Effect of Pancreozymin on Rat Pancreatic Enzyme Biosynthesis

Reggio, Hubert; Cailla-Deckmyn, H.; Marchis-Mouren, G.

doi:10.1083/jcb.50.2.333

Cited by 42 publications

(9 citation statements)

References 17 publications

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“…Such an increase, following stimulation by feeding has been demonstrated in frogs (Poort and Geuze 1969;Van Venrooij and Poort 1971) and snakes (Alcon and Bdolah 1975). In the rat, the rate of biosynthesis of pancreatic enzymes increased 8-10 fold, two hours after injection of a mixture of secretin and CCK (Reggio et al 1971). It is possible therefore that CCK may regulate pancreatic enzyme levels in teleosts, by control of both synthesis and the secretion.…”

Section: The Effect Of[eeding On Topsin and Chymotltpsin Synthesis mentioning

confidence: 90%

The effect of feeding on the secretion of pepsin, trypsin and chymotrypsin in the Atlantic salmon,Salmo salar L

Einarsson

Davies

Talbot

1996

Fish Physiol Biochem

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Pepsin stored in the stomach mucosa of the Atlantic salmon (Salmo salar L.) increases within two days of the onset of starvation. Trypsin and chymotrypsin in the pyloric caeca/pancreas behave similarly, indicating that when no food is present in the gut, digestive enzymes accumulate in the secretory tissues. As a corollary, trypsin and chymotrypsin activities in the gut contents fell during starvation, indicating that secretion is greatly reduced when food is not present. At the onset of feeding, pepsin is rapidly synthesised in the mucosal tissues and then secreted. Twenty four hours after feeding, the pepsin levels of the mucosa are still low, suggesting that synthesis may be a rapid response to the presence of food in the stomach. Secretion of trypsin and chymotrypsin appears to take place as soon as digesta enters the intestine, between 4 and 14h after feeding, and resynthesis of enzyme precursors appears to be complete again within a further 11h. It is suggested that both synthesis and release may be under the control of cholecystokinin.

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Section: The Effect Of[eeding On Topsin and Chymotltpsin Synthesis mentioning

confidence: 90%

The effect of feeding on the secretion of pepsin, trypsin and chymotrypsin in the Atlantic salmon,Salmo salar L

Einarsson

Davies

Talbot

1996

Fish Physiol Biochem

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“…With regards to the former, there is a stimulus to secretion since animals are suckling by 4 h of age, and there is almost certainly an increase in vagal activity at birth. It is still unclear from the literature, however, that stimulation of secretion results in increased synthesis of exportable protein [Reggio et at., 1971;Kramer and Poort, 1968;Morisset and Webster, 1972], The latter explanation, however, must be strongly con sidered. Marchis-Mouren et al [1963] and others have shown that the rate of synthesis of amylase is greater on a starch-rich diet than on a caseinrich diet, and that similar results are obtained if glucose is substituted for starch.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Pancreatic α-Amylase in the Neonatal Mouse

et al. 1973

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The decrease in specific activity of mouse pancreatic α-amylase occurring at parturition is associated with changes in the morphological fine structure of the acinar cells, as evidenced by a decrease in the number of zymogen granules per cell and an increase in the rough endoplasmic reticulum. Following parturition there is a rapid increase in the synthesis of both amylase and total protein, with a selective increase in amylase synthesis during the first 24 hours. Metabolic requirements for in vitro secretion of amylase by the neonatal mouse pancreas were also studied; findings support existing evidence that fatty acids are the main oxidizable substrate of the acinar cell.

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“…In marked contrast, little change in exocrine pancreatic function at the level of protein synthesis has been observed under similar conditions. Earlier studies showed conflicting results with modest increases in total protein synthesis in vivo after bolus injections of cholecystokinin (10)(11)(12)(13) and modest decreases in total synthesis during cholecystokinin stimulation in vitro (14). Since the exocrine pancreas synthesizes and secretes approximately 20 exportable proteins (15), anticoordinate changes in individual biosynthetic rates could explain the modest changes observed at the level of total protein synthesis.…”

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confidence: 99%

Hormonal stimulation in the exocrine pancreas results in coordinate and anticoordinate regulation of protein synthesis.

Schick¹,

Kern²,

Scheele³

1984

The Journal of Cell Biology

131

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24-h intravenous caerulein infusion studies in the rat were combined with in vitro amino acid incorporation studies followed by high- resolution separation of proteins by two-dimensional isoelectric focusing and SDS gel electrophoresis to study the extent to which persistent changes in the biosynthesis of exocrine pancreatic proteins are regulated by cholecystokinin-like peptides. Beginning in the third hour of optimal hormone infusion at 0.25 microgram kg-1 h-1, changes were observed in the synthetic rates of 12 proteins, which progressed over the course of the 24-h study. Based on coordinate response patterns, exocrine proteins could be classified into four distinct groups. Group I (trypsinogen forms 1 and 2) showed progressive increases in synthetic rates reaching a combined 4.3-fold increase over control levels. Group II (amylase forms 1 and 2) showed progressive decreases in synthesis to levels 7.1- and 14.3-fold lower than control levels, respectively. Group III proteins (ribonuclease, chymotrypsinogen forms 1 and 2, procarboxypeptidase forms A and B, and proelastase 1) showed moderate increases in synthesis, 1.4-2.8-fold, and group IV proteins (trypsinogen 3, lipase, proelastase 2, and unidentified proteins 1-4) did not show changes in synthesis with hormone stimulation. Regulation of protein synthesis in response to caerulein infusion was specific for individual isoenzymic forms in the case of both trypsinogen and proelastase. The ratio of biosynthetic rates of trypsinogen forms 1 + 2 to amylase forms 1 + 2 increased from a control value of 0.56 to 24.4 after 24 h of hormonal stimulation (43.5-fold increase). Biosynthetic rates for an unidentified protein (P23) with an Mr = 23,000 and isoelectric point of 6.2 increased 14.2- fold, and the ratio of synthesis of P23 to amylase 2 increased 200-fold during caerulein infusion. During hormone stimulation the anticoordinate response in the synthesis of pancreatic glycosidases (decreased synthesis) and serine protease zymogens (increased synthesis) explain previous observations that showed little change in rates of total protein synthesis under similar conditions.

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Effect of Pancreozymin on Rat Pancreatic Enzyme Biosynthesis

Cited by 42 publications

References 17 publications

The effect of feeding on the secretion of pepsin, trypsin and chymotrypsin in the Atlantic salmon,Salmo salar L

The effect of feeding on the secretion of pepsin, trypsin and chymotrypsin in the Atlantic salmon,Salmo salar L

Regulation of Pancreatic α-Amylase in the Neonatal Mouse

Hormonal stimulation in the exocrine pancreas results in coordinate and anticoordinate regulation of protein synthesis.

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