Effect of KC-404 on experimental cerebral infarction in rats.

Kudo, Yoshitaka; Ichikawa, Yoshimitsu; Sugiura, Hiroyuki; Nishino, Koichiro; Irikura, Tsutomu

doi:10.1254/fpj.85.435

Cited by 5 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Platelet aggregation rates and plasma levels of TXB2 and 6-keto-PGF,a increased after the glass bead injection; however, after pretreatment with indomethacin, all of these measurements were significantly reduced (Figs. 6,7).…”

Section: Resultsmentioning

confidence: 99%

Cerebral Microcirculatory Changes After Cerebral Embolization Induced by Glass Bead Injection in Rabbits

Nagano¹,

Suzuki²,

Hayashi³

et al. 1992

Angiology

View full text Add to dashboard Cite

The authors investigated microcirculatory changes in cerebral embolization induced by an injection of glass beads (mesh size 20/400, 15 mg/body) using intravital microscopy and reflectance spectrophotometry in normal rabbits. Cerebral embolization with the glass bead injection reduced the inner diameter of cerebral arterioles, the index of cerebral hemoglobin concentration (IHb), and the index of oxygen saturation (ISO2). Moreover, platelet aggregation rates and plasma levels of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) appreciably increased. During observation of the pial vasculature, "white bodies" became visible at the entrance of arteriolar branchings two to three minutes after the glass bead injection. After indomethacin administration (3 mg/kg, IV), they could not observe white bodies, and platelet aggregation rates were significantly reduced. The plasma levels of TXB2 and 6-keto-PGF1 alpha were also significantly reduced. These findings suggest that glass beads do injury to the microvascular endothelial cells leading to development of white bodies, ie, "flying thrombi." Therefore, the cerebral microvascular embolization induced by the glass bead injection appears to be an experimental model for evaluating efficacy of remedies for the cerebral microcirculatory disorders.

show abstract

Section: Resultsmentioning

confidence: 99%

Cerebral Microcirculatory Changes After Cerebral Embolization Induced by Glass Bead Injection in Rabbits

Nagano¹,

Suzuki²,

Hayashi³

et al. 1992

Angiology

View full text Add to dashboard Cite

show abstract

“…A variety of animal models of cerebral ischemia have been developed in rats (1)(2)(3)(4). The development of the 4-vessel occlusion method (1) provided new applications for using rats as a model of bilateral hemispheric ischemia.…”

mentioning

confidence: 99%

Effects of pentobarbital and cyproheptadine on brain ischemia induced by bilateral occlusions of carotid arteries and vertebral arteries of second cervical vertebra in rats.

et al. 1988

View full text Add to dashboard Cite

Abstract-The bilateral hemispheric ischemia in rats was induced by the occlusion of bilateral common carotid arteries after permanent electrocauterization of bilateral vertebral arteries at the level of the second cervical vertebra.In ischemic rats, (a) electroencephalograms became flat immediately after occlusion of carotid arteries, and (b) mortalities reached maximum levels at day 3 after recirculation.These results suggested that a constant level of cerebral ischemia was produced in this rat model. Pentobarbital markedly inhibited the mortality in these ischemic rats, whereas cyproheptadine did not.A variety of animal models of cerebral ischemia have been developed in rats (1-4). The development of the 4-vessel occlusion method (1) provided new applications for using rats as a model of bilateral hemispheric ischemia. This model has gained popularity because of the relative ease of preparation and has been used in a variety of experimental studies. However, the level of ischemia ob tained by this method is not constant, because the invisible vertebral arteries of the first cervical vertebra (C1) are electrocauterized, and therefore, it is difficult to confirm the completeness of the electrocauterization (3, 4). The inconsistent results make this model unsuitable for the screening of compounds for therapeutic effects on cerebral infarction. We have found that the vertebral arteries at the level of the second cervical vertebra (C2) are visible in Sprague-Dawley rats and can be completely electrocauterized without fail ure. The vertebral arteries at the level of the second cervical vertebra in rats of the Lewis and F-344 strains were also observed at the same place. This paper reports a method for producing reversible occlusion of the carotid arteries after permanent interruption of the vertebral arteries at the level of C2 in unanes thetized rats and changes in electroence phalograms (EEGs) and mortality in ischemic rats with 4-vessel occlusion. By using this model, we investigated the effects of pen tobarbital and cyproheptadine on the cerebral ischemia in rats.Male rats of the Sprague-Dawley strain, weighing 330-380 g, were used throughout the experiments.The animals were main tained on laboratory food and tap water ad libitum under a well-controlled environment. They were anesthetized with sodium pen tobarbital (50 mg/kg, i.p.) and the vertebral arteries of the second cervical vertebra were exposed carefully after separating the para spiral muscles.The vertebral arteries run through the foramina of C2 and travel beneath the alar foramina of C1. An electro cautery needle was inserted through each foramen of C2 and both vertebral arteries were permanently electrocauterized (Fig. 1 A). Thus, complete occlusion of the vertebral arteries was confirmed by direct observation in all of the operated rats. Immediately after the elec trocauterization, the site of incision was closed carefully. The common carotid arteries were exposed bilaterally through a midline cervical incision and isolated their nerve trunks. The s...

show abstract

Effects of ibudilast on hippocampal long-term potentiation and passive avoidance responses in rats with transient cerebral ischemia

Yoshioka

Suda

Mori

et al. 2002

Pharmacological Research

View full text Add to dashboard Cite

Effect of KC-404 on experimental cerebral infarction in rats.

Cited by 5 publications

References 0 publications

Cerebral Microcirculatory Changes After Cerebral Embolization Induced by Glass Bead Injection in Rabbits

Cerebral Microcirculatory Changes After Cerebral Embolization Induced by Glass Bead Injection in Rabbits

Effects of pentobarbital and cyproheptadine on brain ischemia induced by bilateral occlusions of carotid arteries and vertebral arteries of second cervical vertebra in rats.

Effects of ibudilast on hippocampal long-term potentiation and passive avoidance responses in rats with transient cerebral ischemia

Contact Info

Product

Resources

About