The diversified microstructure and complex composition of cast iron affect its friction and wear properties. In this work, three kinds of cylinder liner cast iron with different graphite morphologies were selected to analyse the friction and wear properties. Microstructure effects on tribological properties during friction were investigated by finite element simulation and pin-on-disc dry friction test. Wear mechanisms were studied using scanning electron microscopy and X-ray photoelectron spectroscopy. The results show that graphite bears a considerable strain value during the friction process, which is prone to wear and fall off, and the fallen graphite can reduce the friction coefficient. Ferrite around graphite can buffer stress, but it also can reduce wear resistance. And the more significant the lamellar spacing of pearlite, the more abrasive and adhesive effect.