Immunomodulatory activity of mefenamic acid in mice models of cell-mediated and humoral immunity

Shabbir, Arham; Arshad, Hafiza Maida; Shahzad, Muhammad; Shamsi, Sadia; Ashraf, Muhammad Imran

doi:10.4103/0253-7613.178837

Cited by 6 publications

(8 citation statements)

References 16 publications

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“…A dose of 2 mg/kg, administered intramuscularly 45 min prior to, or at the time of, surgical mulesing had no effect on pain-related behaviours, cortisol or β-endorphin response [ 344 ], although when administered intravenously, 2 mg/kg tolfenamic acid can reduce the mechanical nociceptive threshold in sheep [ 353 ]. Mefenamic acid has not been studied in sheep, and a single article on intraperitoneal administration in laboratory mice indicated that it had suppressive effects on both the cell-mediated and humoral immune systems [ 187 ].…”

Section: Resultsmentioning

confidence: 99%

Analgesia for Sheep in Commercial Production: Where to Next?

Small

Fisher

et al. 2021

Animals

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Increasing societal and customer pressure to provide animals with ‘a life worth living’ continues to apply pressure on livestock production industries to alleviate pain associated with husbandry practices, injury and illness. Over the past 15–20 years, there has been considerable research effort to understand and develop mitigation strategies for painful husbandry procedures in sheep, leading to the successful launch of analgesic approaches specific to sheep in a number of countries. However, even with multi-modal approaches to analgesia, using both local anaesthetic and non-steroidal anti-inflammatory drugs (NSAID), pain is not obliterated, and the challenge of pain mitigation and phasing out of painful husbandry practices remains. It is timely to review and reflect on progress to date in order to strategically focus on the most important challenges, and the avenues which offer the greatest potential to be incorporated into industry practice in a process of continuous improvement. A structured, systematic literature search was carried out, incorporating peer-reviewed scientific literature in the period 2000–2019. An enormous volume of research is underway, testament to the fact that we have not solved the pain and analgesia challenge for any species, including our own. This review has highlighted a number of potential areas for further research.

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Section: Resultsmentioning

confidence: 99%

Analgesia for Sheep in Commercial Production: Where to Next?

Small

Fisher

et al. 2021

Animals

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“…Many medicinal mushrooms are used to treat various specific health problems [13,16]. As can be seen in Table 2, the extract of T. clypeatus showed activity on the other test microorganisms (2.5-10.5 mm).…”

Section: Discussionmentioning

confidence: 96%

“…Fresh cow red blood cells (CRBC) were obtained from cow's blood as described by Heden (1946) [12] and used as antigen [13]. Briefly, veinous blood was collected from cow in EDTA-tubes and kept in the laboratory for 15 min.…”

Section: Red Blood Cellsmentioning

confidence: 99%

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Assessment of antimicrobial and immunomodulatory activities of termite associated fungi, Termitomyces clypeatus R. Heim (Lyophyllaceae, Basidiomycota)

et al. 2018

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Background: Termitomyces clypeatus (T. clypeatus) is an edible mushroom specie which grows in various areas in Cameroon. It is indicated by several healers in treatment of several infectionsand is frequently used for this purpose. However, no study has been reported on its antibacterial and immunomodulatory activities which were the aim of this study. Methods: Disc diffusion method was used to appreciate the bactericidal activity against 4 bacteria and 2 yeast species. The immunomodulatory activities were assessed in mice, where the extract was administered by gavage and as supplement in the feed. The treatment was done for 10 days. Delayed type hypersensitivity test was carried out to assess the cell mediated immune response while the effect on humoral immunity was evaluated using hemagglutination assay and mice lethality test.. The body weight was also recorded in mice used for delayed hypersensitivity. Results: It was found that extract of T. clypeatus highly inhibited the growth of bacteria and yeast at different ratios compared to the medium (P < 0.05). The extract of T. clypeatus has reversed the immunosuppressed effects of dexamethasone on antibody formation. In addition, it significantly decreased of the mice lethality rate in mice infected by Pasteurella multocida (P < 0.05). Administration of T. clypeatus also significantly increased the delayed type hypersensitivity response in healthy and dexamethasone immunosuppressed mice (P < 0.05), but it significantly reduced the body weight of mice after 10 days. Conclusion: The results provided basic information demonstrating the antibacterial activity and immunostimulatory activity of T. clypeatus on both cell-mediated and humoral immunity.

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“…Arthritis Group 5 was an anti-inflammatory reference group and received piroxicam (10 mg/kg b.w., i.p.) [ 34 ]. All rats were euthanized using ketamine and xylazine on day 28 [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Preclinical Rodent Models of Arthritis and Acute Inflammation Indicate Immunomodulatory and Anti-Inflammatory Properties of Juglans regia Extracts

Mobashar

Shabbir

Shahzad

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Juglans regia has been used to treat inflammatory and arthritic disorders in traditional medicine. The present study aimed to investigate the antiarthritic and anti-inflammatory potential of ethanolic leaves extract of J. regia. Arthritis was induced in rodents with Freund’s complete adjuvant. J. regia treatment was started on 8th day of arthritis induction and sustained for 20 days. Acute inflammatory models were developed using carrageenan, histamine, serotonin, and dextran. Qualitative and GC-MS analyses were also performed. Arthritis was determined using an arthritis scoring index and histopathological examination of ankle joints. RT-PCR was performed to determine the expression of pro-inflammatory markers (TNF-α, NF-κB, IL-6, IL-1β, and COX-2) and anti-inflammatory IL-4. PGE2 levels were evaluated using an ELISA. Blood and biochemical parameters were also determined. Paw edema was measured using a digital plethysmometer. Treatment with extracts inhibited arthritic development and attenuated paw edema along with all histopathological parameters. The expression levels of pro-inflammatory cytokines and COX-2 were downregulated, while IL-4 was upregulated. PGE2 levels were also reduced in extract-treated groups. Blood and biochemical parameters were nearly normalized in the treatment groups. Both extracts significantly inhibited carrageenan, histamine, serotonin, and dextran-induced paw edema. Qualitative phytochemical screening and GC-MS analysis confirmed that extracts possessed potential medicinal compounds. In conclusion, ethanol and n-hexane extracts of J. regia leaves have immunomodulatory and anti-inflammatory effects that ameliorate experimentally induced arthritis and edema. The inhibition of autacoids may also be one of the mechanisms inducing the immunomodulatory effect.

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Immunomodulatory activity of mefenamic acid in mice models of cell-mediated and humoral immunity

Cited by 6 publications

References 16 publications

Analgesia for Sheep in Commercial Production: Where to Next?

Analgesia for Sheep in Commercial Production: Where to Next?

Assessment of antimicrobial and immunomodulatory activities of termite associated fungi, Termitomyces clypeatus R. Heim (Lyophyllaceae, Basidiomycota)

Preclinical Rodent Models of Arthritis and Acute Inflammation Indicate Immunomodulatory and Anti-Inflammatory Properties of Juglans regia Extracts

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