Effect of laser radiation on the immune response of mice against infection with secondary hydatid disease.I. Growth and development of hydatid cysts.

Ali, Asmaa A.; Mohammed, Zeena Dhabyan

doi:10.33899/edusj.2018.159319

Cited by 3 publications

(4 citation statements)

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“…Many techniques were used against infestation with hydatid disease in experiential animals, such as irradiation with laser, direct electrical current, probiotics and ultrasound (31)(32)(33)(34)(35)(36)(37), all techniques revealed considerable influence to the disease. All the techniques exhibited valuable efficiency against protoscoleces in vitro and cystic echinococcosis in mice.…”

Section: Discussionmentioning

confidence: 99%

Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice

Mohammed¹,

Ali²

2022

IJVS

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This study determined the influence of selenium nanoparticles on the vitality of the protoscoleces of Echinococcus granulosus in vitro; seven concentrations were used: 50, 100, 150, 200, 250, 350, and 500 µg/ml for different exposure times: ten, twenty, thirty, and sixty minutes, respectively. Albino mice Mus musculus were injected with protoscoleces exposed to nanoparticles at 100, 150, and 200 µg/ml concentrations for 60 minutes. In contrast, control groups were injected with non-exposed protoscoleces. Mice were dissected three, four-, and five-months post infestation. Many criteria were relied on: numbers, weights, diameters of growing hydatid cysts, and their reduction proportion. The outcomes uncovered an apparent influence of selenium nanoparticles on the viability of protoscoleces of Echinococcus granlosus by the increase in exposure time in vitro, as well as diminish in the numbers of the larvae in processed mice versus the unprocessed collection; no cysts evolved inside processed mice at the concentration 200 µg/ml, threeand four-months post-infection. In contrast, the reduction rate was 90% in mice injected with exposed protoscoleces at the concentration of 150 µg/ml next 4 and 5 months of infection.

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Section: Discussionmentioning

confidence: 99%

Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice

Mohammed¹,

Ali²

2022

IJVS

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“…Yassen (22), Ali and Salim (23) were used protoscoleces of Echinococcus granulosus exposed to electric current, and they found significant differences between treated and nontreated mice. Furthermore, Ali and Mohammed (24) tested the effect of Laser radiation on the cellular immunity in mice infected with hydatidosis, and found that the laser radiation boosted delayed-type hypersensitivity in treated animals.…”

Section: Discussionmentioning

confidence: 99%

Effect of ultrasound on protoscoleces of Echinococcus granulosus in vitro and in vivo

Ali¹,

Ramadhan²

2021

IJVS

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The current study was designed to investigate the effect of ultrasound on the protoscoleces of Echinococcus granulosus by enforcing steady numeral of frequencies during a certain interval (20000 pulse/s) (1.8 w/cm 2 ), using exposure time 30,20,15,12,10 and 5s, individually and respectively. Consequently, six albino mice groups were immunized against cystic echinococcosis, which injected with exposed protoscoleces, to acquire specific cell-mediated immunity, called delayed type-hypersensitivity (DTH) which assessment by measurement the foot pad density. The results displayed significant excess (P≤ 0.001) of DTH by increase of foot pad thickness in injected groups. The results showed maximum thickness of 1.54 mm, 1.4mm, 0.9 mm. after 3h, 24h, and 48h postinjection, respectively, that compared with the thickness of control group 1.072, 0.638, 0.328 mm, respectively, during five months of experiment. The present research exhibited the action of ultrasonication technique on the viability of Echinococcus granulosus protoscoleces in vitro. Conclusion, Ultrasound frequencies used in the present study could be have consequential impact on the cellular immunity in albino mice.

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“…Six groups of mice were used, five for each group, animals of the 1st, 2nd, 3rd and 4th groups were injected intraperitoneally with 2000 protoscoleces exposed to laser radiation, with viability 70%, 57%, 50%, 40%, the fifth positive control group was injected intraperitoneally with unexposed protoscoleces with 100% viability (15). The sixth negative control group was neither injected with protoscoleces nor exposed to laser radiation.…”

Section: Treatment Schedulementioning

confidence: 99%

“…Researchers investigated better therapeutic non-invasive alternatives, as safe killers for the protoscoleces and stimulative of the immune system, including electromagnetic radiation techniques, ultrasound, ultraviolet, Gamma ray, laser (11)(12)(13)(14)(15)(16)(17)(18), electrical current and nanoparticles (19,20).…”

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confidence: 99%

Immunity response of mice infected with laser irradiated protoscoleces of Echinococcus granulosus

Zaki¹,

Ali²

2021

J.Edu.Sci.

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The present study investigated the effect of the Neodymium-Doped Yttrium Aluminum Garnet (Nd: Y3AL5G12) Laser eradiation 1000 mw, against infection with secondary hydatid disease in BALB/c mice by inoculating the animals with protoscoleces of Echinococcus granulosus exposed to laser irradiation, for various periods 40,60,120 and 240 minutes, with viability of 70%,57%,50% and 40%, respectively, contrasted to the control set (mice inoculated with unexposed protoscoleces) along three months, depending on the evaluation of the total count of WBCs and assessment of acquired cell-mediated immunity, demonstrated by delayed type hypersensitivity test (DTH). The results showed considerable increase (p<0.01) in the total WBCs counting, in mice injected with irradiated protoscoleces, up to 13962 cell/cm 3 , in comparison to the control group 5420 cell/cm 3 , after 120 minutes, two months post infection. Moreover, there was considerable excess (p<0.01) in the foot pad thickness in treated mice, 2.94 mm after 120 minute (24hour post antigen injection), two months post infection, 2.86mm after 120 minutes (24 hours post antigen injection), 1 month post infection and 2.64 mm, 120 minutes (24 hours post antigen injection), three months post infection, compared with the control group 1.18mm, 1.80mm and 1.72mm, respectively.Results revealed that irradiation of Echinococcus granulosus protoscoleces with laser had a significant influence on the production of WBCs and stimulation of delayed-type hypersensitivity reaction in mice.

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Effect of laser radiation on the immune response of mice against infection with secondary hydatid disease.I. Growth and development of hydatid cysts.

Cited by 3 publications

References 0 publications

Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice

Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice

Effect of ultrasound on protoscoleces of Echinococcus granulosus in vitro and in vivo

Immunity response of mice infected with laser irradiated protoscoleces of Echinococcus granulosus

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