Effect of Lactobacillus sporogenes (probiotic) on certain parasitological and molecular aspects in Schistosoma mansoni infected mice

Abstract

The prospective effects of Lactobacillus sporogenes (probiotics) and/or praziquantel (PZQ) treatment on some parasitological, histological and molecular aspects in Schistosoma mansoni infected mice were studied. The present data recorded that combination between PZQ (300 mg/Kg one dose 7 weeks post infection) and L. sporogenes (12.5 million spore/mice/week for 8 weeks from the first day of infection) reduced worm and ova count. Also, oogram patterns in liver and intestine recorded that treatment with L. sporogenes alone increase number of dead eggs especially in intestine. Histological observations showed significant (P \ 0.05) reduction in the mean values of granuloma diameters in liver and intestine in infected mice groups treated with PZQ and/or L. sporogenes. Single strand breaks (comet assay) showed increase in number of damaged and strong damaged lymphocyte cells in mice infected with S. mansoni and infected treated with PZQ while L. sporogenes administration reduced DNA damage. Flow cytometry also confirmed role of L. sporogenes in reducing significantly DNA damage according to determination of cell cycle analysis apoptosis. It can be concluded that administration of L. sporogenes accompanied with PZQ treatment ameliorates the hepatic and intestinal damage caused by S. mansoni infection.

Effect of probiotic acidophilus plus against infection with secondary hydatid disease in BALB /c mice

“…Moreover, bacterial output holds anthelmintic influence that might decrease maliciousness of numerous protozoa and helminthic illness (28). These results are similar to the study of Mohamed et al (29) and Zowail et al (30) who proved the protective and curative effects of useful bacteria in mice models against infection of Schistosoma mansoni (29)(30)(31). Many strains of probiotics, like Zymomonas mobilis 1*10 9 CFU/ml, when administrated by mouth provoked minor defense reaction, in mice against S. mansoni by providing prevention in treated animals (31,32).…”

“…Many strains of probiotics, like Zymomonas mobilis 1*10 9 CFU/ml, when administrated by mouth provoked minor defense reaction, in mice against S. mansoni by providing prevention in treated animals (31,32). When Lactobacillus sporogenes used orally at a certain dose, for a long time,since the beginning of the infestation with S. mansoni, minimized impairment of DNA, improves liver and gut deterioration, therefore weakened adult together with ova numbers (29).…”

Probiotics for the Control of Helminth Zoonosis

“…To date, various attempts have been made to investigate the protective and curative effects of beneficial bacteria in mice models for use in the control of S. mansoni [ 17 – 21 ]. Several probiotic strains, like Zymomonas mobilis , probiotic labneh containing Streptococcus salivarius subsp .…”

“…Their anthelmintic and immunomodulatory effects on S. mansoni are summarized in Table 1 . For instance, Lactobacillus sporogenes is among the most commonly studied [ 20 , 21 ] probiotic strains that showed a significant antischistosome effect in egg and larval stages of the parasite. It has remarkably reduced the worm burden as well as egg count.…”

“…For example, probiotic S. boulardii promoted a reduction in intensity of infection by T. canis by modulating cytokine mRNA expression, especially IL-12, in experimentally infected mice [ 46 ]. Furthermore, L. sporogenes act against cytokine induced apoptosis by decreased chromosomal aberrations and DNA damage in S. mansoni infected mice [ 20 , 21 ]. Nevertheless, modes of action of specific probiotics are generally not understood.…”