Effect of Lanostane Triterpenes from the Fruiting Bodies ofGanoderma lucidumon Adipocyte Differentiation in 3T3-L1 Cells

Abstract

Two new lanostane triterpenes, methyl lucidenate N ( 1) and T-butyl lucidenate B ( 2), were isolated from the fruiting bodies of GANODERMA LUCIDUM together with five known compounds ( 3- 7). The structures of the two new triterpenes were established as methyl 3 β,7 β-dihydroxy-4,4,14 α-trimethyl-11,15-dioxo-5 α-chol-8-en-24-oate ( 1) and T-butyl 7 β,12 β-dihydroxy-4,4,14 α-trimethyl-3,11,15-trioxo-5 α-chol-8-en-24-oate ( 2) by extensive spectroscopic studies and chemical evidence. The effect of the isolated compounds ( 1- 7) on triglyceride (TG) accumulation, an indicator of adipocyte differentiation, during the differentiation of 3T3-L1 preadipocytes was examined. T-Butyl lucidenate B ( 2) reduced the TG accumulation significantly by 72 % at 80 µM compared to the untreated group. Furthermore, compound 2 effectively suppressed the GPDH activity in the cells. Consistent with the decrease in TG accumulation and GPDH activity, compound 2 suppressed the gene expressions of PPAR γ, C/EBP α, and SREBP-1c in a dose-dependent manner during differentiation. Our findings demonstrate that the lanostane triterpenes isolated in this study contribute to the inhibitory effect of the fruiting bodies of G. LUCIDUM on adipocyte differentiation in 3T3-L1 cells.

A Comprehensive Review of the Structure Elucidation and Biological Activity of Triterpenoids from Ganoderma spp.

“…It suppressed the gene expressed of PPARγ, C/EBPα, and SREBP-1c in a dose-dependent manner during differentiation. These findings demonstrate that compound 249 contributes to the inhibitory effect on adipocyte differentiation in 3T3-L1 cells [96].…”

Isolation and Bioactivity Evaluation of Terpenoids from the Medicinal FungusGanoderma sinense

“…Because of their potential medicinal value, much attention has been paid to the search for significant pharmacological constituents from this species. For the past 30 years, over one hundred triterpenoids have been isolated and characterized from G. lucidum [2,3], some of which reveal anti-HIV-1 protease activity [4], antiviral effects [5], potential antitumor activity [6], inhibition of aldose reductase [7], and anti-inflammatory effect [8]. G. sinense, a well-known species, is widely distributed in Yunnan Province, in the southwest of China.…”

Ganoderma lucidum, the most eclectic mushroom: useful in oncology or against virus, inflammation or metabolic diseases.

“…Le GL protège le pancréas de l'aggression des îlots de Lagerhans par les radicaux libres [109]. -agent antiadipocytaire [50] ; -inhibiteur des ostéoclastes donc avec un effet probable contre l'ostéoporose [60].…”