Effect of Lactobacillus plantarum FH185 on the Reduction of Adipocyte Size and Gut Microbial Changes in Mice with Diet-induced Obesity

Park, Sun Young; Cho, Seong A; Lee, Myung Ki; Lim, Sang Dong

doi:10.5851/kosfa.2015.35.2.171

Cited by 15 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results demonstrated that CFM and A29 treatment decreased adipose tissue size and mass in adipocytes and HFDinduced obese mice, respectively. The levels of fat deposition and size adipocytes were reduced after administration of L plantanrum Q180, 71 L plantanrum FH185 72 ; L plantanrum LMT1 73 ; L plantanrum LG42 74 ; L plantanrum KLDS1 36 ; L plantanrum No.14 75 ; and L plantanrum PS128 76 at different experimental models. All analyzed strains exert their effects at different ranges on the fat depositions in the 3T3-L1 and HFD-fed animal models.…”

Section: Discussionmentioning

confidence: 95%

“…Many reports evidenced that adipogenic major transcriptional factors such as PPAR-γ2, C/EBP-α, and SREBP-1 and genes involved in lipogenic pathway including FAS, aP2, and ACC were negatively regulated in adipocytes and obese mouse after treatment with L plantarum and its cell-free metabolites. 36,[72][73][74] Short-chain fatty acids (SCFA) secreted by probiotics exert an anti-obesity effect via the modulation of the lipid and glucose metabolism-associated markers, 85,86 resulting in reduced adipocyte size 75,87 ; Notably, SCFAs play a key in reducing fat deposits in adipocytes by accelerating fatty acid oxidation in HFD-fed mice and. 88 RT-PCR and western blot analysis results suggest that CFM and A29 significantly reduced fat deposition in adipocytes and HFDinduced obese mice via downregulating adipocyte-specific markers PPAR-γ, C/EBP-α, C/EBP-β, and SREBP-1.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

et al. 2020

View full text Add to dashboard Cite

Given the rising evidence that gut malfunction including changes in the gut microbiota composition, plays a major role in the development of obesity and associated metabolic diseases, the exploring of novel probiotic bacteria with potential health benefits has attracted great attention. Recently Lactobacillus spp., exert potent anti‐obesity effects by regulating key transcriptional and translational factors in adipose tissues. However, the molecular mechanism behind the anti‐obesity effect of probiotics is not yet fully understood. Therefore, we investigated the effect of Lactobacillus plantarum A29 on the expression of adipogenic and lipogenic genes in 3T3‐L1 adipocytes and high‐fat diet (HFD)‐fed mice. We observed that the treatment of 3T3‐L1 adipocytes with the cell‐free metabolites of L plantarum inhibited their differentiation and fat depositions via downregulating the key adipogenic transcriptional factors (PPAR‐γ, C/EBP‐α, and C/EBP‐β) and their downstream targets (FAS, aP2, ACC, and SREBP‐1). Interestingly, supplementation with L plantarum reduced the fat mass and serum lipid profile concurrently with downregulation of lipogenic gene expression in the adipocytes, resulting in reductions in the bodyweight of HFD‐fed obese mice. L plantarum treatment attenuated the development of obesity in HFD‐fed mice via the activation of p38MAPK, p44/42, and AMPK‐α by increasing their phosphorylation. Further analysis revealed that A29 modulated gut‐associated microbiota composition. Thus, A 29 potential probiotic strain may alleviate the obesity development and its associated metabolic disorders via inhibiting PPARγ through activating the p38MAPK and p44/42 signaling pathways.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In our previous study, L. plantarum FH185 was found to have lipase inhibitory activity of 70.09±2.04% and to inhibit the adipocyte differentiation of 3T3-L1 cells (18.63±0.98%) at a concentration of 100 μg/mL. It was also demonstrated that the strain has an effect on the reduction of adipocyte size and gut microbial changes in diet-induced obese mice ( Park et al ., 2015 ). The strain was incubated in a Lactobacilli MRS broth (Difco, USA) as the growth medium at 37℃ for 18 h.…”

Section: Methodsmentioning

confidence: 90%

Probiotic Characteristics of Lactobacillus plantarum FH185 Isolated from Human Feces

Park¹,

Lim²

2015

Korean Journal for Food Science of Animal Resources

Self Cite

View full text Add to dashboard Cite

Lactobacillus plantarum FH185 was isolated from the feces of healthy adults. In our previous study, L. plantarum FH185 was demonstrated that it has anti-obesity effect in the in vitro and in vivo test. In order to determine its potential for use as a probiotic, we investigated the physiological characteristics of L. plantarum FH185. The optimum growth temperature of L. plantarum FH185 was 40℃. L. plantarum FH185 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed higher resistance to polymyxin B and vancomycin. It also showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. Moreover, it was comparatively tolerant to bile juice and acid, and inhibited the growths of Salmonella Typhimurium and Staphylococcus aureus with rates of 44.76% and 53.88%, respectively. It also showed high adhesion activity to HT-29 cells compared to L. rhamnosus GG.

show abstract

“…Administration of Lab. plantarum to diet-induced obese mice induced reductions in adipocyte size and white adipose tissue weight, and anti-obesity effects including decreases in serum total cholesterol and leptin concentrations (Lee et al, 2018; Park et al, 2013a; Park et al, 2015; Takemura et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Probiotic BSH Activity and Anti-Obesity Potential of Lactobacillus plantarum Strain TCI378 Isolated from Korean Kimchi

Huang

Chen

et al. 2019

pnf

View full text Add to dashboard Cite

Lactobacillus (Lab.) is a human probiotic beneficial for the prevention and improvement of disease, yet properties of different Lab. strains are diverse. To obtain a Lab. strain that possesses greater potential against gastrointestinal dysfunction, we isolated Lactobacillus plantarum TCI378 (TCI378) from naturally fermented Korean kimchi. TCI378 has shown potential as probiotic since it can survive at pH 3.0 and in the presence of 0.3% bile acid. The bile salt hydrolase activity of TCI378 was shown by formation of opaque granular white colonies on solid de Man Rogosa Sharpe (MRS) medium supplemented with taurodeoxycholic acid, and its cholesterol-lowering ability in MRS medium supplemented with cholesterol. The metabolites of TCI378 from liquid culture in MRS medium prevented emulsification of bile salts. Moreover, both the metabolites of TCI378 and the dead bacteria reduced oil droplet accumulation in 3T3-L1, as detected by Oil red O staining. The expressions of adipocyte-specific genes perilipin 1 and glucose transporter type 4 were suppressed by the metabolites of TCI378, indicating TCI378 may have anti-obesity effects in adipocytes. These in vitro data show the potential of the prophylactic applications of TCI378 and its metabolites for reducing fat and lowering cholesterol.

show abstract

Effect of Lactobacillus plantarum FH185 on the Reduction of Adipocyte Size and Gut Microbial Changes in Mice with Diet-induced Obesity

Cited by 15 publications

References 33 publications

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

Probiotic Characteristics of Lactobacillus plantarum FH185 Isolated from Human Feces

Probiotic BSH Activity and Anti-Obesity Potential of Lactobacillus plantarum Strain TCI378 Isolated from Korean Kimchi

Contact Info

Product

Resources

About