, Yukiaki

doi:10.1016/0012-1606(82)90341-4

Cited by 9 publications

(4 citation statements)

References 14 publications

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“…Lastly, we investigated the effects of increased cell proliferative activity and cell density on morphogenesis at the sites flanked by Shh + - Fgf8 + domains. We hypothesized that the increase in cell density leads to cartilage differentiation since it was shown that higher cell density leads to cartilage differentiation in chicken limb bud culture 21 . First, we investigated gene expression patterns in the regenerating blastema on both longitudinal and transverse sections, in which the first digit started differentiating (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Variable Shh and Fgf8 positioning in regenerating axolotl limb guarantees consistent limb morphogenesis in different limb sizes

Furukawa

Yamamoto

Kashimoto

et al. 2022

Preprint

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Limb regeneration in Ambystoma mexicanum occurs in various sizes of fields and can recreate consistent limb morphology. It was not known what mechanism supports such stable limb morphogenesis regardless of size. Limb regeneration in urodele amphibians has been basically considered to recapitulate the limb developmental processes. Many molecules in the limb developmental processes are conserved with other tetrapods. SHH and FGF8 play important roles in the morphogenesis of limbs among them. Focusing on these two factors, we investigated the detailed expression pattern of Shh and Fgf8 in the various sizes of blastema in axolotl limb regeneration. Fgf8 is expressed in the anterior side of a blastema and Shh is expressed in the posterior side. These are maintained in a mutually dependent manner. We also clarified that the size of Shh and Fgf8 expression domains were scaled as the size of the blastemas increased. However, it was found that the secretion and working range of SHH were kept constant. We also found that the consistent SHH secretion range contributed to promoting cell proliferation and the first digital cartilage differentiation near the Shh expression domain. This would be a reasonable system to guarantees constant limb morphogenesis regardless of the blastema size. We also showed that the Shh-Fgf8 expression domain was shifted posteriorly as the digital differentiation progressed. Consistently, slowing the timing of blocking Shh signaling resulted in morphological defects that could be observed in only posterior digits. The revealed posteriorly shifting Shh-Fgf8 domain might explain urodele specific digit formation, in which digits are added posteriorly.

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Section: Resultsmentioning

confidence: 99%

“…The mitogenic activity of SHH and FGF8 likely contributes to the increasing cell density. We hypothesized that the increase in cell density leads to cartilage differentiation since it was shown that higher cell density leads to cartilage differentiation in chicken limb bud culture 27 .…”

Section: Digit Formation By the Relationship Between Shh And Fgf8mentioning

confidence: 99%

Variable Shh and Fgf8 positioning in regenerating axolotl limb guarantees consistent limb morphogenesis in different limb sizes

Furukawa

Yamamoto

Kashimoto

et al. 2022

Preprint

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“…Articular cartilage progenitors from immature cartilage were cultured as high-density cell pellets for 21 days in the presence of minimal chondrogenic culture medium that was supplemented with previously characterized chondrogenic factors, TGFb1-3 (10 ng/mL), BMP 2/9 (100 ng/mL), dexamethasone (Dex), CNP, db-cAMP, concavalin-A (ConA), ethanol (EtOH), and CCl at concentrations previously shown to induce differentiation in receptive mesenchymal cells [34][35][36][37][38][39][40][41][42][43]. Histologic examination of toluidine blue-stained sections of cartilage pellets showed an increasing gradation in size and staining intensity from ethanol to BMP9-treated chondroprogenitors (Fig.…”

Section: Resultsmentioning

confidence: 99%

Bone Morphogenetic Protein-9 Is a Potent Chondrogenic and Morphogenic Factor for Articular Cartilage Chondroprogenitors

Morgan

Bauza‐Mayol

Gardner

et al. 2020

Stem Cells and Development

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Articular cartilage contains a subpopulation of tissue-specific progenitors that are an ideal cell type for cell therapies and generating neocartilage for tissue engineering applications. However, it is unclear whether the standard chondrogenic medium using transforming growth factor beta (TGFb) isoforms is optimal to differentiate these cells. We therefore used pellet culture to screen progenitors from immature bovine articular cartilage with a number of chondrogenic factors and discovered that bone morphogenetic protein-9 (BMP9) precociously induces their differentiation. This difference was apparent with toluidine blue staining and confirmed by biochemical and transcriptional analyses with BMP9-treated progenitors exhibiting 11-fold and 5-fold greater aggrecan and collagen type II (COL2A1) gene expression than TGFb1-treated progenitors. Quantitative gene expression analysis over 14 days highlighted the rapid and phased nature of BMP9-induced chondrogenesis with sequential activation of aggrecan then collagen type II, and negligible collagen type X gene expression. The extracellular matrix of TGFb1-treated progenitors analyzed using atomic force microscopy was fibrillar and stiff whist BMP9-induced matrix of cells more compliant and correspondingly less fibrillar. Polarized light microscopy revealed an annular pattern of collagen fibril deposition typified by TGFb1treated pellets, whereas BMP9-treated pellets displayed a birefringence pattern that was more anisotropic. Remarkably, differentiated immature chondrocytes incubated as high-density cultures in vitro with BMP9 generated a pronounced anisotropic organization of collagen fibrils indistinguishable from mature adult articular cartilage, with cells in deeper zones arranged in columnar manner. This contrasted with cells grown with TGFb1, where a concentric pattern of collagen fibrils was visualized within tissue pellets. In summary, BMP9 is a potent chondrogenic factor for articular cartilage progenitors and is also capable of inducing morphogenesis of adult-like cartilage, a highly desirable attribute for in vitro tissue-engineered cartilage.

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“…Succinyl Con A promoted chondrogenesis in cultured quail limb bud cells by enhancing aggregate formation (22). A chick endogenous lectin, which was specific for beta-D-galactoside, also promoted chondrogenesis in limb bud cells (23).…”

Section: C Some Other Factors Affecting Chondrogenesismentioning

confidence: 99%

Growth and Differentiation of Limb Bud Cells in Vitro: Implications for Limb Pattern Formation

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1990

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Effect of lectins on chondrogenesis of cultured quail limb bud cells

Cited by 9 publications

References 14 publications

Variable Shh and Fgf8 positioning in regenerating axolotl limb guarantees consistent limb morphogenesis in different limb sizes

Variable Shh and Fgf8 positioning in regenerating axolotl limb guarantees consistent limb morphogenesis in different limb sizes

Bone Morphogenetic Protein-9 Is a Potent Chondrogenic and Morphogenic Factor for Articular Cartilage Chondroprogenitors

Growth and Differentiation of Limb Bud Cells in Vitro: Implications for Limb Pattern Formation

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