Effect of laboratory isolated <i>Lactobacillus plantarum</i> LGFCP4 on growth performance, intestinal histomorphometry and gastrointestinal microflora population in Guinea fowl

Vineetha, P.G.; Tomar, SS; Saxena, V.K.; Arun, Tülin; Adil, K.

doi:10.5958/0974-8180.2018.00015.6

Cited by 4 publications

(9 citation statements)

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“…The performance of chickens is often used for the evaluation of the influence of synbiotics (probiotics/prebiotics) on broiler chickens health. In many experimental models, it has been shown that these nutritional supplements usually improve the performance of broiler chickens, but the results depend on type of synbiotics used [37][38][39][40][41][42][43][44][45][46][47] . Usually parameters of the performance are measured at the end of the production cycle, but for improved analysis we decided to check it at each time point.…”

Section: Discussionmentioning

confidence: 99%

The effect of synbiotic preparations on the intestinal microbiota and her metabolism in broiler chickens

Śliżewska

Markowiak‐Kopeć

Żbikowski

et al. 2020

Sci Rep

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The aim of the research was to determine the effect of newly elaborated synbiotic preparations on the count of dominant intestinal microorganisms, on the profile of fatty acids (short chain -SCFA and branched chain -BCFA), the lactic acid produced and the performance of chickens. The studies determined the composition of the dominant intestinal microbiota with use of the culture method. The fatty acid profile was also determined using the high-performance liquid chromatography method (HPLC). Moreover, the performance of chickens was determined such as the daily cumulative mortality rate, the feed conversion ratio (FCR) and the European Production Efficiency Factor (EPEF). It was found that synbiotics had a beneficial effect on parameters of the performance of chickens, and also resulted in increase in the count of beneficial bacteria and to the restriction in growth of potential pathogens in the gastrointestinal tract. Synbiotics caused an increase in the concentration of lactic acid and SCFA and a decrease in the concentration of BCFA in the broiler's excreta. These results showed a beneficial effect of the tested synbiotics on the intestinal microbiota, their metabolism and the performance of broiler chickens. The elaborated synbiotics can be successfully used as feed additives for broiler chickens.

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Section: Discussionmentioning

confidence: 99%

The effect of synbiotic preparations on the intestinal microbiota and her metabolism in broiler chickens

Śliżewska

Markowiak‐Kopeć

Żbikowski

et al. 2020

Sci Rep

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“…Six of the seven trials with one or more outcomes assessed for bias reported the use of random allocation to treatment group (Jamroz et al ., 2003; Olnood et al ., 2007; Baurhoo et al ., 2009; Amerah et al ., 2012; Bostami et al ., 2016; Vineetha et al ., 2017), although none provided information on the method used to generate the random sequence. For the remaining domains of bias, none of the trials provided the information necessary to evaluate the potential risks of bias, and therefore there were ‘some concerns’ about the potential risk of bias for all bias domains for all outcomes.…”

Section: Resultsmentioning

confidence: 99%

“…The most commonly reported outcome was FCR, which was evaluated in seven trials with a total 25 treatment comparisons with at least one eligible antibiotic (Jamroz et al ., 2003; Olnood et al ., 2007; Baurhoo et al ., 2009; Viveros et al ., 2011; Amerah et al ., 2012; Bostami et al ., 2016; Vineetha et al ., 2017) (Table 3). Results were presented at the arm level for all of the trials and thus the effect sizes shown in Table 3 were calculated post hoc .…”

Section: Resultsmentioning

confidence: 99%

“…The majority of studies (n = 8/9) were conducted in a single flock. Seven trials reported FCR (Jamroz et al, 2003;Olnood et al, 2007;Baurhoo et al, 2009;Viveros et al, 2011;Amerah et al, 2012;Bostami et al, 2016;Vineetha et al, 2017), and one trial also reported mortality (Amerah et al, 2012). Two trials reported assessment of one or more relevant outcomes, but the data were not presented in a form that could be extracted (Cracknell et al, 1986;Huff et al, 2004).…”

Section: Study Characteristicsmentioning

confidence: 99%

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The efficacy of antibiotics to control colibacillosis in broiler poultry: a systematic review

Sargeant

Bergevin

Churchill

et al. 2019

Anim. Health. Res. Rev.

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The objective of this systematic review was to evaluate the efficacy of antibiotics to prevent or control colibacillosis in broilers. Studies found eligible were conducted controlled trials in broilers that evaluated an antibiotic intervention, with at least one of the following outcomes: mortality, feed conversion ratio (FCR), condemnations at slaughter, or total antibiotic use. Four electronic databases plus the gray literature were searched. Abstracts were screened for eligibility and data were extracted from eligible trials. Risk of bias was evaluated.Seven trials reported eligible outcomes in a format that allowed data extraction; all reported results for FCR and one also reported mortality. Due to the heterogeneity in the interventions and outcomes evaluated, it was not feasible to conduct meta-analysis.Qualitatively, for FCR, comparisons between an antibiotic and an alternative product did not show a significant benefit for either. Some of the comparisons between an antibiotic and a no-treatment placebo showed a numerical benefit to antibiotics, but with wide confidence intervals. The risk-of-bias assessment revealed concerns with reporting of key trial features.The results of this review do not provide compelling evidence for or against the efficacy of antibiotics for the control of colibacillosis.

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“…Many Limosilactobacillus species have "Qualified Presumption of Safety (QPS)" status from the European Food Safety Authority (EFSA) and "Generally Regarded As Safe (GRAS)" status from US Food and Drug Administration (FDA). Lactobacilli are key members of the endogenous microbiota of oral, GI, respiratory, and urogenital mucosa and play a key role in regulating local microbiota, restoring barrier function, preventing inflammation associated with GI diseases, improving growth performance, and protecting against infectious diseases (Chen et al, 2021;Han et al, 2021;Hu et al, 2021;Klaenhammer et al, 2002;Li et al, 2018;Manes-Lazaro et al, 2017;Ouwehand et al, 2002;Siddique et al, 2021;Vineetha et al, 2017; Y. Wu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Recombinant Limosilactobacillus (Lactobacillus) delivering nanobodies against Clostridium perfringens NetB and alpha toxin confers potential protection from necrotic enteritis

Gangaiah

Ryan

Hoesel³

et al. 2022

MicrobiologyOpen

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Necrotic enteritis (NE), caused by Clostridium perfringens , is an intestinal disease with devastating economic losses to the poultry industry. NE is a complex disease and predisposing factors that compromise gut integrity are required to facilitate C. perfringens proliferation and toxin production. NE is also characterized by drastic shifts in gut microbiota; C. perfringens is negatively correlated with Lactobacilli. Vaccines are only partially effective against NE and antibiotics suffer from the concern of resistance development. These strategies address only some aspects of NE pathogenesis. Thus, there is an urgent need for alternative strategies that address multiple aspects of NE biology. Here, we developed Limosilactobacillus ( Lactobacillus ) reuteri vectors for in situ delivery of nanobodies against NetB and α toxin, two key toxins associated with NE pathophysiology. We generated nanobodies and showed that these nanobodies neutralize NetB and α toxin. We selected L. reuteri vector strains with intrinsic benefits and demonstrated that these strains inhibit C. perfringens and secrete over 130 metabolites, some of which play a key role in maintaining gut health. Recombinant L. reuteri strains efficiently secreted nanobodies and these nanobodies neutralized NetB. The recombinant strains were genetically and phenotypically stable over 480 generations and showed persistent colonization in chickens. A two‐dose in ovo and drinking water administration of recombinant L. reuteri strains protected chickens from NE‐associated mortality. These results provide proof‐of‐concept data for using L. reuteri as a live vector for delivery of nanobodies with broad applicability to other targets and highlight the potential synergistic effects of vector strains and nanobodies for addressing complex diseases such as NE.

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Effect of laboratory isolated Lactobacillus plantarum LGFCP4 on growth performance, intestinal histomorphometry and gastrointestinal microflora population in Guinea fowl

Cited by 4 publications

References 0 publications

The effect of synbiotic preparations on the intestinal microbiota and her metabolism in broiler chickens

The effect of synbiotic preparations on the intestinal microbiota and her metabolism in broiler chickens

The efficacy of antibiotics to control colibacillosis in broiler poultry: a systematic review

Recombinant Limosilactobacillus (Lactobacillus) delivering nanobodies against Clostridium perfringens NetB and alpha toxin confers potential protection from necrotic enteritis

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