Effect of Lactobacillus johnsonii La1 and antioxidants on intestinal flora and bacterial translocation in rats with experimental cirrhosis

Chiva, M.; Soriano, Germán; Rochat, Isabelle; Peralta, Carmen; Rochat, Florence; Llovet, Teresa; Mirelis, Beatriz; Schiffrin, Eduardo J.; Guarner, Carlos; Balanzó, Joaquím

doi:10.1016/s0168-8278(02)00142-3

Cited by 126 publications

(86 citation statements)

References 43 publications

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“…The most important of these mechanisms are intestinal colonization resistance, intestinal mucosal barrier, and body reticuloendothelial system function (Wells et al, 1987). Previous studies showed that these mechanisms are severely impaired in some serious diseases, such as acute liver injury, cirrhosis, severe scalding injury and hemorrhagic shock, which could lead to excessive growth of gram negative aerobic bacteria, bacteria and endotoxin translocation, and aggravated liver injury (Li et al, 2004;Chiva et al, 2002;Kasravi et al, 1997;Wang et al, 2002). But data on the relationship between intestinal microflora status and ischemia/reperfusion (I/R) liver injury are not available.…”

Section: Introductionmentioning

confidence: 99%

Intestinal microflora in rats with ischemia/reperfusion liver injury

Xing

Li²,

Xu³

et al. 2005

J Zheijang Univ Sci B

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Abstract:Objectives: To investigate the intestinal microflora status related to ischemia/reperfusion (I/R) liver injury and explore the possible mechanism. Methods: Specific pathogen free grade Sprague-Dawley rats were randomized into three groups: Control group (n=8), sham group (n=6) and I/R group (n=10). Rats in the control group did not receive any treatment, rats in the I/R group were subjected to 20 min of liver ischemia, and rats in the sham group were only subjected to sham operation. Twenty-two hours later, the rats were sacrificed and liver enzymes and malondialdehyde (MDA), superoxide dismutase (SOD), serum endotoxin, intestinal bacterial counts, intestinal mucosal histology, bacterial translocation to mesenteric lymph nodes, liver, spleen, and kidney were studied. Results: Ischemia/reperfusion increased liver enzymes, MDA, decreased SOD, and was associated with plasma endotoxin elevation in the I/R group campared to those in the sham group. Intestinal Bifidobacteria and Lactobacilli decreased and intestinal Enterobacterium and Enterococcus, bacterial translocation to kidney increased in the I/R group compared to the sham group. Intestinal microvilli were lost, disrupted and the interspace between cells became wider in the I/R group. Conclusion: I/R liver injury may lead to disturbance of intestinal microflora and impairment of intestinal mucosal barrier function, which contributes to endotoxemia and bacterial translocation to kidney.

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Section: Introductionmentioning

confidence: 99%

Intestinal microflora in rats with ischemia/reperfusion liver injury

Xing

Li²,

Xu³

et al. 2005

J Zheijang Univ Sci B

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“…It has been proposed that intestinal oxidative stress plays an important role in the BT process by disrupting the intestinal barrier function, and several studies have reported that antioxidant treatments reduce BT by preventing intestinal oxidative injury (6,20,21). It has also been reported that steroids prevent intestinal oxidative damage (9,10).…”

Section: Discussionmentioning

confidence: 99%

Methylprednisolone prevents bacterial translocation in thioacetamide-induced liver failure in rats

Harputluoğlu

Temel²,

Demirel³

et al. 2017

Turk J Gastroenterol

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“…According to our results, Mutaflor administered for eight days had no effect on the above-mentioned parameters. Chiva et al (2002) tested the influence of the probiotic Lactobacillus johnsonii La1 in combination with antioxidants on BT using the tetrachloromethane model of liver cirrhosis in laboratory rats. These authors concluded that it was possible to achieve a decrease in BT into lymph nodes using the combination of Lactobacillus johnsonii La1 and antioxidants under specific conditions.…”

Section: Discussionmentioning

confidence: 99%

Influence of the Escherichia coli Nissle 1917 strain on complications of the chronic experimental liver damage

Kosáková¹,

Scheer²,

Lata³

et al. 2007

Vet. Med.

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ABSTRACT:The aim of the study was evaluate the influence of the probiotic Escherichia coli Nissle 1917 strain (Mutaflor ® suspension, Ardeypharm GmbH, Herdecke, Germany) on bacterial translocation in cases of liver damage, damage to the intestinal mucosa, potential portal hypertension associated with possible development of oesophageal varices and on the bacterial population of the intestine during chronic experimental liver damage in the laboratory rat. Rats with liver damage induced by thioacetamide were divided into an experimental and control group. Experimental and control animals were applied Mutaflor and saline, respectively. Samples of blood, liver, lymph nodes and caecum for microbiological examination, of liver, duodenum and oesophagus for histological examination and of spleen for weight evaluation were collected. There were no significant differences between both groups of animals in the qualitative proportion of Staphylococcus spp., Enterococcus spp. and Proteus spp. cultured from the lymph nodes, blood and liver. The quantitative culture results on Enterococcus spp. in the caecum, liver and lymph nodes showed no significant differences between both groups. There was a significant difference between the experimental and control group in the counts of coliform bacteria. No significant differences between both groups were found in the overall damage score of the liver, duodenum and oesophagus. There were no differences in the spleen to body weight ratio of both groups. The application of Mutaflor ® suspension for eight days had no recognisable effect diminishing the selected complications of chronic liver damage caused by the administration of TAA to laboratory rats.

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Effect of Lactobacillus johnsonii La1 and antioxidants on intestinal flora and bacterial translocation in rats with experimental cirrhosis

Cited by 126 publications

References 43 publications

Intestinal microflora in rats with ischemia/reperfusion liver injury

Intestinal microflora in rats with ischemia/reperfusion liver injury

Methylprednisolone prevents bacterial translocation in thioacetamide-induced liver failure in rats

Influence of the Escherichia coli Nissle 1917 strain on complications of the chronic experimental liver damage

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