Effect of ketoprofen on immune cells in mice

Hamdani, Dawood Ahmad; Javeed, Aqeel; Ashraf, Muhammad Imran; Nazir, Jawad; Ghafoor, Aamir

doi:10.4314/tjpr.v13i11.6

Cited by 3 publications

(4 citation statements)

References 13 publications

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“…The results of the present study clearly show that intramammary KET diminishes the increase of SCC in response to LPS. Previous studies in rats and mice showed that systemic and local administration of KET reduces the influx of immune cells such as leukocytes and macrophages to the pleura and peritoneum through an unknown mechanism (Vannier et al, 1989;Hamdani et al, 2015). A selective inhibition of the IL-8-induced chemotaxis of human intestinal neutrophils in vitro by KET has been reported by Bizzarri et al (2001).…”

Section: Discussionmentioning

confidence: 92%

Ketoprofen affects the mammary immune response in dairy cows in vivo and in vitro

Dan

Bruckmaier

Wellnitz

2018

Journal of Dairy Science

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Nonsteroidal anti-inflammatory drugs are commonly administered parenterally in addition to antimicrobial mastitis therapy to increase the well-being of the diseased animal. As mastitis is usually a localized infection of mammary tissue, we tested the hypothesis that a local administration of nonsteroidal anti-inflammatory drugs through the teat canal could have anti-inflammatory effects on the affected area. We investigated the effects of intramammarily administered ketoprofen (KET) during an LPS-induced immune response on somatic cell count (SCC) and blood-milk barrier integrity. In addition, we investigated the effects of KET on the mRNA abundance of immune factors and their prostaglandin E2 secretion in primary bovine mammary epithelial cells in vitro. Six cows received 0.2 µg of LPS (serotype O26:B6) together with 50 mg of KET into one quarter and LPS only in the opposing quarter. The increase of SCC and of serum albumin (SA) and IgG concentrations and the increase of lactate dehydrogenase (LDH) activity in milk induced by LPS were lower in quarters that received KET in addition. In 3 cows, intramammary KET (50 mg) without additional LPS did not affect SCC, SA, IgG, and LDH in milk. Effects of KET on the immune response of mammary epithelial cells in vitro were investigated in cells from 3 cows challenged with or without LPS (0.2 µg/mL) and with or without additional KET in 2 concentrations (1.25 or 2.5 mg/mL). Ketoprofen reduced the LPS-induced increase of mRNA abundance of tumor necrosis factor α, IL-8, serum amyloid A, and cyclooxygenase-2. The mRNA abundance of cyclooxygenase-1 and prostaglandin E synthase was reduced in cells without LPS challenge by addition of KET at 2.5 mg/mL. Furthermore, the LPS-induced secretion of prostaglandin E2 of mammary epithelial cells into the supernatant could not be detected if KET was added. The results demonstrate that intramammary KET diminishes the increase of SCC and reduces the impairment of the blood-milk barrier (based on SA and LDH in milk), leading to a reduced IgG concentration in milk during LPS-induced mastitis. In mammary epithelial cells, KET limits the expression of several immune factors that are increased during an immune response. In summary, intramammary administration of KET reduces the inflammatory response in the mammary gland. However, it remains unclear whether the inhibited transfer of immune cells and IgG from blood into milk after KET administration would reduce the success of the immune defense in infectious mastitis.

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Section: Discussionmentioning

confidence: 92%

Ketoprofen affects the mammary immune response in dairy cows in vivo and in vitro

Dan

Bruckmaier

Wellnitz

2018

Journal of Dairy Science

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“…[ 17 ] Ketoprofen is another NSAID, which is known to suppress the T-cell-mediated DTH reaction. [ 18 ]…”

Section: Discussionmentioning

confidence: 99%

“…It was suggested that NSAIDs enhance cell apoptosis and reduce granulocyte colony stimulating factor which plays an important role in the development of new neutrophils. [ 18 ]…”

Section: Discussionmentioning

confidence: 99%

“…In the current study, all the doses of mefenamic acid were found to decrease antibody titer in a dose-dependent manner as compared with control group, similar to the effect of cyclophosphamide used as a reference drug. Hamdaniet al[ 18 ] suggested that ketoprofen treated mice decreased the production of IgG and IgM antibody against sheep RBCs. The decreased antibody titer found in our study might also be due to the decrease production of IgG and IgM antibodies in the serum of mefenamic acid-treated mice.…”

Section: Discussionmentioning

confidence: 99%

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Immunomodulatory activity of mefenamic acid in mice models of cell-mediated and humoral immunity

et al. 2016

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Objectives:Previously, different nonsteroidal anti-inflammatory drugs (NSAIDs) have been evaluated for their potential immunomodulatory activities. Mefenamic acid is a well-known NSAID and is used in the treatment of musculoskeletal disorders, inflammation, fever, and pain. To the best of our knowledge, promising data regarding the immunomodulatory activity of mefenamic acid is scarce. Current study investigates the immunomodulatory activity of mefenamic acid in different models of cell-mediated and humoral immunity.Materials and Methods:Immunomodulatory effects on cell-mediated immunity were evaluated using dinitrochlorobenzene-induced delayed type hypersensitivity (DTH) and cyclophosphamide-induce myelosuppression assays. While effects on humoral immunity were evaluated using hemagglutination assay and mice lethality test.Results:Hematological analysis showed that mefenamic acid significantly reduced white blood cell count, red blood cell (RBC) count, hemoglobin content, lymphocytes levels, and neutrophils levels in healthy mice as compared with control, suggesting the immunosuppressive activity of mefenamic acid. Treatment with mefenamic acid also significantly reduced all the hematological parameters in cyclophosphamide-induced neutropenic mice, as compared with positive control group. We found that treatment with mefenamic acid significantly suppressed DTH after 24 h, 48 h, and 72 h, as compared with positive control group. Mefenamic acid treated groups showed a significant reduction in antibody titer against sheep RBCs as compared to control group, similar to the effect of cyclophosphamide. We also found increased mice lethality rate in mefenamic acid treated groups, as compared with positive control group.Conclusions:The results provided basic information of immunosuppression of mefenamic acid on both cell-mediated and humoral immunity.

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