Effect of iron overload on renal functions and oxidative stress in beta thalassemia patients

Rasool, Mahmood; Malik, Arif; Jabbar, Uzma; Begum, Irshad; Qazi, Mahmood Husain; Asif, Muhammad; Naseer, Muhammad Imran; Ansari, Shakeel Ahmed; Jarullah, Jummanah; Haque, Absarul; Jamal, Mohammad Sarwar

doi:10.15537/smj.2016.11.16242

Cited by 16 publications

(11 citation statements)

References 10 publications

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“…induced oxidative stress in the mitochondria, and long-term excess dietary iron damaged liver mitochondrial function (Volani et al, 2017;Atarashi et al, 2018). Serum creatinine and uric acid were increased in iron-overload thalassemia patients, indicating iron overload corrected with kidney dysfunction (Rasool et al, 2016). However, no significant changes were observed in the serum parameter relation with liver and kidney functions in this study.…”

Section: Discussioncontrasting

confidence: 55%

Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Chen

Wang

et al. 2020

Front. Cell Dev. Biol.

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Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron supplementation on growth performance, the distribution pattern of iron in the gut lumen and the host, intestinal microbiota, and intestine transcript profile of piglets. Sixty healthy weaning piglets were randomly assigned to six groups: fed on diets supplemented with ferrous sulfate monohydrate at the dose of 50 ppm (Fe50 group), 100 ppm (Fe100 group), 200 ppm (Fe200 group), 500 ppm (Fe500 group), and 800 ppm (Fe800), separately, for 3 weeks. The results indicated that increasing iron had no significant effects on growth performance, but increased diarrheal risk and iron deposition in intestinal digesta, tissues of intestine and liver, and serum. High iron also reduced serum iron-binding capacity, apolipoprotein, and immunoglobin A. The RNA-sequencing analysis revealed that iron changed colonic transcript profile, such as interferon gamma-signal transducer and activator of transcription two-based anti-infection gene network. Increasing iron also shifted colonic and cecal microbiota, such as reducing alpha diversity and the relative abundance of Clostridiales and Lactobacillus reuteri and increasing the relative abundance of Lactobacillus and Lactobacillus amylovorus. Collectively, this study demonstrated that high dietary iron increased diarrheal incidence, changed intestinal immune response-associated gene expression, and shifted gut microbiota. The results would enhance our knowledge of iron effects on the gut and microbiome in piglets and further contribute to understanding these aspects in humans.

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Section: Discussioncontrasting

confidence: 55%

Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Chen

Wang

et al. 2020

Front. Cell Dev. Biol.

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“…In thalassemia patients, the iron overload due to transfusions negatively affects the renal function and ferritin, blood urea nitrogen, creatinine, uric acid, albumin were significantly higher in patients compared to the control. Ferritin is a protein located inside the cell that plays a decisive role in the iron storage, being useful in the evaluation of deficiency or overload of iron, but it is an insufficient and unreliable test if it is not associated with other blood tests [23].…”

Section: Resultsmentioning

confidence: 99%

Influence of Iron Administration on Kidney Serum Parameters

Dronca¹,

Peţ²,

Ştef³

et al. 2020

Rev.Chim.

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Some minerals are essential nutrients for organisms being necessary in different quantities, but any homeostasis disturbance (deficiency or excess) can lead to various diseases. Iron in the body plays a critical role being directly involved in the respiration process - more precisely O2 and CO2 transportation, and also in beneficial oxidation-reduction processes. But sometimes the iron can also lead to the formation of substances with toxicogenic potential, more precisely to free radicals formed as a result of oxidative stress. In the liver, there is a specialized protein - hepcidin - which is the key regulator of circulating iron in the organism. In several cases of overdose, this protein blocks the iron transportation, binds it to the export channels of ferroportin and finally sends the excess iron to the kidney for urinal excretion. Our experiment tried to evaluate if in case of iron intraperitoneal administration in excess to rabbits, for a short period of time, it influences the serum creatinine, uric acid, and blood urea nitrogen if the rabbits were fed with a special organic diet. The results of the experiment presented a lower concentration of creatinine (with 24.46%), uric acid (with more than 41.48%), and blood urea nitrogen - BUN (6.45%) for experimental rabbits compared to the control group, proving that a well-documented diet influences the accumulation and excretion of excess iron. Keywords: iron, creatinine, uric acid, blood urea nitrogen

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“…High serum malondialdehyde and serum ferritin levels in patients with thalassemia were significant risk factors for renal cell damage in such patients. 11 There is a recent report of the role of metal, such as manganese, in the pathogenesis of neurodegenerative diseases. 12 The redox homeostasis is important in controlling oxidative stress related cell death, such as thioredoxin, an antioxidant system, that controls cell fate.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Transfusion-Dependent Thalassemia Patient’s Serum on Peripheral Blood Mononuclear Cell Viability

et al. 2019

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Iron overload is a major complication in transfusion-dependent thalassemia (TDT) patients. Chronic oxidative stress from iron overload may lead to cellular damage and viability. This is a cross-sectional study. Transfusion-dependent thalassemia patients aged ⩾18 years old were enrolled. Transfusion-dependent thalassemia patient’s serum and normal volunteer’s serum were separately incubated with healthy peripheral blood mononuclear cells (PBMCs). The cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay at 24, 48, and 72 hours. Sixty-nine TDT patients and 22 healthy controls were enrolled. The mean of PBMCs viability after incubation with serum from TDT patients was lower than that with the controls (88.65% vs 103.56% at 24 hours, 78.77% vs 112.04%% at 48 hours, and 71.18% vs 132.16%% at 72 hours, respectively). High serum ferritin level (correlation −0.29, P < .05) and white blood cell (WBC) count negatively affected cell viability (correlation −2.86, P = .05). From multivariate analysis, serum ferritin level is the only significant risk factor that is independently associated with cell viability (correlation −11.42, P < .001). Our findings showed that TDT patient’s serum causes decreased cell viability. Serum ferritin level was a significant independent factor influencing cell viability.

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Effect of iron overload on renal functions and oxidative stress in beta thalassemia patients

Cited by 16 publications

References 10 publications

Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Influence of Iron Administration on Kidney Serum Parameters

The Effect of Transfusion-Dependent Thalassemia Patient’s Serum on Peripheral Blood Mononuclear Cell Viability

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