Effect of Lagenaria siceraria fruit powder on sodium oxalate induced urolithiasis in Wistar rats

Abstract

Background:In spite of advances in the present practice of medicine, the formation and growth of calculi continues to trouble mankind, as there is no satisfactory drug to treat kidney stones. In India, many indigenous drugs are in use for the treatment of urinary calculus disease.Objective:The present study was intended to determine anti-urolithiatic effect of Lagenaria siceraria fruit powder (LSFP) against sodium oxalate (NaOx) induced urolithiasis in rats.Materials and Methods:Animals were grouped as Vehicle Group (received vehicle gum acacia 2% w/v 1 mL/kg/p.o.), NaOx Group(Sodium oxalate 70 mg/kg,i.p.), LSFP Group (500 mg/kg, p.o. LSFP suspended in gum acacia 2% + Sodium oxalate 70 mg/kg), Cystone Group (500 mg/kg, p.o. Cystone suspended in gum acacia 2% + Sodium oxalate 70 mg/kg).Result:The increased severity of microscopic calcium oxalate (CaOx) crystals deposition along with increased concentration in the kidney was seen after 7 days of NaOx (70 mg/kg, i.p.) pre-treatment. LSFP (500 mg/kg, p.o.) and standard marketed formulation Cystone (500 mg/kg, p.o.) caused a significant reversal of NaOx-induced changes in ion excretion and urinary CaOx concentration in 7 days treatment.Conclusion:From the results, it was concluded that LSFP showed beneficial effect against urolithiasis by decreasing CaOx excretion and preventing crystal deposition in the kidney tubules.

Preclinical Evaluation of Antiurolithiatic Activity ofViburnum opulusL. on Sodium Oxalate-Induced Urolithiasis Rat Model

“…In the previous studies, in which the sodium oxalate-induced urolithiasis model was used, excessive urinary excretions of uric acid and creatinine have been detected [18]. Similarly herein, uric acid and creatinine excretion were found to be high in NaOx group, when compared to the LJVO, LCJVO, and the reference groups.…”

“…Low level of urinary magnesium is also an indication of the kidney stone [18]. Reduction in urinary magnesium level was detected in the NaOx group, while LJVO, LCJVO, and Cystone treatment improved the excretion of magnesium in the present study.…”

“…Furthermore, accumulation of the CaOx crystals in the kidney increased the urinary pH, which is one of the indications of urolithiasis [18]. In the present study, the administration of NaOx increased the urine pH whereas the treatment of Cystone decreased.…”

Protective effect of ethyl acetate fraction of Biophytum sensitivum extract against sodium oxalate-induced urolithiasis in rats

“…DCM-MBS, EA-MBS, E-MBS and AQ-MBS) were evaluated for antiurolithiatic activity at the dose levels of 20 and 40 mg/kg (p.o. ), 16 for seven days treatment, 18 , 19 against sodium oxalate-induced calcium oxalate urolithiasis in rats.…”

“…Sodium oxalate-induced calcium oxalate urolithiasis model was used to evaluate antiurolithiatic effect of fractions of MBS in rats. 18 , 19 Animals were randomly divided into ten groups each containing six animals. Group I served as vehicle control, animals of this group were maintained on standard rat food and drinking water ad libitum and were received vehicle, i.e.…”