Liquid-surface immobilization system and liquid–liquid interface bioreactor: Application to fungal hydrolysis

Production of Valuable Lipophilic Compounds by Using Three Types of Interface Bioprocesses: Solid–Liquid Interface Bioreactor, Liquid–Liquid Interface Bioreactor, and Extractive Liquid-Surface Immobilization System

“…As shown in Fig. 8, the L/L-IBR showed the most efficient performance among the four studied systems; it yielded 107 g/L of 2-ethyl-1-hexanol after 8 days of cultivation 71 . Later, the performance of L/L-IBR was attributed to the activating lipase of A. coerulea, which used Ca 2 from the CaCO 3 -coated MFL-80GCA MS 75 , similar to the other lipases 76 .…”

“…L/L-IBR was first used for the hydrolysis of 2-ethylhexyl acetate into 2-ethyl-1-hexanol by Absidia coerulea NBRC 4423 71 . The type culture fungus was screened from approximately 400 strains using the S/L-IBR, to yield 51.1 g/L of 2-ethyl-1-hexanol from a 50 w/v solution of 2-ethylhexyl acetate in n-decane in 5 days.…”

Discovery of secondary metabolites in an extractive liquid-surface immobilization system and its application to high-throughput interfacial screening of antibiotic-producing fungi

“…The organic phase in the liquid-liquid interface bioreactor also has an extractant and a reservoir of toxic substrate and/or product to hold very high level of various hydrophobic conversion products. 17,[20][21][22][23][24][25] Thus, the spontaneous extraction of hydrophobic secondary metabolites maybe contribute to peculiarity of secondary metabolite profile of the Ext-LSI-Org. Next, it is also assumed that high solubility of oxygen in the organic phase affects the peculiar metabolite profile in the Ext-LSI-Org.…”

Relationship between Interfacial Hydrophobicity and Hydroxylation Activity of Fungal Cells Located on an Organic–Aqueous Interface

“…A Liquid-liquid interface bioreactor (L-L IBR), which consists of a hydrophobic organic solvent (an upper phase), a layer of fungal cellballooned polyacrylonitrile microsphere (diameter, 20-40 μm; density, 0.03-0.20; a middle phase), and a liquid medium (a lower phase), is a unique and effective cultivation system for the microbial transformation with fungi [1]. The system has some interesting and practically important characteristics, i.e., alleviation of toxicity of poisonous substrate and/or product solubilized in the organic phase, excellent productivity of valuable hydrophobic chemicals, efficient supply of oxygen from the organic phase to fungal cells, control and management of pH and nutrients in the liquid medium, depression of catabolize repression caused by easily metabolizable carbon sources [2], and easy recovery of product without troublesome solvent extraction.…”