Effect of iron sources on the glycosylation macroheterogeneity of human recombinant IFN-γ produced by CHO cells during batch processes

Clincke, Marie-Françoise; Guédon, Emmanuel; Yen, Frances T; Ogier, Virginie; Goergen, Jean‐Louis

doi:10.1186/1753-6561-5-s8-p114

Cited by 8 publications

(9 citation statements)

References 3 publications

(4 reference statements)

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“…143 One possible alternative to the use of growth factors is the small molecule antioxidant chelator aurintricarboxylic acid (ATA), which has been shown to promote CHO cell growth by acting on IGF-1 receptors (in combination with lysophosphatidic acid) in a manner similar to that of insulin. ATA could therefore Iron Impacts glycosylation macroheterogeneity of product 224 ; Supports cell growth and health 125…”

Section: Growth Factorsmentioning

confidence: 99%

Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies

Ritacco

Khetan

2018

Biotechnology Progress

182

138

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The culture of Chinese Hamster Ovary (CHO) cells for modern industrial applications, such as expression of recombinant proteins, requires media that support growth and production. Such media must support high viable cell densities while also stimulating the synthesis and extracellular transport of biologic products. Early media development efforts in this area yielded basic formulations to sustain growth, viability, and cellular function, albeit comprising animal sourced components, and complex constituents used in batch culture mode. Subsequent improvements included the development of serum‐free and chemically defined (CD) media, the identification of critical nutrients, growth factors, and potentially inhibitory or toxic cellular metabolites, and the use of fed‐batch and perfusion culture techniques to optimize nutrient delivery while minimizing accumulation of unwanted waste products. This review is comprised of sections covering milestones in the evolution of mammalian cell culture media, nutrient composition and formulation requirements, optimization strategies, consistency and scalability of powder and liquid media preparation for industrial applications, and key recent advances driving progress in CHO cell culture medium design and development. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1407–1426, 2018

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Section: Growth Factorsmentioning

confidence: 99%

Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies

Ritacco

Khetan

2018

Biotechnology Progress

182

138

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“…The addition of iron to CCM was reported to increase site‐occupancy of the glycoprotein tissue plasminogen activator 21 . Moreover, a constant glycosylation pattern was detected for interferon gamma (IFN‐γ) upon iron citrate addition to the medium compared to the non‐supplemented version, in which increased levels of non‐glycosylated IFN‐γ were observed 22 . Furthermore, the addition of iron to CCM was shown to significantly increase galactosylation of the recombinant glycoprotein, which has been disclosed in US Patent No.…”

Section: Introductionmentioning

confidence: 99%

Impact of iron raw materials and their impurities onCHOmetabolism and recombinant protein product quality

Weiss

Merkel

Zimmer

2021

Biotechnol Progress

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“…Among our selected hubs we identified 221 TFs belonging to, among others, the NAC, MYB, C2H2, bZIP, bHLH, ERF, and TALE TF families (Supplementary Table S4 ). These hub TFs also include homologs of TFs with known functions during SCW formation, such as SND2 ( SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN 2 ; Hussey et al, 2011 ), MYB46 (McCarthy et al, 2009 ; Ko et al, 2014 ) and HDG11 ( HOMEODOMAIN GLABROUS 11 ; Xu et al, 2014 ). Furthermore, we identified 21 Populus homologs of A. thaliana TFs that have been shown to bind to promoters of cellulose, xylan and lignin biosynthesis genes (Supplementary Table S4 ; Taylor-Teeples et al, 2015 ).…”

Section: Resultsmentioning

confidence: 99%

Ethylene-Related Gene Expression Networks in Wood Formation

et al. 2018

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Thickening of tree stems is the result of secondary growth, accomplished by the meristematic activity of the vascular cambium. Secondary growth of the stem entails developmental cascades resulting in the formation of secondary phloem outwards and secondary xylem (i.e., wood) inwards of the stem. Signaling and transcriptional reprogramming by the phytohormone ethylene modifies cambial growth and cell differentiation, but the molecular link between ethylene and secondary growth remains unknown. We addressed this shortcoming by analyzing expression profiles and co-expression networks of ethylene pathway genes using the AspWood transcriptome database which covers all stages of secondary growth in aspen (Populus tremula) stems. ACC synthase expression suggests that the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is synthesized during xylem expansion and xylem cell maturation. Ethylene-mediated transcriptional reprogramming occurs during all stages of secondary growth, as deduced from AspWood expression profiles of ethylene-responsive genes. A network centrality analysis of the AspWood dataset identified EIN3D and 11 ERFs as hubs. No overlap was found between the co-expressed genes of the EIN3 and ERF hubs, suggesting target diversification and hence independent roles for these transcription factor families during normal wood formation. The EIN3D hub was part of a large co-expression gene module, which contained 16 transcription factors, among them several new candidates that have not been earlier connected to wood formation and a VND-INTERACTING 2 (VNI2) homolog. We experimentally demonstrated Populus EIN3D function in ethylene signaling in Arabidopsis thaliana. The ERF hubs ERF118 and ERF119 were connected on the basis of their expression pattern and gene co-expression module composition to xylem cell expansion and secondary cell wall formation, respectively. We hereby establish data resources for ethylene-responsive genes and potential targets for EIN3D and ERF transcription factors in Populus stem tissues, which can help to understand the range of ethylene targeted biological processes during secondary growth.

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Effect of iron sources on the glycosylation macroheterogeneity of human recombinant IFN-γ produced by CHO cells during batch processes

Cited by 8 publications

References 3 publications

Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies

Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies

Impact of iron raw materials and their impurities onCHOmetabolism and recombinant protein product quality

Ethylene-Related Gene Expression Networks in Wood Formation

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