Effect of iron oxide and gold nanoparticles on bacterial growth leading towards biological application

Chatterjee, Saptarshi; Bandyopadhyay, A. K.; Sarkar, Keka

doi:10.1186/1477-3155-9-34

Cited by 163 publications

(92 citation statements)

References 18 publications

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“…Chatterjee et al 17 showed that nanoparticles of iron oxide have an inhibitory effect on Escherichia coli in a concentration dependent manner. The magnetic property of the iron oxide nanoparticles make them more promising in the targeted therapy of infectious disease.…”

Section: Introductionmentioning

confidence: 99%

Reduced <em>Staphylococcus aureus</em> biofilm formation in the presence of chitosan-coated iron oxide nanoparticles

Shi

Jia

Guo³

et al. 2016

IJN

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Abstract:Staphylococcus aureus can adhere to most foreign materials and form biofilm on the surface of medical devices. Biofilm infections are difficult to resolve. The goal of this in vitro study was to explore the use of chitosan-coated nanoparticles to prevent biofilm formation. For this purpose, S. aureus was seeded in 96-well plates to incubate with chitosan-coated iron oxide nanoparticles in order to study the efficiency of biofilm formation inhibition. The biofilm bacteria count was determined using the spread plate method; biomass formation was measured using the crystal violet staining method. Confocal laser scanning microscopy and scanning electron microscopy were used to study the biofilm formation. The results showed decreased viable bacteria numbers and biomass formation when incubated with chitosan-coated iron oxide nanoparticles at all test concentrations. Confocal laser scanning microscopy showed increased dead bacteria and thinner biofilm when incubated with nanoparticles at a concentration of 500 µg/mL. Scanning electron microscopy revealed that chitosan-coated iron oxide nanoparticles inhibited biofilm formation in polystyrene plates. Future studies should be performed to study these nanoparticles for anti-infective use.

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Section: Introductionmentioning

confidence: 99%

Reduced <em>Staphylococcus aureus</em> biofilm formation in the presence of chitosan-coated iron oxide nanoparticles

Shi

Jia

Guo³

et al. 2016

IJN

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“…1,2 There is an interest in the synthesis of nanomaterials because they exhibit AgNPs can be synthesized by chemical, 18 physical, 19,20 or biological routes involving plants 8,17,21,22 or microorganisms. [23][24][25][26][27][28] Biological synthesis is preferred nowadays because these methods are safe, cheap, eco-friendly, and do not involve any toxic substrate or by-product.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics

Singh¹,

Wagh²,

Wadhwani³

et al. 2013

IJN

116

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Background The development of nontoxic methods of synthesizing nanoparticles is a major step in nanotechnology to allow their application in nanomedicine. The present study aims to biosynthesize silver nanoparticles (AgNPs) using a cell-free extract of Acinetobacter spp. and evaluate their antibacterial activity. Methods Eighteen strains of Acinetobacter were screened for AgNP synthesis. AgNPs were characterized using various techniques. Reaction parameters were optimized, and their effect on the morphology of AgNPs was studied. The synergistic potential of AgNPs on 14 antibiotics against seven pathogens was determined by disc-diffusion, broth-microdilution, and minimum bactericidal concentration assays. The efficacy of AgNPs was evaluated as per the minimum inhibitory concentration (MIC) breakpoints of the Clinical and Laboratory Standards Institute (CLSI) guidelines. Results Only A. calcoaceticus LRVP54 produced AgNPs within 24 hours. Monodisperse spherical nanoparticles of 8–12 nm were obtained with 0.7 mM silver nitrate at 70°C. During optimization, a blue-shift in ultraviolet-visible spectra was seen. X-ray diffraction data and lattice fringes ( d =0.23 nm) observed under high-resolution transmission electron microscope confirmed the crystallinity of AgNPs. These AgNPs were found to be more effective against Gram-negative compared with Gram-positive microorganisms. Overall, AgNPs showed the highest synergy with vancomycin in the disc-diffusion assay. For Enterobacter aerogenes , a 3.8-fold increase in inhibition zone area was observed after the addition of AgNPs with vancomycin. Reduction in MIC and minimum bactericidal concentration was observed on exposure of AgNPs with antibiotics. Interestingly, multidrug-resistant A. baumannii was highly sensitized in the presence of AgNPs and became susceptible to antibiotics except cephalosporins. Similarly, the vancomycin-resistant strain of Streptococcus mutans was also found to be susceptible to antibiotic treatment when AgNPs were added. These biogenic AgNPs showed significant synergistic activity on the β-lactam class of antibiotics. Conclusion This is the first report of synthesis of AgNPs using A. calcoaceticus LRVP54 and their significant synergistic activity with antibiotics resulting in increased susceptibility of multidrug-resistant bacteria evaluated as per MIC breakpoints of the CLSI standard.

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“…A main advantage of the use of silver nitrate and silver compounds in general, for antibacterial infections, is the fact that Ag + is relatively nontoxic to human and animal cells, and in addition is very effective against fungi and viruses. It has been reported (12) that silver nitrate kills bacteria at higher concentrations than AgNPs, whereas SEM and X-ray diffraction data have shown that at lower silver nitrate concentrations bacteria may synthesize nanoparticles (13,14). Even though the reaction of Ag + ions with bacteria has been studied in depth for long periods of time, the inactivation mechanism is not entirely known and the reaction of nanoparticles with these pathogens is even less well understood (15).…”

Section: Significancementioning

confidence: 99%

Synergistic reaction of silver nitrate, silver nanoparticles, and methylene blue against bacteria

Chen

Cesario

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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In this paper we describe the antibacterial effect of methylene blue, MB, and silver nitrate reacting alone and in combination against five bacterial strains including Serratia marcescens and Escherichia coli bacteria. The data presented suggest that when the two components are combined and react together against bacteria, the effects can be up to three orders of magnitude greater than that of the sum of the two components reacting alone against bacteria. Analysis of the experimental data provides proof that a synergistic mechanism is operative within a dose range when the two components react together, and additive when reacting alone against bacteria.bacteria | synergistic effect | methylene blue | silver ions B acterial infections cause countless deaths in humans and animals. The increasing resistance and immunity of many bacteria to antibiotics demands that an effective means for the inactivation of bacteria becomes available that can overcome or negate the mechanism that bacteria use to inactivate the reactive agents before they render their lethal action on those organisms. The two antibacterial agents that we used in this study were methylene blue (MB) and silver nitrate. Each of these alone is known to be an effective antibacterial agent. The data presented in this study show that they make a synergistic agent pair against the five bacteria studied. MB is used as a bacterial inactivator for skin treatment, dental therapy, and other areas in need of bacterial disinfection (1-3). MB is a cation, whereas in its reduced leuco form MB has a pK a of 5.8 and low ionization at neutral physiological pH. This enables bacteria to reduce and inactivate MB to the leuco form. In fact, the discoloration of MB has been an indication of the presence of bacteria. In its cationic form, MB irradiated with 630-680-nm light is used for cancer therapy and as an effective bactericidal and activating agent. MB is an effective antibacterial agent owing to its ability to generate a high concentration of singlet oxygen, 1 O 2 ( 1 Δ g ), and other reactive oxygen species, such as OH • radicals (ROS), when irradiated with red light, which in contrast to UV light does not harm humans or animals cells. Therefore, the MB bacterial inactivation process is very safe and effective owing to the high reactivity of ROS with bacterial outer-cell membrane. It is found that the photogenerated singlet oxygen reacts against tryptophan and other outer-membrane molecules, resulting in damage to the bacterial cell and inhibiting protein production. ROS may also create a hole in the bacterial cell wall, which allows MB radicals to penetrate through the bacterial membrane and attack DNA, forming dimers which prevent it from replicating and consequently induce bacterial inactivation. The photoreaction that generates singlet oxygen and OH • radicals proceeds as follows: i) MB in water solution is excited to the first singlet state MB*:MB + hν (660 nm) →MB*; ii) MB* decays to the triplet state 3 MB* with a lifetime of ∼10 −9 s: MB* → 3 MB*; iii) The tripl...

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Effect of iron oxide and gold nanoparticles on bacterial growth leading towards biological application

Cited by 163 publications

References 18 publications

Reduced <em>Staphylococcus aureus</em> biofilm formation in the presence of chitosan-coated iron oxide nanoparticles

Reduced <em>Staphylococcus aureus</em> biofilm formation in the presence of chitosan-coated iron oxide nanoparticles

Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics

Synergistic reaction of silver nitrate, silver nanoparticles, and methylene blue against bacteria

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