Effect of interphase mixing on a water–organic solvent two‐liquid phase microbial system: ethanol fermentation

Bar, Raphael

doi:10.1002/jctb.280430106

Cited by 55 publications

(14 citation statements)

References 18 publications

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“…In the emulsion system, the hydrolytic reaction did not significantly proceed because of the strong inhibitory action of ester 1. It was assumed that the interfacial denaturation of lipase and/or the phase toxicity of 1 toward fungal cells appeared in the emulsion system [35,36]. In the two-liquid-phase system, the hydrolysis of ester 1 smoothly proceeded to afford 40 mg ml À1 of alcohol 2 for 8 days since n-decane might be play as a reservoir for 1 and/or 2.…”

Section: Discussionmentioning

confidence: 99%

Liquid-surface immobilization system and liquid–liquid interface bioreactor: Application to fungal hydrolysis

Oda¹,

Isshiki²

2007

Process Biochemistry

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Section: Discussionmentioning

confidence: 99%

Liquid-surface immobilization system and liquid–liquid interface bioreactor: Application to fungal hydrolysis

Oda¹,

Isshiki²

2007

Process Biochemistry

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“…The fermentation process consists of the conversion of glucose obtained from hydrolysis to bioethanol by microorganism media such as yeast, bacteria, and fungi [62,72,73]. Saccharomyces cerevisiae was used for fermentation assays [74][75][76].…”

Section: Fermentationmentioning

confidence: 99%

Review of Solvents Based on Biomass for Mitigation of Wax Paraffin in Indonesian Oilfield

et al. 2019

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This paper presents a review of the expectations and challenges of using biomass in the prevention and slowing of paraffin wax deposition that takes place during the crude oil production process. The inhibition of the deposition process involves the use of solvents from biomass that are generally available around the crude oil production field. The processes used to scale down the precipitation of wax include mixing crude oil with the manufacturer's solvent composed of toluene and xylene. The goal is to assess solvents sourced from biomass that are capable to slow down the wax deposition process. Wax appearance temperature is an important characteristic to evaluate the possible wax precipitation of a given fluid. Wax precipitation can be reduced by using some chemical additives, often called the pour point depressant. This additive is expected to be produced from local biomass which can compete with solvents currently produced on the market.

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“…Thus, although it was confirmed that the immobilized fungal spores could stably catalyze the reduction of benzil, which required a coenzyme in a harmless organic solvent, di-n-hexyl ether, the reduction activity of the immobilized spores gradually decreased in the more hydrophilic and toxic solvent, tert-butyl acetate. tert-Butyl acetate maybe extract water molecules from the alginate gel and spores and exhibits molecular toxicity, which is defined as microbial toxicity, due to the hydrophilic molecules dissolved in an aqueous phase 30,31 .…”

Section: A Novel Non-aqueous Bioconversion System Withmentioning

confidence: 99%

Novel, Non-aqueous Bioconversion Systems Using Fungal Spores

2018

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Two novel types of non-aqueous bioconversion systems using fungal spores, either adsorbed on the surface of a filter pad or entrapped in calcium alginate beads, were constructed and applied for a model reaction: reduction of benzil to benzoin by Aspergillus sojae NBRC 32074. The spores adsorbed on a filter pad catalyzed the reduction in some toxic organic solvents, such as methylcyclohexane (log P: 3.61) and din-butyl ether (3.21). For the relationship between the reduction activity and the log P value of the organic solvent, a highly positive correlation (R: 0.815) was observed. Surprisingly, the reduction proceeded in the more hydrophilic and toxic tert-butyl acetate (log P: 1.76). Glycerol was selected as the best hydride source. The higher the glycerol content, the more the benzoin was produced. While the production of benzil by spores was lower than that by mycelia in harmless di-n-hexyl ether (log P: 5.12), mycelia could not catalyze the reduction in the toxic tert-butyl acetate. In contrast, the spores entrapped in the calcium alginate beads could catalyze the reduction. Although the reduction by alginate-entrapped spores could be stably repeated 5 times in di-n-hexyl ether without a decline in the reduction activity, it was observed that the reduction activity of the spores gradually decreased after repeated reduction in tert-butyl acetate.

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Effect of interphase mixing on a water–organic solvent two‐liquid phase microbial system: ethanol fermentation

Abstract: Ethanol fermentation bywere correlated with 'log P' values of the solvents. Full activity was observed at log P > 5 and almost none at log P < 4 .

Cited by 55 publications

References 18 publications

Liquid-surface immobilization system and liquid–liquid interface bioreactor: Application to fungal hydrolysis

Liquid-surface immobilization system and liquid–liquid interface bioreactor: Application to fungal hydrolysis

Review of Solvents Based on Biomass for Mitigation of Wax Paraffin in Indonesian Oilfield

Novel, Non-aqueous Bioconversion Systems Using Fungal Spores

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