Effect of Lactobacillus paracasei HII01 Supplementation on Total Cholesterol, and on the Parameters of Lipid and Carbohydrate Metabolism, Oxidative Stress, Inflammation and Digestion in Thai Hypercholesterolemic Subjects

Abstract

Hypercholesterolemia is one of the leading causes of cardiovascular disease. Probiotics can help to improve high blood lipid levels in hypercholesterolemia patients. Lactobacillus paracasei has been reported to have beneficial effects in several subjects; however, there is a lack of studies on Thai hypercholesterolemic subjects. Thus, this study was conducted in order to investigate the effect of L. paracasei HII01 on cholesterol, oxidative stress, and other biomarkers. Fifty-two subjects were randomized into two groups: the L. paracasei treatment group and the placebo group. The study was conducted over an intervention period of 12 weeks of supplementation. The results show that L. paracasei HII01 significantly reduced the total cholesterol (TCH), triglycerides (TGs), tumor necrosis factor (TNF)-α and lipopolysaccharide (LPS) of the patients, and increased their HDL, total antioxidant capacity (TAC) and propionic acid compared to the placebo group. Moreover, the supplementation of L. paracasei HII01 significantly increased lactic acid, IL-10 and IFN-γ, and substantially decreased malondialdehyde (MDA) at the end of the treatment. The results suggest that L. paracasei HII01 improves the blood lipid profile, reduces oxidative stress, and is beneficial for health among Thai hypercholesterolemic subjects.

Probiotics Supplementation Improves Intestinal Permeability, Obesity Index and Metabolic Biomarkers in Elderly Thai Subjects: A Randomized Controlled Trial

“…The subjects in the probiotic group were provided with aluminum foil sachets containing a mixture of probiotics (2.0 × 10 10 CFU of Lactobacillus paracasei HII01; 2.0 × 10 10 CFU of Bifidobacterium breve; 1.0 × 10 10 CFU of Bifidobacterium longum), which was received from Lactomason Co., Ltd., Jinju-si, South Korea, and the placebo group were provided with 10 g of corn starch in a similar package of probiotics. The instructions for the consumption of the supplement were detailed in our previous report [18].…”

“…The subjects' personal history was assessed, and their demographic characteristics were detailed. The body mass index (BMI) and weight of the subjects were measured using an electronic scale (Picooc ® , Model S1 Pro, Beijing, China) [18].…”

“…The biochemical parameters such as blood urea nitrogen (BUN), creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C), triglycerides (TG), and fasting blood sugar The subjects' personal history was assessed, and their demographic characteristics were detailed. The body mass index (BMI) and weight of the subjects were measured using an electronic scale (Picooc ® , Model S1 Pro, Beijing, China) [18].…”

“…Urine samples were collected from subjects to determine intestinal permeability. The samples were analyzed as detailed in our previous study [18] using a colorimetric commercial kit (EnzyChrom™, BioAssay, Hayward, CA, USA). Other biomarkers in the urine, such as quinolinic acid (QA) and 5-hydroxyindoleacetic acid (5-HIAA), were determined using ELISA commercial kit (Fivephoton Biochemicals™, San Diego, CA, USA for QA, and Immusmol, Bordeaux, France for 5-HIAA).…”

“…Probiotics are the most recognized method to improve gut microbiota and treat dysbiosis [12][13][14]. The studies reported that the probiotics had enhanced the homeostasis of intestinal permeability [15], reduced inflammation [16], and also improved several ill-health conditions in humans [17,18]. However, the mechanism behind the beneficial effect of probiotics on health benefits is not explored.…”