Effect of interleukin‐1β and lipoxin A₄ in human endometriotic stromal cells: Proteomic analysis

Abstract

Aim: Lipoxin A 4 (LXA 4 ) can function as an endogenous 'breaking signal' in inflammation and plays an important role in the progression of endometriosis. The proteome responses to interleukin-1β (IL-1β) or LXA 4 in human endometriotic stromal cells (ESC) are not well understood. Methods: In this study, primary ESC were cultured from ovarian endometriosis tissue. Three groups were established: the control group; the IL-1β stimulation group; and the IL-1β and LXA 4 incubation group. Proteins were assessed on 2-D polyacrylamide gel electrophoresis (2D-PAGE), and differentially expressed protein spots were further identified on matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MALDI-TOF-MS). Wound healing and transwell assays were performed to assess the migration and invasion of ESC after treatment. Results: In total, 40 differentially expressed protein spots were identified successfully on MALDI-TOF-MS. The proteins identified were related to cell structure, metabolism, signal transduction, protein synthesis and membrane structure, processes that may be involved in the development of endometriosis. Vinculin and IL-4 were further analyzed on western blot and quantitative real-time polymerase chain reaction. Moreover, LXA 4 could suppress the migration and invasion of ESC induced by IL-1β. Conclusion: LXA 4 may inhibit the progression of endometriosis partly by lowering or raising the effect of IL-1β, mediated via some inflammation-related proteins (e.g. vinculin) and immune response-related protein (e.g. IL-4) in vitro.

The Inflammatory Role of Pro-Resolving Mediators in Endometriosis: An Integrative Review

“…In these studies, patients were at least 3–6 months without hormonal or anti-inflammatory treatment. ESCs were treated in cell culture with the mediator under investigation, and the evaluation was carried out through several types of laboratory analysis [ 7 , 19 , 30 , 31 , 33 , 34 , 35 ]. Rasheed et al analyzed in vitro halogenic stem cells by adding serum from patients diagnosed with endometriosis to evaluate cell differentiation and the expression of ANXA1 [ 37 ].…”

“…Further, LXA4 inhibited COX-2 expression induced by IL-1β in ESCs through the FPR2/ALX modulation. According to Wu et al in proteomic analysis, LXA4 can suppress proteins that facilitate IL-1β-induced migration and invasion in ESC [ 34 ].…”

How Do Microorganisms Influence the Development of Endometriosis? Participation of Genital, Intestinal and Oral Microbiota in Metabolic Regulation and Immunopathogenesis of Endometriosis

“…Tumor necrosis factor alpha (TNF-α) also belongs to the group of pro-inflammatory cytokines. Several studies have shown that its serum concentration in patients with endometriosis may be elevated [ 93 ]. The development and progression of endometriosis is also influenced by interactions between the two groups of cytokines.…”