Effect of ischaemia on the enzyme activity of the hepatic tissue

Kempler, Péter; Stekker, K; Németh, M

doi:10.1007/bf01851436

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…A standardized surgical model was used for assessment of liver IR damage as described previously [17], [18], [19]. (Figure 1) Briefly, after median laparotomy and mobilization of the liver, lobes III, IV, V were subjected to 60 min ischemia by clamping of the biliovascular trunk using an atraumatic microvascular clip.…”

Section: Methodsmentioning

confidence: 99%

Levosimendan: A Cardiovascular Drug to Prevent Liver Ischemia-Reperfusion Injury?

et al. 2013

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IntroductionTemporary occlusion of the hepatoduodenal ligament leads to an ischemic-reperfusion (IR) injury in the liver. Levosimendan is a new positive inotropic drug, which induces preconditioning-like adaptive mechanisms due to opening of mitochondrial KATP channels. The aim of this study was to examine possible protective effects of levosimendan in a rat model of hepatic IR injury.Material and MethodsLevosimendan was administered to male Wistar rats 1 hour (early pretreatment) or 24 hours (late pretreatment) before induction of 60-minute segmental liver ischemia. Microcirculation of the liver was monitored by laser Doppler flowmeter. After 24 hours of reperfusion, liver and blood samples were taken for histology, immuno- and enzyme-histochemistry (TUNEL; PARP; NADH-TR) as well as for laboratory tests. Furthermore, liver antioxidant status was assessed and HSP72 expression was measured.ResultsIn both groups pretreated with levosimendan, significantly better hepatic microcirculation was observed compared to respective IR control groups. Similarly, histological damage was also reduced after levosimendan administration. This observation was supported by significantly lower activities of serum ALT (pearly = 0.02; plate = 0.005), AST (pearly = 0.02; plate = 0.004) and less DNA damage by TUNEL test (pearly = 0.05; plate = 0.034) and PAR positivity (pearly = 0.02; plate = 0.04). Levosimendan pretreatment resulted in significant improvement of liver redox homeostasis. Further, significantly better mitochondrial function was detected in animals receiving late pretreatment. Finally, HSP72 expression was increased by IR injury, but it was not affected by levosimendan pretreatment.ConclusionLevosimendan pretreatment can be hepatoprotective and it could be useful before extensive liver resection.

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Section: Methodsmentioning

confidence: 99%

Levosimendan: A Cardiovascular Drug to Prevent Liver Ischemia-Reperfusion Injury?

et al. 2013

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“…4 A Klinika kísérleti műtőjében 1965 óta folytak olyan állatkísérletek, amelynek célja a máj konzerválása, illetve bizonyosan a beültetés gyakorlása volt. [5][6][7] A Klinika több munkatársa tett látogatást külföldi transzplantációs munkacsoportoknál (Roy Calne, Cardiff; Rudolf Pichlmayr, Hannover; Thomas E. Starzl, Pitts burgh). Az első magyarországi májátültetés 1983. február 1-én történt.…”

Section: A Modern Korunclassified

Májsebészet a Semmelweis Egyetem I. sz. Sebészeti Klinikáján

Hahn

Kempler

Pekli

et al. 2020

MaSeb

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Untersuchungen zur Morphologie und Biochemie der Wundheilung bei Leberschnittverletzungen

et al. 1980

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The early phase of wound repair after liver incisions was investigated by histological, enzyme-histochemical, and biochemical methods in guinea pigs. There are considerable differences in cellular and histochemical reactions in comparison to incisions of the skin: 1. The rapid leukocytic reaction showed little change during the observation interval (48 h). In short survival times epithelial cells show signs of hypoxidosis and loss of glycogen at the edge of an incision. Proliferation of organ-specific and mesenchymal cells was observed after more than 24 h. 2. Alterations of the investigated enzymes in the traumatic area are important diagnostic parameters in the early stages of wound healing. 3. In the early 12 h after injury distinct increase of histamine was observed, while serotonin was noticed to decrease. 4. Therefore, the determination of wound age in the early wound reactions after injuries of the liver should include enzymehistochemical methods.

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Effect of ischaemia on the enzyme activity of the hepatic tissue

Cited by 4 publications

References 23 publications

Levosimendan: A Cardiovascular Drug to Prevent Liver Ischemia-Reperfusion Injury?

Levosimendan: A Cardiovascular Drug to Prevent Liver Ischemia-Reperfusion Injury?

Májsebészet a Semmelweis Egyetem I. sz. Sebészeti Klinikáján

Untersuchungen zur Morphologie und Biochemie der Wundheilung bei Leberschnittverletzungen

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