Effect of laboratory‐isolated Lactobacillus plantarum LGFCP4 from gastrointestinal tract of guinea fowl on growth performance, carcass traits, intestinal histomorphometry and gastrointestinal microflora population in broiler chicken

Abstract

The study aimed to investigate the effect of feed supplements, viz Lactobacillus plantarum LGFCP4 (laboratory isolate from GIT of Guinea fowl), Lactobacillus acidophilus (NCDC, Karnal) and in-feed antibiotic bacitracin methylene disalicylate (BMD) on growth performance, FCR, carcass traits and immune organs weight, intestinal histomorphometry and gastrointestinal microflora population in broiler chickens. In a completely randomized design, CARIBRO-Dhanraja broiler chicks (n = 160) were used with four treatment groups. During the entire experimental duration of 35 days, treatment groups were provided with different dietary treatments (T1 - basal diet (negative control), T2 - antibiotic growth promoter BMD 20 g/100 kg feed (positive control), T3 - 1 × 10 cfu of L. acidophilus/gm-fermented feed +MOS 1 g/kg feed and T4 - 1 × 10 cfu of laboratory-isolated L. plantarum LGFCP4/gm-fermented feed+ MOS 1 g/kg feed. After 35 days of experimental period, no significant results have been observed in different growth performance traits among treatment groups. Cut-up parts and edible organs' weight remained unaffected by dietary supplementation, whereas weight of immune organs were significantly higher (p < 0.05) in L. plantarum LGFCP4-supplemented group. At the end of feeding trial, significantly (p < 0.05) lower E. coli count was observed in crop of T4 birds, while in ileum, T2 and T3 showed lower count. In caeca, T2 group showed lowest E. coli count. Salmonella count in crop and ileum was significantly (p < 0.05) low in T3 and T4, while in caeca, T2 group showed lowest count. In terms of histomorphometry, duodenal villous height (VH), crypt depth (CD) and VH:CD ratio were higher for T3 and T4 and lowest values were obtained for T2 group. The results of the study showed that L. plantarum LGFCP4 isolated from GIT of guinea fowl can effectively replace in-feed antibiotic growth promoters in broiler diets by altering intestinal villi morphology and improving the gut health by reducing the pathogenic microbial load.

Comparative Effects of Using New Multi-Strain Synbiotics on Chicken Growth Performance, Hematology, Serum Biochemistry and Immunity

“…Production parameters are often used for the evaluation of the influence of synbiotics and probiotics on the health of broiler chickens. Many experimental models have shown that these nutritional supplements usually improve the performance of broiler chickens, but the results depend on the type of synbiotic used [1,10,11,13,15,16,18,21,[32][33][34][35][36][37][38][39][40][41][42][43]. Usually, these parameters are measured at the end of the production cycle but, to improve the quality of analysis, we decided to measure them at several time points.…”

“…According to the currently most accepted definition, probiotics are single-or multi-component preparations containing live microorganisms that, when administered in appropriate numbers, bring health benefits to the host [12]. Probiotics improve the microbiological balance of intestines and strengthen the intestinal barrier against the influence of harmful factors [13]. Prebiotics are preparations containing indigestible nutrients that favorably affect the growth and/or activity of one or more intestinal bacterial species [14,15].…”

Xylanase and Fermented Polysaccharide of Hericium caputmedusae Reduce Pathogenic Infection of Broilers by Improving Antioxidant and Anti‐Inflammatory Properties

“…Meanwhile, the probiotics can affect the expression of genes associated with fatty acid synthesis and oxidation [38]. L. plantarum can effectively replace in-feed antibiotic and improve the intestinal health by changing intestinal villus morphology and inhibiting the pathogenic loading [39]. Lactobacillus species effectively absorb and expel heavy metal toxicity from the gastrointestinal tract of broilers [40].…”

Simultaneous Supplementation of Bacillus subtilis and Antibiotic Growth Promoters by Stages Improved Intestinal Function of Pullets by Altering Gut Microbiota

“…Gut microbiota alteration could influence the intestinal morphology (Louis and Flint, 2009 ; Jahanian et al, 2017 ; Vineetha et al, 2017 ). A negative correlation between the population of caecal B. fragilis and ileal crypt depth was identified by further Pearson correlation analysis.…”