Effect of intratympanic mitomycin C on the development of cholesteatoma and otitis media in rats

Abstract

Intratympanic mitomycin C was ineffective in preventing middle-ear cholesteatoma and otitis media in this rat model.

Single dose intratympanic mesna application inhibits propylene glycol induced cholesteatoma formation

“…The higher cholesteatoma formation rate compared with the study of Melo et al . can be attributed to the longer interval after intratympanic administration (12 weeks vs 10 weeks) 7 . The slightly higher mortality rate in the control group vs the mesna group (20 per cent vs 30 per cent) in the present study may be due to a lack of antibiotic prophylaxis.…”

“…According to this papillary ingrowth model, pathogenesis proceeds without retraction pockets or tympanic membrane perforation 8 . Although most studies have administered propylene glycol via the bulla, intratympanic administration can induce similar rates of cholesteatoma formation: bulla administration of propylene glycol is reported to cause cholesteatoma formation rates of 0–90 per cent, 5 – 12 while the only previous study to use intratympanic administration reported a rate of 63.6 per cent 7 . In the present study, cholesteatoma formation was achieved in seven out of eight ears (88 per cent) in the propylene glycol control group via intratympanic administration.…”

Does the Intratympanic Application of Mesna Prevent the Cholesteatoma? An Experimental Rats Study

“…The cholesteatoma prevalence seen in the control group of our study, %50, was evaluated to be in concordance with the studies in the literature (%33-90). 5 In our animal model study the decreases of the tympanic membrane thickness and the tympanic bulla mucosal thickness seen in the histopathological images between the control group and the experiment group were evaluated to be statistically significant. It was observed in the histopathological images that the cholesteatoma were mainly located at the tympanic bulla.…”

“…Melo et al showed in their experimental study that epidermal invasion occurred from the tympanic membrane to the tympanic bulla in the control group and study group. 5 In a study conducted on Winstar rats in which the effect of intratympanic single dose Mesna on cholestatoma was investigated, it was emphasized that single dose intratympanic Mesna prevented the formation of cholestatoma. 22 In our study, despite the intratympanic Mesna application applied for 4 times, no statistical significance was found, indicating that the formation of cholestatoma was completely prevented.…”

“…4 In the following years propylene glycol has been used in experimental studies in the development of otitis media and cholesteatoma because of its inflammatory property for the ear. [5][6][7] Sodium 2 -mercaptoethanesulfonate (C2 H 5 NaO 3S 2, Mesna ) is a synthetic sulfur compound that carries a thiol group. It breaks the disulfide bonds in a polypeptide chain with mucolysis.…”