Effect of intravenous pancreozymin on amino acid incorporation in vitro by pancreatic tissue

Effect of caerulein on protein synthesis and secretion in the guinea‐pig pancreas

“…Since it is known that most of the newly synthesized protein is accounted for by exportable (pro)enzymes (Jamieson & Palade, 1967b;Poort & Kramer, 1969), the constancy in the rate of the total protein synthesis that we observed under these conditions indicates that the synthesis of exportable protein does not undergo significant fluctuations. A different situation has been found in the pigeon, where stimulation in vivo (and not in vitro) brings about an increase of protein synthesis (Webster & Tyor, 1966;Peakall, 1967;Webster, 1968), and also in frogs, where feeding after prolonged starvation is equally effective (Poort & Geuze, 1969 (Bertaccini et al, 1969;Erspamer, 1970). Given during starvation 2C when the rate of protein synthesis is greatly reduced and secretion of pancreozymin is also hampered, caerulein is capable of partially restoring the proteosynthetic activity of pancreatic cells, while in fed animals it is completely ineffective.…”

“…Several conflicting results concerning the effect of stimulation of pancreatic secretion on the rate of protein synthesis have been published in the past (Daly & Mirsky, 1953;Allfrey et al, 1954;Ferreira et al, 1955 ;Farber & Sidransky, 1956;Hokin, 1956;Busch et al, 1959;Stocker, 1962;Webster & Tyor, 1966;Peakall, 1967;Webster, 1968;Kramer & Poort, 1968;Poort & Geuze, 1969;Poort & Kramer, 1969). As pointed out by Poort & Kramer (Kramer & Poort, 1968;Poort & Kramer, 1969) it is possible to reconcile most of the discrepancies by taking into account the fact that in a protein-secreting gland the amount of protein present Values given are averages ±S.E.…”

“…Numerous studies dealing with the effect of stimulation of enzyme secretion on the rate of protein synthesis in the pancreas have been reported (Daly & Mirsky, 1953;Allfrey, Daly & Mirsky, 1954;Ferreira Fernandez & Junqueira, 1955;Farber & Sidransky, 1956;Hokin, 1956;Busch, Allen & Anderson, 1959;Stocker, 1962;Webster & Tyor, 1966;Peakall, 1967;Webster, 1968;Kramer & Poort, 1968;Poort & Geuze, 1969;Poort & Kramer, 1969). Though several conflicting results have been obtained in the past, recent studies seem to indicate that a substantial increase of the rate of protein synthesis is brought about by either feeding or pharmacological stimulation of secretion only in birds and frogs (Webster & Tyor, 1966: Peakall, 1967Webster, 1968;Poort & Geuze, 1969); whereas in mammals protein synthesis proceeds at a constant rate, independently of secretion (Kramer & Poort, 1968;Poort & Kramer, 1969).…”

“…Though several conflicting results have been obtained in the past, recent studies seem to indicate that a substantial increase of the rate of protein synthesis is brought about by either feeding or pharmacological stimulation of secretion only in birds and frogs (Webster & Tyor, 1966: Peakall, 1967Webster, 1968;Poort & Geuze, 1969); whereas in mammals protein synthesis proceeds at a constant rate, independently of secretion (Kramer & Poort, 1968;Poort & Kramer, 1969).…”

Effect of Pancreozymin on Rat Pancreatic Enzyme Biosynthesis

“…Possibly because of the is mediated in part by the polypeptidic hormones, different systems and techniques used to stimupancreozymin and secretin . The specific physlate secretion or measure protein biosynthesis, iological role of these two hormones is now well opposite conclusions have been reached (Hokin, established. Pancreozymin is known to promote 1953 ; Siekevitz and Palade, 1958 ; Webster and the secretion of hydrolytic enzymes from the Tyor, 1966 ;Kramer and Poort, 1968) . Taking pancreas, while secretin acts on the secretion of advantage of the methods set up in our laboratory water and salts (Harper, 1967) .…”