Effect of jasmonic acid and chitosan on triterpenoid production in Calendula officinalis hairy root cultures

Alsoufi, Abdulwadood Shakir Mahmood; Pączkowski, Cezary; Szakiel, Anna; Długosz, Marek

doi:10.1016/j.phytol.2019.02.030

Cited by 48 publications

(56 citation statements)

References 23 publications

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“…The compounds were identified by their chromatographic mobility, mass spectra, and retention times, and quantified by an external standard method, as described in chapter 4.5. As reported in our previous papers [12,17], different C. officinalis hairy root lines can vary slightly in their sterol profile of (particularly with regard to the presence or absence of isofucosterol) and can accumulate intermediates of their biosynthetic pathways (e.g., cycloartanol and 24-methylenecycloartanol). However, in all basic, non-elicited lines, the predominant compounds remain the same, i.e.…”

Section: Introductionmentioning

confidence: 53%

“…Jasmonic acid was found to be a very effective elicitor, increasing both the accumulation of oleanolic acid saponins in the hairy root tissue and their secretion to the medium with a parallel decrease in the biosynthesis and accumulation of sterols. Chitosan slightly increased the accumulation and secretion of oleanolic acid saponins and increased the total content of sterols [12]. In the present work, we studied the effect of selected abiotic factors, i.e.…”

Section: Introductionmentioning

confidence: 97%

“…stigmasterol as the principal compound followed by sitosterol, as detected in natural C. officinalis roots [17]. Nevertheless, some alterations in the ratio among individual compounds were observed after the elicitation of hairy roots with jasmonic acid and chitosan [12], and thus such phenomena can be expected after treatment with other elicitors and different stress factors.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we reported the influence of selected biotic elicitors, jasmonic acid and chitosan, on the biosynthesis, accumulation, and secretion of triterpenoids in two lines of Calendula officinalis in vitro hairy root culture, obtained as a result of transformation with the wild type Agrobacterium rhizogenes strain ATCC 15834 [12]. C. officinalis hairy roots produce triterpenoid saponins (oleanolic acid glycosides) in varied yields and excrete these compounds into the culture medium [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Influence of Selected Abiotic Factors on Triterpenoid Biosynthesis and Saponin Secretion in Marigold (<em>Calendula officinalis L.</em>) in vitro Hairy Root Cultures

Alsoufi¹,

Pączkowski²,

Długosz³

et al. 2019

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The aim of the study was the evaluation of the efficiency of selected abiotic elicitors, i.e. silver and cadmium ions, ultrasound, and UV-C irradiation, in the stimulation of triterpenoid biosynthesis, accumulation, and saponin secretion in Calendula officinalis hairy root cultures. Apart from the possible enhancement of triterpenoid production, the relationship between primary and secondary metabolism (represented respectively by sterols and pentacyclic triterpenes), modifications of the sterol compositional profile, and fluctuations in the total triterpenoid content were monitored in the performed experiments. The main phenomenon observed as a response to heavy metal treatment was the stimulation (up to 12-fold) of the secretion of saponins, accompanied by significant changes in sterol composition. Ultrasound stimulated the secretion of saponins (up to 11-fold); however, it exerted diverse influences on the triterpenoid content in hairy root tissue (stimulating or decreasing) depending on the duration of the exposure to the elicitor. UV-C radiation caused a slight increase in the content of both sterols and saponins in hairy root tissue, and stimulated saponin secretion up to 8.5-fold. The expected symptoms of the competition between the biosynthetic pathways of sterols and pentacyclic triterpenoids were less evident in reactions to abiotic stressors than those reported previously for biotic elicitors

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Section: Introductionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Selected Abiotic Factors on Triterpenoid Biosynthesis and Saponin Secretion in Marigold (<em>Calendula officinalis L.</em>) in vitro Hairy Root Cultures

Alsoufi¹,

Pączkowski²,

Długosz³

et al. 2019

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“…In P. ginseng hairy root cultures, the three phytohormones, 2,4-dichlorophenoxyacetic acid (2,4-D), NAA, and indolebutyric acid (IBA), improved the growth of the hairy roots and promoted saponin accumulation to different degrees, and 0.5 mg/L IBA significantly promoted the accumulation of total saponins and ginsenoside Rb1 [24]. In Calendula officinalis in vitro hairy root culture, jasmonic acid (JA) treatment increased both the accumulation of oleanolic acid saponins in the hairy root tissue (up to 20-fold) and, in particular, the secretion of these compounds to the medium (up to 113-fold) [25]. In addition, in previous studies we found that application of 1.0 mg/L IBA alone and in combination with 1.0 mg/L 6-BA was advantageous to growth and increased total oleanolic acid and ecdysterone contents in A. bidentata roots.…”

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NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl

et al. 2020

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Application of plant growth regulators has become one of the most important means of improving yield and quality of medicinal plants. To understand the molecular basis of phytohormone-regulated oleanolic acid metabolism, RNA-seq was used to analyze global gene expression in Achyranthes bidentata treated with 2.0 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L 6-benzyladenine (6-BA). Compared with untreated controls, the expression levels of 20,896 genes were significantly altered with phytohormone treatment. We found that 13071 (62.5%) unigenes were up-regulated, and a lot of differentially expressed genes involved in hormone or terpenoid biosynthesis, or transcription factors were significantly upregulated. These results suggest that oleanolic acid biosynthesis induced by NAA and 6-BA occurs due to the expression of key genes involved in jasmonic acid signal transduction. This study is the first to analyze the production and hormonal regulation of medicinal A. bidentata metabolites at the molecular level. The results herein contribute to a better understanding of the regulation of oleanane-type triterpenoid saponins accumulation and define strategies to improve the yield of these useful metabolites. OPEN ACCESSCitation: Liu Y, Tang L, Wang C, Li J (2020) NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl. PLoS ONE 15(2): e0229490. https://doi.org/10.triterpenoid biosynthetic pathway can be divided into three steps: synthesis of universal terpenoid precursors, formation of carbon skeletons, and modification of triterpenoid skeletons. Triterpenoid precursors are synthesized mainly through the cytoplasmic mevalonate (MVA) pathway. The plastidic 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway is believed to be a minor pathway for triterpenoid biosynthesis [17]. Triterpenoid skeleton synthesis genes include FPS (farnesyl diphosphate synthase), SS (squalene synthase), SE (squalene epoxidase), and OSCs (oxidosqualene cyclases). The cyclization of 2,3-oxidosqualene catalyzed by OSCs is a key step in the biosynthesis of triterpenoid saponins and sterols. β-amyrin synthase (β-AS) and cycloartenol synthase (CAS) catalyze the conversion of 2,3-oxidosqualene to β-amyrin and cycloartenol, which are precursors for oleanolic acid and phytosterols, respectively. A lot is known about several genes involved in modification of the triterpenoid skeleton downstream of the cyclization step are known. Some cytochrome P450 monooxygenases (CYP450s) and glycosyl transferases (GTs) were shown to catalyze modifications of triterpenoid skeletons, including hydroxylation and glycosidation. An increasing number of studies have indicated that the CYP716 family belongs to the CYP85 clan of CYP450s, and is involved in biosynthesis of various triterpenoids including oleanane backbones [18,19]. For example, CYP716A12 is involved in oxidation of the β-amyrin skeleton, which modifies the oleanane backbone [20]. CYP716A52v2 catalyzes the conversion of βamyrin to oleanolic acid in P. ginseng [21]. Howeve...

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In Vitro Production of Saponins

Naik

Sudheer

Dubey

et al. 2022

Nutraceuticals Production From Plant Cell Factory

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Effect of jasmonic acid and chitosan on triterpenoid production in Calendula officinalis hairy root cultures

Cited by 48 publications

References 23 publications

Influence of Selected Abiotic Factors on Triterpenoid Biosynthesis and Saponin Secretion in Marigold (<em>Calendula officinalis L.</em>) in vitro Hairy Root Cultures

Influence of Selected Abiotic Factors on Triterpenoid Biosynthesis and Saponin Secretion in Marigold (<em>Calendula officinalis L.</em>) in vitro Hairy Root Cultures

NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl

In Vitro Production of Saponins

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