The method to generate pulsatile circulatory fluid flow using microfluidics

Li, Jingjing; Lao, Osmond; Nordon, Robert E.

doi:10.1016/j.mex.2021.101269

Cited by 4 publications

(4 citation statements)

References 13 publications

(19 reference statements)

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“…Direct visualization of EHT in human embryonic aorta is not possible, so current knowledge relies on single-cell transcriptomics of micro-dissected human embryonic aorta and in vitro analysis of hESC-derived hematopoiesis (Calvanese et al, 2022;Crosse et al, 2020;Ng et al, 2016;Zeng et al, 2019). Therefore, we have developed a microfluidic pulse generator (MPG) (Figures 1A and 1B) to simulate and observe the influence of embryonic circulation on hESC-derived blood formation (Chen et al, 2013;Li et al, 2021). The MPG drives flow around a microfluidic circuit with a 2-3 mL circulatory volume (Figures 1A-1C, see STAR Methods: Fabrication of the microfluidic pulsatile flow generator; Figure S1; Videos S1 and S2).…”

Section: Resultsmentioning

confidence: 99%

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Mimicry of embryonic circulation enhances the hoxa hemogenic niche and human blood development

Lao

Bruveris

et al. 2022

Cell Reports

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Section: Resultsmentioning

confidence: 99%

“…The detailed description of material and method for making MPG has been described previously (Li et al, 2021).…”

Section: Materials Availabilitymentioning

confidence: 99%

Mimicry of embryonic circulation enhances the hoxa hemogenic niche and human blood development

Lao

Bruveris

et al. 2022

Cell Reports

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“…EC under physiological WSS condition could change its alignment, elongation, and gene expression (34,39). The different settings of the WSS value, and flow conditions such as the pulsatile flow may affect the EC activities mentioned in the refs (40)(41)(42).…”

Section: Limitationmentioning

confidence: 99%

Migration of endothelial cells on the surface of anodized Ni-Ti stent strut

Wang

Ohtsu

Tate

et al. 2023

Front. Med. Technol.

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BackgroundStent is widely regarded as the main treatment for curing cardiovascular diseases such as stenosis. Previous research has revealed that the damage of endothelial cells (EC), i.e., the components of endothelium, during stent implantation, could lead to severe complications, such as restenosis. To prevent restenosis, enhancements have been made to surface biocompatibility to accelerate the stent endothelialization process. Anodization on the Ni-Ti is a simple and efficient surface modification method to improve the biocompatibility of the Ni-Ti stent surfaces by enhancing the surface hydrophilicity, leading to an increase in the EC activities. The EC activity is known to be affected by the blood flow. Flow change by stent structure may result in EC dysfunctions, thereby leading to restenosis. It is thus essential to investigate the EC activities resulting from the anodization on the Ni-Ti surface under flow conditions.ObjectiveTo study the influence of the endothelialization process on the Ni-Ti stent surface through anodization. The EC attachment and morphology on the anodized stent strut were observed under both with and without the flow conditions.MethodA parallel plate flow chamber was designed to generate a constant wall shear stress (WSS) to study the flow effect on the EC behavior. The hydrophilicity of the Ni-Ti stent strut surface was enhanced by a TiO2 layer fabricated via anodization. The EC distribution on the surface of the anodized nitinol stent strut was observed after 24 h of static (without flow) and flow exposure (with flow) experiment.ResultsUnder the static condition, the EC density on the surface of the anodized Ni-Ti stent strut was higher compared with the control. Under the flow condition, the enhancement of the EC density on the surface of the stent strut with anodization was reduced. The EC demonstrates a long and thin spindle-shaped morphology under the flow condition.ConclusionUnlike the static condition, the EC is demonstrating a long and thin morphology in response to the flow under the flow condition. By improving the surface hydrophilicity, the anodization could enhance the EC migration onto the strut surface, and subsequently, accelerate the Ni-Ti stent endothelialization process. The improvement of the surface hydrophilicity is lower under the flow conditions when compared with the static conditions.

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“…Nandakumar et al 38 analysed the pulsating flow of a blood through a 2-D stenosed channel and the authors also investigated the effect of Womersley number. A nanomaterials system with pressure-driven microvalves and ventricles which generates pulsatile cardiovascular flow is studied by Li et al 39 .…”

Section: Introductionmentioning

confidence: 99%

Impacts of Joule heating and viscous dissipation on MHD pulsatile flow of third grade nanofluid in a channel

Govindarajulu

Reddy

2022

Proceedings of the Institution of Mechanical Engineers, Part E:

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The current exploration deals with the third grade hydromagnetic pulsating flow of blood-gold nanofluid in a channel with the presence of Ohmic heating, viscous dissipation and radiative heat. In the present analysis, blood (base fluid) is considered as third-grade fluid and gold (Au) as nanoparticle. This investigation is useful in the fields of food processing system, pressure surges (pulsatile flow application), biomedical engineering, nano drug delivery, radiotherapy, and cancer therapeutic (nanofluid application). Perturbation method is employed to transform the set of governing partial differential equations (PDEs) into the ordinary differential equations (ODEs) and then solved by employing the fourth order Runge-Kutta method with the aid of the shooting technique. The impacts of emerging dimensionless parameters of velocity, temperature, and heat transfer rate of blood-Au nanofluid are analysed via pictorial outcomes in detail. The obtained results depict that the improvement in viscous dissipation and heat source enhanced the temperature of third grade nanofluid. The velocity and temperature of the nanofluid are declining functions with the enhancement of frequency parameter, material parameter, and non-Newtonian parameter respectively. Intensifying the volume fraction of nanoparticle dwindles the velocity and temperature of nanofluid. Enhancing volume fraction and viscous dissipation accelerates the heat transfer rate of nanofluid. The velocity, temperature, and heat transfer rates are decreased by an escalation of the Hartmann number. Further, enhancing the radiation parameter reduces the heat transfer rate and temperature of nanofluid.

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The method to generate pulsatile circulatory fluid flow using microfluidics

Cited by 4 publications

References 13 publications

Mimicry of embryonic circulation enhances the hoxa hemogenic niche and human blood development

Mimicry of embryonic circulation enhances the hoxa hemogenic niche and human blood development

Migration of endothelial cells on the surface of anodized Ni-Ti stent strut

Impacts of Joule heating and viscous dissipation on MHD pulsatile flow of third grade nanofluid in a channel

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