Effect of iron on inhibition of acid demineralisation of bovine dental enamel in vitro

Buzalaf, Marília Afonso Rabelo; Italiani, Flávia de Moraes; Kato, Melissa Thiemi; Martinhon, Cleide Cristina Rodrigues; Magalhães, Ana Carolina

doi:10.1016/j.archoralbio.2006.04.007

Cited by 32 publications

(42 citation statements)

References 21 publications

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“…Although many studies have demonstrated that Fe 2+ has a protective effect against demineralization by acid challenges [Pecharki et al, 2005;Buzalaf et al, 2006;Martinhon et al, 2006;Kato et al, 2007a, b;Sales-Peres et al, 2007;Kato et al, 2009;Alves et al, 2011], it was not able to promote remineralization of artificial caries lesions in vitro [Alves et al, 2011]. To elucidate the mechanism of Fe 2+ action, this study aimed to verify the influence of Fe 2+ on the structure of HA using synthetic HA powder and an in vitro model that simulates dissolution and precipitation.…”

Section: Discussionmentioning

confidence: 99%

“…Alone, or in combination with other ions such as fluoride and copper, iron has a beneficial impact on the decrease of the cariogenic potential of sugar [Oppermann and Rölla, 1980;Rosalen et al, 1996;Miguel et al, 1997a, b]. Many in vitro and in situ studies showed that Fe 2+ reduces enamel demineralization in highly cariogenic and erosive challenges [Pecharki et al, 2005;Buzalaf et al, 2006;Martinhon et al, 2006;Kato et al, 2007a, b;Sales-Peres et al, 2007;Kato et al, 2009;Alves et al, 2011], which proves the effectiveness of Fe an acid-resistant layer (ferric phosphate) [Torell, 1988], thereby decreasing enamel mineral loss. However, Alves et al [2011] demonstrated that ferrous (Fe 2+ ) sulfate was not able to remineralize artificial caries lesions.…”

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confidence: 99%

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Effect of Iron II on Hydroxyapatite Dissolution and Precipitation in vitro

Delbem¹,

Alves²,

Sassaki

et al. 2012

Caries Res

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The aim of this study was to evaluate the effect of iron II on the dissolution and precipitation of synthetic hydroxyapatite (HA). HA powder was suspended in solutions of iron (0.84 µg/ml, Fe0.84; 18.0 µg/ml, Fe18; 70.0 µg/ml, Fe70), fluoride (1,100 µg/ml, F1,100), and deionized water and submitted to pH cycling. After pH cycling, the samples were analyzed by infrared spectroscopy and X-ray diffraction. The concentrations of fluoride, calcium, phosphorus, and iron were also analyzed. The data were submitted to ANOVA, and analyzed by Tukey’s test (p < 0.05). The infrared spectrum showed a reduction in all bands corresponding to phosphates and hydroxyls and an increase in the carbonate band in the groups with iron. The intensity of the phosphate bands increased and that of the hydroxyl bands decreased in the group F1,100. It was observed that there was a higher concentration of Ca in the group F1,100, with no significant difference between the groups Fe18 and Fe70 (p > 0.05). There was an increase in Fe concentration in the HA directly related to the Fe concentration of the treatment solutions. Results show that the presence of Fe causes the precipitation of apatite with high solubility.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of Iron II on Hydroxyapatite Dissolution and Precipitation in vitro

Delbem¹,

Alves²,

Sassaki

et al. 2012

Caries Res

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“…One paper clearly attributed a reduction of the mineral solubility of hydroxyapatite to the zinc ion [Brudevold et al, 1963], whereas another study found no effect [McLundie et al, 1968]. Other ions like ferrous [Buzalaf et al, 2006] and aluminium ions [Putt and Kleber, 1985] are also said to diminish the solubility of hydroxyapatite.…”

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confidence: 99%

Investigation of the Effect of Various Fluoride Compounds and Preparations Thereof on Erosive Tissue Loss in Enamel in vitro

et al. 2009

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Metal fluoride preparations are promising in view of protection against dental erosion. The aim of this study was to screen the effect fluoride preparations containing various polyvalent metal cations had on erosion progression under increasingly severe erosive conditions in human enamel in vitro. Specimens were subjected to a cyclic de- and remineralisation procedure. Demineralisation (experiment E1 and E2a: 6 × 2 min/day, experiment E2b: 6 × 5 min/day) was performed with 0.05 M citric acid (pH 2.3). Fluoridation directly followed demineralisation and was reduced stepwise (E1: 6 × 2 min/day, E2a and E2b: 2 × 2 min/day). The test solutions were: ZnF₂(E1), AmF/NaF/CuSO₄ (E1), TiF₄/AmF/Zn lactate (E1), TiF₄ (E1, E2a), TiF₄/AmF (E2a), TiF₄/AmF/NaF (E2a), TiF₄/AmF/ZnF₂ (E1, E2a, E2b), AmF/NaF/SnCl₂ (2,800 mg/l Sn²⁺; E1, E2a, E2b), AmF/NaF/SnCl₂-1 (700 mg/l Sn²⁺; E2a, E2b), all 1,500 ppm F^–, pH 4.5, and AmF/SnF₂ (positive control, pH 4.2, 250 ppm F^–; E1, E2a, E2b). In the negative control group, specimens were not fluoridated. Tissue loss was determined by profilometry after the last experimental day. Under mild conditions (E1), tin- and titanium-containing solutions were of marked efficacy and could nearly completely inhibit erosive tissue loss (reduction between 94.2 and 97.5%). With increasingly severe conditions (E2a, E2b), titanium lost its efficacy. Only the highly concentrated tin preparation (2,800 mg/l Sn²⁺) was able to reduce erosive tissue loss by 93.1%, even under severe conditions (E2b), with less frequent application of the experimental preparations. It should be considered for approaches for a symptomatic therapy of dental erosion.

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“…Thereby, the literature has shown that after the acid challenge and in the presence of FS, the ferric and phosphates ions dissolved in the surface of the enamel are precipitated and an acid-resistant surface is established in the enamel. It is possible that in anemic children treated with FS and presenting caries, these lesions may become inactive [7][8][9]13,24 .…”

Section: Authors Contributionmentioning

confidence: 99%

“…Iron supplements are well tolerated by most infants, have no measurable effect on body growth in children, and early iron supplementation, even in breastfed infants, is feasible and transiently increases iron status 4 . FS has been analyzed in several studies in dentistry [5][6][7][8][9][10][11][12][13] using artificial dental models (mainly bovine teeth and human permanent teeth). The most frequently reported properties include reduction in the degree of enamel dissolution 5,8,13 , reduction of dental erosion 7,12 , alterations in biofilm composition (in situ) 9,10,14 , and oxidative modifications in glucosyltransferase promotion 11 .…”

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confidence: 99%

Evaluation of the Effect of Ferrous Sulfate on Enamel Demineralization of Human Deciduous Teeth: An in Vitro Study

Gauw¹,

Costa²,

Silva³

et al. 2017

RevBahianaOdonto

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| Objective:This study aimed to evaluate the effect of ferrous sulfate (FS) on demineralized and nondemineralized human deciduous teeth. Additionally, it was evaluated the penetration extent of FS and its remineralizing effect on the enamel of deciduous teeth using Polarized Light Microscopy (PLM). Method: The sample comprised 44 human deciduous teeth. The 44 crowns were divided randomly into four groups: group A (FS after demineralization), group B (FS without demineralization), group C (only demineralization), and group D (control group). FS at 0.45 mol/L -1 was used daily (15 days) and demineralization was done by pH cycling (7 days). Then, three longitudinal slices of the crowns were photographed using PLM. The degree of penetration of the lesion or stain was measured in micrometers, as well as the distance between the external enamel surface and the core of lesion. Results: Group A showed a dark stain on the outer surface of enamel larger than the group B. It is suggested, a remineralizing effect when comparing groups, A and C. The mean depth and standard deviation for groups A, B, and C were 4.27 µm (±1.49), 3.72 µm (±1.68) and 5.00 µm (±1.84), respectively. No dark stains were observed in group D. Conclusion: FS stained the demineralized and non-demineralized human deciduous teeth. However, dark stains in the non-demineralized teeth were smaller or absent, than in the demineralized teeth. Therefore, FS may have a protective effect against demineralization.Keywords: Ferrous sulfate, iron deficiency, dental caries, deciduous teeth. RESUMO | Objetivo:Este estudo objetivou avaliar o efeito do sulfato ferroso (SF) em dentes decíduos humanos desmineralizados e não desmineralizados. Além disso, avaliou-se a extensão da penetração do SF e seu efeito remineralizante no esmalte de dentes decíduos usando microscopia de luz polarizada (MLP). Método: A amostra foi formada por 44 dentes decíduos humanos. As 44 coroas foram divididas aleatoriamente em quatro grupos: A (SF após desmineralização), B (SF sem desmineralização), C (apenas desmineralização) e D (grupo controle). O SF a 0,45 mol/L -1 foi utilizado diariamente (15 dias) e a desmineralização foi feita por ciclagem de pH (7 dias). Em seguida, três fatias longitudinais das coroas foram fotografadas usando MLP. O grau de penetração da lesão ou mancha foi medido em micrômetros, bem como a distância entre a superfície externa do esmalte e o núcleo da lesão. Resultados: O grupo A mostrou uma mancha escurecida na superfície externa do esmalte maior do que o grupo B. Sugere-se, um efeito remineralizante na comparação dos grupos, A e C. A profundidade média e o desvio padrão para os grupos A, B e C foram de 4,27 µm (± 1,49), 3,72 µm (± 1,68) e 5,00 µm (± 1,84), respectivamente. Não foram observadas manchas escurecidas no grupo D. Conclusão: O SF manchou os dentes decíduos humanos desmineralizados e não desmineralizados. No entanto, manchas escurecidas nos dentes não desmineralizados foram menores ou ausentes, do que nos dentes desmineralizados. Portanto, o SF po...

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Effect of iron on inhibition of acid demineralisation of bovine dental enamel in vitro

Cited by 32 publications

References 21 publications

Effect of Iron II on Hydroxyapatite Dissolution and Precipitation in vitro

Effect of Iron II on Hydroxyapatite Dissolution and Precipitation in vitro

Investigation of the Effect of Various Fluoride Compounds and Preparations Thereof on Erosive Tissue Loss in Enamel in vitro

Evaluation of the Effect of Ferrous Sulfate on Enamel Demineralization of Human Deciduous Teeth: An in Vitro Study

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