Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

Zhang, Xue; Harjumäki, Riina; Kartal-Hodzic, Alma; Valle‐Delgado, Juan José; Österberg, Monika

doi:10.1016/j.jcis.2020.09.080

Cited by 16 publications

(13 citation statements)

References 57 publications

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“…We observed the plasmonic band displacement (∆λ) overtime from 0 to 5 h after cell seeding with and without FBS ( Figure 3C ). Basal cell culture media (DMEM without FBS) was used as a control sample and showed a limited plasmon band displacement over time with a ∆λ max ≈ 0.2 nm which can be related to unspecific absorption of components of the cell culture media ( Zhang et al, 2021 ). The cells seeded with culture media without FBS (0% FBS) showed a slightly higher plasmonic band displacement in comparison to the control sample, with a ∆λ max ≈ 0.3 nm which can correlate to a limited spreading of the cells in the surface of the sensor.…”

Section: Resultsmentioning

confidence: 99%

Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

Vila

Castro-Aguirre

López‐Muñoz

et al. 2021

Front. Bioeng. Biotechnol.

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Over the last years, optical biosensors based on plasmonic nanomaterials have gained great scientific interest due to their unquestionable advantages compared to other biosensing technologies. They can achieve sensitive, direct, and label-free analysis with exceptional potential for multiplexing and miniaturization. Recently, it has been demonstrated the potential of using optical discs as high throughput nanotemplates for the development of plasmonic biosensors in a cost-effective way. This work is a pilot study focused on the development of an integrated plasmonic biosensor for the monitoring of cell adhesion and growth of human retinal pigmented cell line (ARPE-19) under different media conditions (0 and 2% of FBS). We observed an increase of the plasmonic band displacement under 2% FBS compared to 0% conditions over time (1, 3, and 5 h). These preliminary results show that the proposed plasmonic biosensing approach is a direct, non-destructive, and real-time tool that could be employed in the study of living cells behavior and culture conditions. Furthermore, this setup could assess the viability of the cells and their growth over time with low variability between the technical replicates improving the experimental replicability.

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Section: Resultsmentioning

confidence: 99%

Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

Vila

Castro-Aguirre

López‐Muñoz

et al. 2021

Front. Bioeng. Biotechnol.

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“…Membranes having 3.1% residual proteins at their surface were found to promote higher cell density and spreading as well as a more orientated shape cell morphology compared to membranes constituted of bleached CNFs. In another work by Zhang et al [48], the real-time cell adsorption of cells onto non-modified and modified CNF films was monitored by multi-parametric surface plasmon resonance. The presence of either human recombinant laminin-L521 (natural protein of the extracellular matrix) or poly-Llysine at the surface of the CNF films resulted in enhanced attachment of human hepatocellular carcinoma cells compared to non-modified CNF films.…”

Section: Introduction To Nanocellulose In Healthcarementioning

confidence: 99%

Current international research into cellulose as a functional nanomaterial for advanced applications

et al. 2022

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This review paper provides a recent overview of current international research that is being conducted into the functional properties of cellulose as a nanomaterial. A particular emphasis is placed on fundamental and applied research that is being undertaken to generate applications, which are now becoming a real prospect given the developments in the field over the last 20 years. A short introduction covers the context of the work, and definitions of the different forms of cellulose nanomaterials (CNMs) that are most widely studied. We also address the terminology used for CNMs, suggesting a standard way to classify these materials. The reviews are separated out into theme areas, namely healthcare, water purification, biocomposites, and energy. Each section contains a short review of the field within the theme and summarizes recent work being undertaken by the groups represented. Topics that are covered include cellulose nanocrystals for directed growth of tissues, bacterial cellulose in healthcare, nanocellulose for drug delivery, nanocellulose for water purification, nanocellulose for thermoplastic composites, nanocellulose for structurally colored materials, transparent wood biocomposites, supercapacitors and batteries.

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“…The PLL is a polypeptide ionomer promoting cell adhesion in vitro cell culture. [ 13 ] The PLLGA, is an ionomer of random copolymer comprised of 80% of lysine and 20% of glutamic acid in molar ratio. The glutamic acid is a neuron stimulant which is designed in the PLLGA for neuron tissue engineering application.…”

Section: Introductionmentioning

confidence: 99%

Three‐Level Hierarchical 3D Network Formation and Structure Elucidation of Wet Hydrogel of Tunable‐High‐Strength Nanocomposites

Tseng

Wang

et al. 2022

Macro Materials & Eng

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The structural characterization of wet gel state hydrogels at multi‐length scales ranging from nanometers to micrometers is very challenging and rarely reported. Herein, tunable high‐strength nanocomposite hydrogels based on cellulose nanofibers with various cross linkers are synthesized and characterized. Synchrotron tomography and small‐angle X‐ray scattering methods are combined to quantitatively elucidate 3D hierarchically wet gel state network structures, providing an understanding on the structure–properties relationship of hydrogels. The hierarchical 3D network of wet gel demonstrates three levels of structures: 1) the bundle aggregated by cellulose nanofibers; 2) physically crosslinked bundle networks; and 3) fractal‐structure agglomerates composed of aggregated network domains. The finding of fractal agglomerate structures tuned by crosslinker characteristics provides fundamental knowledge to explain a variation of several orders of magnitude of mechanical properties and reveals different formation mechanisms, opening a new and flexible prospect for wet gel state material design and processing control.

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Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

Cited by 16 publications

References 57 publications

Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

Current international research into cellulose as a functional nanomaterial for advanced applications

Three‐Level Hierarchical 3D Network Formation and Structure Elucidation of Wet Hydrogel of Tunable‐High‐Strength Nanocomposites

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