Water is an essential resource for food production, as agriculture consumes close to 70% of the 15 total freshwater, and its shortage is becoming critical in arid and semiarid areas of the world. 16 Therefore, it is important to use water more efficiently. The objectives of this project are to 17 determine the productive response and the irrigation water use efficiency of seedless watermelon to 18 three irrigation management strategies over two growing seasons. This was done by applying 100, 19 75 and 50% of the irrigation water requirements (IWR) the first year, in the second year added six 20 additional treatments, of which three treatments were regulated deficit irrigation with 75% IWR 21 during the vegetative growth, fruit development and fruit ripening stages, and the other three 22 treatments were with 50% IWR during the same stages. The exposure of watermelon plants to 23 severe deficit irrigation resulted in a reduction in dry biomass, total and marketable yield, average 24 fruit weight, fruit number and harvest index, and without improvement of marketable fruit quality. 25 The fruit ripening was the less sensitive stage to water deficits. Relative water content and cell 26 membrane stability index decreased as the water deficit increased. Irrigation water use efficiency 27 decreased to a lesser extend during the fruit ripening stage than when water restriction were 28 applied during different growth stages. If water is readily available, irrigating with 100% of water 29 requirements is recommended, but in the case of water scarcity, applying water shortage during fruit ripening stage would be advisable.