Growth and Differentiation of Limb Bud Cells <i>in Vitro</i>: Implications for Limb Pattern Formation

Ide, Hiroyuki

doi:10.1111/j.1440-169x.1990.00001.x

Cited by 12 publications

(4 citation statements)

References 40 publications

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“…Indeed, like tooth development limb development involves and depends on reciprocal epithelial-mesenchymal interactions, polarized growth, mesenchymal condensations, proliferation of specific cell populations, and establishment of multi-axis symmetries. In the developing limb, two specialized areas, the apical ectodermal ridge (AER) and the zone of polarizing activity (ZPA), exert key roles in development (reviewed by Ide, 1990;Tabin, 1991). In particular, the ZPA, a small group of cells located at the posterior margin of the limb, acts as a source of signalling factors, such as Sonic hedgehog (Shh), and determines the antero-posterior axis of the limb (Riddle et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Polarizing activity, Sonic hedgehog , and tooth development in embryonic and postnatal mouse

Koyama

Yamaai

Iseki

et al. 1996

Developmental Dynamics

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Section: Introductionmentioning

confidence: 99%

Polarizing activity, Sonic hedgehog , and tooth development in embryonic and postnatal mouse

Koyama

Yamaai

Iseki

et al. 1996

Developmental Dynamics

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“…Retinoic acid IRA), a metabolite of vitamin A, is known to profoundly affect cell proliferation, cell differentiation, and morphogenesis in several vertebrate embryonic tissues including the neural crest (Durston et al, 1989;Ide, 1990;Tickle, 1991;Morriss-Kay, 1992, 1993Drysdale and Crawford, 1994). Both RA receptors and cellular RA binding proteins are expressed in neural crest derivatives (Dencker et al, 1990;Maden et al, 1991;Rowe et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Role of retinoic acid in mouse neural crest cell development in vitro

Morita

1995

Developmental Dynamics

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This study examines the role of retinoic acid (RA) in mouse neural crest cell development in culture. We have compared the effects of RA on the developmental behavior of mouse neural crest cells from different axial levels, that is the mesencephalic (cranial) and sympathoadrenal (trunk) levels by colony assay using antibodies against cell-type-specific markers. In control cultures in the absence of RA, the efficiency of colony formation by cranial neural crest cells was considerably lower than in colony cultures of trunk neural crest cells. Pulse-labelling experiments using 5-bromo-2'-deoxyuridine (BrdU) showed that the proportion of neural crest cells that incorporated BrdU was significantly smaller in day 5 cultures of cranial neural crest cells in the absence of RA compared to cultures from the trunk level. However, BrdU-incorporation matched the labelling observed in trunk crest cell cultures when RA was added to the culture medium. The efficiency of colony formation and the proportion of BrdU-incorporated cells in trunk crest cell cultures was similar in the presence and absence of RA. The results suggest that in the early stages of in vitro development, RA has a larger impact on proliferation andlor survival of cranial neural crest cells than of trunk neural crest cells. Moreover, the data indicate that RA significantly affects melanogenesis and the differentiation of serotonergic neurons in mouse neural crest cell cultures from both axial levels. Whereas melanogenesis was suppressed by RA treatment, serotonergic neurogenesis was promoted. Doublelabelling experiments with antibodies against BrdU and serotonin (5-HT) indicated that RA selectively promoted proliferation of these neurons at a later stage of in vitro development. Furthermore, RA acted upon both committed cells and multipotent cells. Based on the results, we conclude that RA plays multiple critical roles in mouse neural crest cell development.Q 1995 Wiley-Liss, Inc.

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“…That the poor performance of the posterior mesoderm is due to the presence of ZPA cells in postaxial mesoderm recombinants was demonstrated by the reversal of the outcomes when ZPA cells were removed from the posterior dissociated recombinants and dissociated ZPA cells mixed with preaxial dissociated recombinants. At present, it is not clear why ZPA cells show this inhibitory characteristic in recombinant limbs (see also Ide 1990). For these reasons, recombinant limbs made with wing mesoderm are best performed with anterior wing mesoderm excluding the ZPA.…”

Section: Preaxial Versus Postaxial Recombinant Limbsmentioning

confidence: 99%

The recombinant limb as a model for the study of limb patterning, and its application to muscle development

Fernandez-Teran

Piedra

Ros

et al. 1999

Cell and Tissue Research

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The recombinant limb is a model system that has proved fruitful for analyzing epithelial-mesenchymal interactions and understanding the functional properties of the components of the limb bud. Here we present an overview of some of the insights obtained through the use of this technique. Among these are the understanding that fore or hind limb identity is inherent to the limb bud mesoderm, that the apical ectodermal ridge (AER) is a permissive signaling center and that the limb bud ectoderm plays a central role in the control of dorsoventral polarity. Recombinant limb studies have also allowed the identification of the affected tissue component in several limb mutants. More recently this model has been applied to the study of regulation of gene expressions related to patterning. In this report we use recombinant limbs to analyze pattering of the Pax3 expressing limb muscle cell lineage in the early stages of limb development. In recombinant limbs made without the zone of polarizing activity (ZPA), myoblasts appear intermingled with other mesodermal cells at the beginning of the recombinant limb development. Rapidly thereafter, the muscle precursors segregate and organize around the central forming chondrogenic core of the recombinant. Although this segregation is reminiscent of that occurring during normal development, the myoblasts in the recombinant fail to proliferate appropriately and also fail to migrate distally. Consequently, the muscle pattern in the recombinant limb is defective indicating that normal patterning cues are absent. However, recombinant limbs polarized with a ZPA exhibited a larger mass of muscle cells and a more normal morphogenesis, supporting a role for this signaling center in limb muscle development. Finally, we have ruled out host somite contributions to recombinant limbs by grafting chick recombinant limbs to quail hosts. This initial report demonstrates the value of the recombinant limb model system for dissecting the environmental cues required for normal muscle limb patterning.

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Growth and Differentiation of Limb Bud Cells in Vitro: Implications for Limb Pattern Formation

Cited by 12 publications

References 40 publications

Polarizing activity, Sonic hedgehog , and tooth development in embryonic and postnatal mouse

Polarizing activity, Sonic hedgehog , and tooth development in embryonic and postnatal mouse

Role of retinoic acid in mouse neural crest cell development in vitro

The recombinant limb as a model for the study of limb patterning, and its application to muscle development

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