Effect of lactoferrin on Enteroaggregative<i>E. coli</i>(EAEC)This paper is one of a selection of papers published in this Special Issue, entitled 7th International Conference on Lactoferrin: Structure, Function, and Applications, and has undergone the Journal’s usual peer review process.

Ochoa, Theresa J.; Brown, Eric L.; Guion, Chase E.; Chen, Jane Z. ChenJ.Z.; McMahon, Robert J.; Cleary, Thomas G.

doi:10.1139/o06-053

Cited by 43 publications

(27 citation statements)

References 40 publications

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“…We have histidine tagged dispersin, a virulence protein involved in the aggregative adherence phenotype, and the surface fimbria (AAF-II). We found that lactoferrin-induced loss and degradation of AAF-II but not of dispersin [46].…”

Section: Enteroaggregative E Coli (Eaec)mentioning

confidence: 65%

“…Lactoferrin purified from human milk (Sigma-Aldrich Co. St. Louis, MO), recombinant human lactoferrin (Agennix, Houston, TX) and bovine lactoferrin (Tatua Cooperative Dairy Co, Morrinsville, New Zealand), inhibit the aggregative adherence "stacked-brick pattern" of EAEC in tissue cultured cells [45,46]. Bovine lactoferrin also inhibited EAEC biofilm formation and increased autoagglutination, further suggesting that surface adhesins were affected.…”

Section: Enteroaggregative E Coli (Eaec)mentioning

confidence: 99%

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Effect of lactoferrin on enteric pathogens

Ochoa

Cleary²

2009

Biochimie

118

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Much has been learned in recent years about the mechanisms by which breastfeeding improves child health and survival. However, there has been little progress in using these insights to improve pediatric care. Factors that are important for protecting the breast fed infant might be expected to decrease the adverse effects of weaning on diarrhea, growth, and development. Lactoferrin, an ironbinding protein with multiple physiological functions (anti-microbial, anti-inflammatory, and immunomodulatory), is one of the most important proteins present in mammalian milk. Protection against gastroenteritis is the most likely biologically relevant activity of lactoferrin. Multiple in vitro and animal studies have shown a protective effect of lactoferrin on infections with enteric microorganisms, including rotavirus, Giardia, Shigella, Salmonella and the diarrheagenic Escherichia coli. Lactoferrin has two major effects on enteric pathogens: it inhibits growth and it impairs function of surface expressed virulence factors thereby decreasing their ability to adhere or to invade mammalian cells. Thus, lactoferrin may protect infants from gastrointestinal infection by preventing the attachment by enteropathogens in the gut. Recently several clinical trials in children have started to address this issue. Whether lactoferrin can prevent a significant portion of diarrheal disease remains to be determined.

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Section: Enteroaggregative E Coli (Eaec)mentioning

confidence: 65%

Section: Enteroaggregative E Coli (Eaec)mentioning

confidence: 99%

Effect of lactoferrin on enteric pathogens

Ochoa

Cleary²

2009

Biochimie

118

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“…In contrast, apo-bLF increased the biofilm formation of S. mutans, while holo-bLF decreased the biofilm formation of S. mutans (18). bLF both with and without iron inhibited the biofilm formation of enteroaggregative E. coli (30). The inhibition of P. gingivalis and P. intermedia biofilm formation by LF was independent of the iron status of the protein, and the mechanism of action of LF against P. aeruginosa and S. mutans biofilm formation seems to be different from that against P. gingivalis and P. intermedia biofilm formation but similar to that against enteroaggregative E. coli biofilm formation.…”

Section: Vol 53 2009mentioning

confidence: 84%

“…Likewise, hLF at 0.13 mg/ml and apo-bLF, native bLF, and holo-bLF at 0.031 mg/ml inhibited P. intermedia biofilm formation. There are reports indicating that LF prevents the biofilm formation of Pseudomonas aeruginosa, Burkholderia cepacia, enteroaggregative Escherichia coli, and an oral pathogen, S. mutans (12,18,30,33,36). It has been documented that hLF inhibited the biofilm formation of P. aeruginosa by an iron-chelating mechanism (36).…”

Section: Vol 53 2009mentioning

confidence: 99%

Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation ofPorphyromonas gingivalisandPrevotella intermedia

Wakabayashi

Yamauchi

Kobayashi

et al. 2009

Antimicrob Agents Chemother

114

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Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with >130 g/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and >6 g/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (>8 g/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases.

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“…12) Moreover, lactoferrin has been shown to decrease the virulence of some enteric pathogens by decreasing their ability to adhere to mammalian cells or to invade them. 17) Antimicrobial activity has also been demonstrated for the peptides formed by the hydrolysis of lactoferrin with pepsin. These peptides have a wide spectrum of activity, this activity being stronger than that of native lactoferrin and being effective even in the presence of iron.…”

mentioning

confidence: 99%