Effect of L-Ascorbic Acid on the Climbing Ability and Protein Levels in the Brain of<i>Drosophila</i>Model of Parkinson's Disease

Khan, Safiya; Jyoti, Smita; Naz, Falaq; Shakya, Barkha; Rahul, .; Afzal, Mohammad; Siddique, Yasir Hasan

doi:10.3109/00207454.2012.709893

Cited by 36 publications

(20 citation statements)

References 23 publications

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“…Our earlier studies with nordihydroguaiaretic acid [51], curcumin [52], capsaicin [53], and ascorbic acid [54] also showed a dose-dependent delay in the loss of climbing ability in the same PD model flies. The study with ascorbic acid, a well known antioxidant that showed no significant difference in the protein levels in the brain of PD model fly, suggests that only free radical scavenging activity is involved in the protection against the PD symptoms [54]. The decrease in lipid peroxidation and apoptosis in the brain of PD model flies may be due to the inhibitory effect of the ACNC on α S aggregation or due to the scavenging of free radicals resulting from the oxidative stress.…”

Section: Discussionmentioning

confidence: 95%

“…The efficacy of levodopa declines as PD progresses, but the oxidative stress can be reduced by agents having the free radical scavenging potential [50]. Our earlier studies with nordihydroguaiaretic acid [51], curcumin [52], capsaicin [53], and ascorbic acid [54] also showed a dose-dependent delay in the loss of climbing ability in the same PD model flies. The study with ascorbic acid, a well known antioxidant that showed no significant difference in the protein levels in the brain of PD model fly, suggests that only free radical scavenging activity is involved in the protection against the PD symptoms [54].…”

Section: Discussionmentioning

confidence: 99%

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Synthesis of Alginate-Curcumin Nanocomposite and Its Protective Role in TransgenicDrosophilaModel of Parkinson’s Disease

et al. 2013

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The genetic models in Drosophila provide a platform to understand the mechanism associated with degenerative diseases. The model for Parkinson's disease (PD) based on normal human alpha-synuclein (αS) expression was used in the present study. The aggregation of αS in brain leads to the formation of Lewy bodies and selective loss of dopaminergic neurons due to oxidative stress. Polyphenols generally have the reduced oral bioavailability, increased metabolic turnover, and lower permeability through the blood brain barrier. In the present study, the effect of synthesized alginate-curcumin nanocomposite was studied on the climbing ability of the PD model flies, lipid peroxidation, and apoptosis in the brain of PD model flies. The alginate-curcumin nanocomposite at final doses of 10−5, 10−3, and 10−1 g/mL was supplemented with diet, and the flies were allowed to feed for 24 days. A significant dose-dependent delay in the loss of climbing ability and reduction in the oxidative stress and apoptosis in the brain of PD model flies were observed. The results suggest that alginate-curcumin nanocomposite is potent in delaying the climbing disability of PD model flies and also reduced the oxidative stress as well as apoptosis in the brain of PD model flies.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Synthesis of Alginate-Curcumin Nanocomposite and Its Protective Role in TransgenicDrosophilaModel of Parkinson’s Disease

et al. 2013

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“…(). However, ascorbic acid on its own my also affect the climbing ability of PINK1 flies (Khan et al ., ); therefore, flasks to which only ascorbic acid was added were used as an additional control. The negative control contained no herbs or chemicals.…”

Section: Methodsmentioning

confidence: 99%

Effects of Five Ayurvedic Herbs on Locomotor Behaviour in a Drosophila melanogaster Parkinson's Disease Model

Jansen

Brogan

Whitworth

et al. 2014

Phytotherapy Research

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Current conventional treatments for Parkinson's disease (PD) are aimed at symptom management, as there is currently no known cure or treatment that can slow down its progression. Ayurveda, the ancient medical system of India, uses a combination of herbs to combat the disease. Herbs commonly used for this purpose are Zandopa (containing Mucuna pruriens), Withania somnifera, Centella asiatica, Sida cordifolia and Bacopa monnieri. In this study, these herbs were tested for their potential ability to improve climbing ability of a fruit fly (Drosophila melanogaster) PD model based on loss of function of phosphatase and tensin-induced putative kinase 1 (PINK1). Fruit flies were cultured on food containing individual herbs or herbal formulations, a combination of all five herbs, levodopa (positive control) or no treatment (negative control). Tests were performed in both PINK1 mutant flies and healthy wild-type (WT) flies. A significant improvement in climbing ability was observed in flies treated with B. monnieri compared with untreated PINK1 mutant flies. However, a significant decrease in climbing ability was observed in WT flies for the same herb. Centella asiatica also significantly decreased climbing ability in WT flies. No significant effects were observed with any of the other herbs in either PINK1 or WT flies compared with untreated flies.

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“…Although the removal of the extracellular GLU protects neurons from excitotoxicity caused by the activation of the membrane GLU receptors (Rebec et al, ), a persistent activation of AA–GLU heteroexchange decreases the intracellular AA, thereby limiting its antioxidant action (Harrison and May, 2009) and promoting the reactive oxygen species (ROS) actions on dopaminergic cells (Wagner et al, ). Ascorbate ameliorates motor deficits and dopaminergic cell degeneration in Drosophila (Khan et al, ) and rat (Naoi and Maruyama, ) models of PD. Thus, the idea of a possible delay effect of AA on PD progression in humans has been circulating for a long time (Fahn, ).…”

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Ascorbate prevents cell death from prolonged exposure to glutamate in an in vitro model of human dopaminergic neurons

Ballaz

Moráles

Rodrı́guez

et al. 2013

J of Neuroscience Research

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Ascorbate (vitamin C) is a nonenzymatic antioxidant highly concentrated in the brain. In addition to mediating redox balance, ascorbate is linked to glutamate neurotransmission in the striatum, where it renders neuroprotection against excessive glutamate stimulation. Oxidative stress and glutamatergic overactivity are key biochemical features accompanying the loss of dopaminergic neurons in the substantia nigra that characterizes Parkinson's disease (PD). At present, it is not clear whether antiglutamate agents and ascorbate might be neuroprotective agents for PD. Thus, we tested whether ascorbate can prevent cell death from prolonged exposure to glutamate using dopaminergic neurons of human origin. To this purpose, dopamine-like neurons were obtained by differentiation of SH-SY5Y cells and then cultured for 4 days without antioxidant (antiaging) protection to evaluate glutamate toxicity and ascorbate protection as a model system of potential factors contributing to dopaminergic neuron death in PD. Glutamate dose dependently induced toxicity in dopaminergic cells largely by the stimulation of AMPA and metabotropic receptors and to a lesser extent by N-methyl-D-aspartate and kainate receptors. At relatively physiological levels of extracellular concentration, ascorbate protected cells against glutamate excitotoxicity. This neuroprotection apparently relies on the inhibition of oxidative stress, because ascorbate prevented the pro-oxidant action of the scavenging molecule quercetin, which occurred over the course of prolonged exposure, as is also seen with glutamate. Our findings show the relevance of ascorbate as a neuroprotective agent and emphasize an often underappreciated role of oxidative stress in glutamate excitotoxicity. Occurrence of a glutamate-ascorbate link in dopaminergic neurons may explain previous contradictions regarding their putative role in PD.

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Effect of L-Ascorbic Acid on the Climbing Ability and Protein Levels in the Brain ofDrosophilaModel of Parkinson's Disease

Cited by 36 publications

References 23 publications

Synthesis of Alginate-Curcumin Nanocomposite and Its Protective Role in TransgenicDrosophilaModel of Parkinson’s Disease

Synthesis of Alginate-Curcumin Nanocomposite and Its Protective Role in TransgenicDrosophilaModel of Parkinson’s Disease

Effects of Five Ayurvedic Herbs on Locomotor Behaviour in a Drosophila melanogaster Parkinson's Disease Model

Ascorbate prevents cell death from prolonged exposure to glutamate in an in vitro model of human dopaminergic neurons

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