Protective effect of ethyl acetate fraction of Biophytum sensitivum extract against sodium oxalate-induced urolithiasis in rats

Pawar, Anil T.; Vyawahare, N. S.

doi:10.1016/j.jtcme.2016.12.012

Cited by 15 publications

(11 citation statements)

References 35 publications

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“…Serum levels of urea, creatinine, and uric acid were significantly increased in the urolithiasic group (G2) compared to the untreated control group (G1), indicating renal damage ( Table 1 ). These results are consistent with those of a previous study and indicate that the accumulation of nitrogenous substances in the serum may be a consequence of a decreased glomerular filtration rate (GFR) due to lithiasic obstruction [ 23 ]. Uric acid binds to CaOx and modulates its crystallization and solubility and also reduces the inhibitory activity of glycosaminoglycans [ 20 ].…”

Section: Discussionsupporting

confidence: 92%

Antioxidant and Polyphenol-Rich Ethanolic Extract of Rubia tinctorum L. Prevents Urolithiasis in an Ethylene Glycol Experimental Model in Rats

et al. 2021

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Treatment of kidney stones is based on symptomatic medications which are associated with side effects such as gastrointestinal symptoms (e.g., nausea, vomiting) and hepatotoxicity. The search for effective plant extracts without the above side effects has demonstrated the involvement of antioxidants in the treatment of kidney stones. A local survey in Morocco has previously revealed the frequent use of Rubia tinctorum L. (RT) for the treatment of kidney stones. In this study, we first explored whether RT ethanolic (E-RT) and ethyl acetate (EA-RT) extracts of Rubia tinctorum L. could prevent the occurrence of urolithiasis in an experimental 0.75% ethylene glycol (EG) and 2% ammonium chloride (AC)-induced rat model. Secondly, we determined the potential antioxidant potency as well as the polyphenol composition of these extracts. An EG/AC regimen for 10 days induced the formation of bipyramid-shaped calcium oxalate crystals in the urine. Concomitantly, serum and urinary creatinine, urea, uric acid, phosphorus, calcium, sodium, potassium, and chloride were altered. The co-administration of both RT extracts prevented alterations in all these parameters. In the EG/AC-induced rat model, the antioxidants- and polyphenols-rich E-RT and EA-RT extracts significantly reduced the presence of calcium oxalate in the urine, and prevented serum and urinary biochemical alterations together with kidney tissue damage associated with urolithiasis. Moreover, we demonstrated that the beneficial preventive effects of E-RT co-administration were more pronounced than those obtained with EA-RT. The superiority of E-RT was associated with its more potent antioxidant effect, due to its high content in polyphenols.

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Section: Discussionsupporting

confidence: 92%

Antioxidant and Polyphenol-Rich Ethanolic Extract of Rubia tinctorum L. Prevents Urolithiasis in an Ethylene Glycol Experimental Model in Rats

et al. 2021

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“…A variety of animal models using rats were used to induce calcium oxalate urolithiasis. 16 Among these methods, the sodium oxalate simulated hyperoxaluria rat model triggers the rapid development of calcium oxalate stones in experimental renal tubules and is therefore commonly used for the rapid screening of antiurolithiatic drugs. 17 Intraperitoneal administration of sodium oxalate triggers hyperoxaluria in rats.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antiurolithiatic Potential of Piper cubeba Dried Fruits on Sodium Oxalate Induced Urolithiasis in Rats

Sura

Kumar²

2020

IJPI

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Objectives: The main endeavor of the work is to assess the antiurolithiatic potentials of ethanolic extract of Piper cubeba dried fruits (EEPC). Methods: EEPC was prepared using soxhlation, subjected to phytochemical screening and determined total phenolic and flavonoid content using Folin-Ciocalteu reagent and aluminium chloride colorimetric assay methods. In vitro antioxidant activity was evaluated using methods like 2,2-diphenyl-1picrylhydrazyl (DPPH) and lipid peroxidation assays using ascorbic acid as standard. Sodium oxalate (70 mg/kg, i.p) was given to rats for 5 days to promote urolithiasis. Cystone (750mg/kg, p. o), EEPC (100, 200 and 400 mg/kg, p.o) was administered to the respective group of rats from 6 th to 15 th day. On the 15 th day, serum and urine were collected from individual animals and biochemical parameters like BUN, creatinine and uric acid in serum and sodium, chloride, potassium, calcium, phosphate, oxalate in urine and calcium, phosphate and oxalate in kidney homogenate have been measured. The kidney sections have been prepared and histopathologically tested to check the stones. Results: Preliminary phytochemical analysis discloses the existence of phenolics, tannins, steroids, terpenoids and flavonoids. The EEPC enriched with phenols and flavonoids which correlates with its antioxidant potentials. Following treatment with cystone and EEPC, concentrations of BUN, creatinine, uric acid in serum and sodium, chloride, potassium, calcium, phosphate, oxalate in urine and calcium, phosphate, oxalate in kidney homogenate (P<0.001 vs. control) were significantly reduced in urolithiasis caused by sodium oxalate in a dose-dependent way. Conclusion: The promising results suggest that EEPC would act as a potential agent in the management of urolithiasis along with its antioxidant properties.

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“…Rats administered distilled water orally by gastric gavages (control group). NaOx group which intraperitoneally injected with 70mg sodium oxalate (NaOx) / kg b.wt for 7 days [20] and concurrently administered ACFE at three dosages (500,750 and 1000mg/kg b.wt) orally by gastric gavages.…”

Section: Experimental Design and Protocolmentioning

confidence: 99%

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2017

JCMAH

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The present study was performed to evaluate the antioxidative efficacy of Ananas comosus fruit extract (ACFE) against sodium oxalate (NaOx) induced oxidative damage in rats. Thirty rats were divided mainly into control group and NaOx group (70mg/kgb.wt, i.p.). The last one was subdivided into 4 subgroups; rats received vehicle (untreated NaOx), and ACFE at 500, 750, and 1000mg/kg b.wt respectively concurrently with NaOx for seven days. ACFE improved the serum and urine creatinine, urea, uric acid levels. Further, ACFE reduced the renal LPO concentration and enhance the levels of antioxidant enzymes. The present study concluded that the ameliorative effect of ACFE may be due to its chemical active substances such as phenols, flavonoids, alkaloids, saponins, glycosides, triterpenes, and tannins. However, the antioxidative ability of ACFE-derived bioactive compounds should be further investigated as a natural remedy for kidney diseases.

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Protective effect of ethyl acetate fraction of Biophytum sensitivum extract against sodium oxalate-induced urolithiasis in rats

Cited by 15 publications

References 35 publications

Antioxidant and Polyphenol-Rich Ethanolic Extract of Rubia tinctorum L. Prevents Urolithiasis in an Ethylene Glycol Experimental Model in Rats

Antioxidant and Polyphenol-Rich Ethanolic Extract of Rubia tinctorum L. Prevents Urolithiasis in an Ethylene Glycol Experimental Model in Rats

Evaluation of Antiurolithiatic Potential of Piper cubeba Dried Fruits on Sodium Oxalate Induced Urolithiasis in Rats

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