Effect of ionizing radiation on the proliferation of human embryonic stem cells

Abstract

We studied the effect of ionizing radiation (IR) on continuous growth of seven hESC lines. Cells were exposed to 0, 0.2, or 1 Gy of X-rays, and the growth rates of cell populations were assessed by measuring areas of the same individual colonies versus time. The population doubling times (DT) of sham-irradiated cells varied from 18.9 to 28.7 hours for different cell lines. All cell lines showed similar reaction to IR, i.e. cell populations dropped within 24–48 hours post IR; after that they recovered and grew with the same rate as the sham-irradiated cells. The relative cell survival (RCS), i.e. the ratio of normalized cell population in the irradiated samples to that of the sham-irradiated ones varied from 0.6 to 0.8 after 0.2 Gy, and from 0.1 to 0.2 after 1 Gy IR for different cell lines. We found that the RCS values of hESC lines correlated directly with their DT, i.e. the faster cells grow the more radiosensitive they are. We also found that DT and RCS values of individual colonies varied significantly within all hESC lines. We believe that the method developed herein can be useful for assessing other cytotoxic insults on cultures of hESC.

The method to generate pulsatile circulatory fluid flow using microfluidics

“…We also found that the difference between hESC maintenance culture in standard tissue culture plates and the microfluidic device in a static condition was not significant ( p >0.05). The doubling time for hESC H9 in both culture systems matched that reported in the literature (doubling time: 24.2 h) [14] .

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Effect of Ionizing Radiation from Computed Tomography on Differentiation of Human Embryonic Stem Cells into Neural Precursors

“…Viability of hESCs after exposure to radiation was similar to that reported previously [29]. Herein, the reaction of hESCs to CT irradiation was assessed by the expression of pluripotency marker OCT4 and the DNA repair foci marker 53BP1 [19].…”

Xenogeneic-Free System for Biomanufacturing of Cardiomyocyte Progeny From Human Pluripotent Stem Cells

“…Stem cell proliferation rate depends on multiple factors including genetic background, culture media and conditions, and passage number (Park et al, 2008). For instance, reported doubling times for H9 hESCs vary from around 24 h (Panyutin et al, 2017) to 133 h for a microcarrier culture study (Badenes et al, 2016). Compared to the other hPSC lines, H9 hESCs here exhibiting a greater expansion rate and a decline from a peak concentration of ∼27 × 10 5 cells/ml a day after commencement of the differentiation to ∼20 × 10 5 cells/ml at the end.…”