Heat‐Killed Lactobacillus rhamnosusATCC 7469 Improved UVB‐Induced Photoaging ViaAntiwrinkle and Antimelanogenesis Impacts

Abstract

Exposure of ultraviolet B (UVB) radiation is the main factor from the environment to cause skin photoaging. Lactobacillus rhamnosus ATCC 7469, is a probiotic strain with a good track record for enhancing human health. The present study conducted the impacts of heat‐killed L. rhamnosus ATCC 7469 (RL) on photoaging in vitro using mouse skin fibroblast (MSF) cells and human epidermal melanocytes (HEM) exposed to UVB. The results showed that (1) RL‐protected UVB‐induced cytotoxicity relating to absorb UVB and reduce DNA damage. (2) RL exerted the antiwrinkle impact involved in two aspects. Firstly, RL downregulated MMP‐1, 2, 3 expressions associating with MAPK signaling, resulting in the increased the protein expression of COL1A1, further booting type I collagen abundant thereby promoting the antiwrinkle impact in MSF cells. Secondly, RL reduced ROS content, further decreasing oxidative damage relating to Nrf2/Sirt3/SOD2 signaling, thereby promoting the antiwrinkle impact in MSF cells. (3) RL suppressed tyrosinase and TYRP‐2 activity and/or levels associating with PKA/CREB/MITF signaling, thereby promoting antimelanogenesis impact in HEM cells. In conclusion, our findings suggest that RL could reduce photoaging caused by UVB via antiwrinkle and antimelanogenesis properties and may be a potential antiphotoaging beneficial component, which is applied in the cosmetic industry.

METTL14 affects UVB‐induced human dermal fibroblasts photoaging via miR‐100‐3p biogenesis in an m6A‐dependent manner

“…Previous studies have proposed multiple schemes for constructing photoaging cellular models. In this investigation, human dermal fibroblasts (HDFs) were induced using a 30 mJ/cm 2 dose of UVB irradiation to construct a photoaging cellular model that mimics real‐life conditions and is experimentally convenient (Kang et al., 2020 ; Kim et al., 2019 ; Oh et al., 2018 ; Wang et al., 2022 ; Zhang et al., 2023 ).…”

Effects of Bacterial Lysates and Metabolites on Collagen Homeostasis in TNF-α-Challenged Human Dermal Fibroblasts

“…Several studies have evaluated the type I pro-collagen production and/or MMP-1 synthesis by HDFs upon exposure to different forms of bacteria, in search of antiaging benefits of bacteria via the gut–skin axis. For instance, the effects of heat-killed bacteria ( Lactobacillus acidophilus KCCM12625P [ 27 ], Lactiplantibacillus plantarum HY7714 [ 28 ], Lacticaseibacillus paracasei MCC1849 [ 29 ], and Lacticaseibacillus rhamnosus ATCC 7469 [ 30 ]) or bacterial components and metabolites (lipoteichoic acid of Latilactobacillus sakei KCCM 11175P [ 31 ], exopolysaccharide of L. plantarum HY7714 [ 32 ], and cell-free supernatants of Bifidobacterium animalis ssp. lactis MG741 [ 33 ] and several Lactobacillus , Streptococcus , and Bifidobacterium strains [ 34 ]) have been explored.…”

Applications of Probiotic Constituents in Cosmetics

“…Through an experimental study, Xiaofang Zhang et al [16] discovered the anti-wrinkle effect of heat-killed Lactobacillus rhamnosus (RL). After receiving RL, mouse skin fibroblasts' antioxidant capacity was increased and their ROS content was decreased, and human epidermal melanocytes were also able to produce anti-melanin, which might be used as an anti-photoaging ingredient in cosmetics.…”