Effect of Juice and Extracts from Saposhnikovia divaricata Root on the Colon Cancer Cells Caco-2

Abstract

Colorectal cancer ranks 3rd in terms of cancer incidence. Growth and development of colon cancer cells may be affected by juice and extracts from Saposhnikovia divaricata root. The objective of the research was to analyze the effect of S. divaricata juice and extracts on the viability, membrane integrity and types of cell death of Caco-2 cells. Juice and extracts were analyzed using Ultra-High Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS) and in respect of the presence of antioxidants, total carbohydrates, protein, fat and polyphenols. The contents of cimifugin β-D-glucopyranoside, cimifugin, 4′-O-glucopyranosyl-5-O-methylvisamminol, imperatorin and protein were the highest in juice. 50% Hydroethanolic extract had the greatest antioxidant potential, concentration of polyphenols and fat. Water extract was characterized by the highest content of glutathione. Juice and 75% hydroethanolic extract contained the most carbohydrates. After the application of juice, 50% extract and the juice fraction containing the molecules with molecular weights >50 kDa, a decrease of the cell viability was noted. Juice and this extract exhibited the protective properties in relation to the cell membranes and they induced apoptosis. The knowledge of further mechanisms of anticancer activity of the examined products will allow to consider their use as part of combination therapy.

Comprehensive studies on the properties of apple juice treated by non-thermal atmospheric plasma in a flow-through system

“…The human intestinal Caco-2 cell line model was extensively used over past few years to screen bioavailability of elements ions from various food products and beverages 10 , 34 . Here, the cytotoxic effect of NTAP-treated AJ on human colon adenocarcinoma cells (Caco-2) and normal human intestinal epithelial cells (FHs 74 Int) were studied by using the MTT assay in order to reveal any cytotoxic properties of NTAP-treated AJ.…”

“…Cytotoxic properties of the NTAP-treated apple juice. The human intestinal Caco-2 cell line model was extensively used over past few years to screen bioavailability of elements ions from various food products and beverages 10,34 . Here, the cytotoxic effect of NTAP-treated AJ on human colon adenocarcinoma cells (Caco-2) Table 5.…”

Calcium Carbonate Nanoparticles—Toxicity and Effect of In Ovo Inoculation on Chicken Embryo Development, Broiler Performance and Bone Status

“…The levels of glucose (Glu), total protein (TP), albumin (Alb), total cholesterol (TC), and triglyceride (TG) were determined. Glutathione (GSH) was measured quantitively using Ellman’s method modified by Matusiewicz et al [ 31 ]; in this method, 5,5ʹ-dithiobis(2-nitrobenzoic acid) (DTNB, Ellman’s reagent) is reduced by thiol compounds to form a colored product (2-nitro-5-mercaptobenzoic acid) with the maximum absorbance at 412 nm. To 375 µL of serum samples from each group, 19.7 µL of 50% trichloroacetic acid (TCA) was added and centrifuged (1200× g , 5 min).…”

Effect of Elaeagnus umbellata (Thunb.) fruit extract on H2O2-induced oxidative and inflammatory responses in normal fibroblast cells

“…The anti-oxidant in vitro properties of extracts were measured using DPPH free radical scavenging test, as previously shown by Matusiewicz et al (2009) . Concentrations ranging from 100 to 1,000 µg/mL were used and the results were expressed as a percentage of DPPH inhibition.…”