Effect of inoculum and sulfide type on simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine slurry and microbial mechanism

Abstract

Four reactors were initiated to study the effect of inoculum and sulfide type on the simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine slurry (Ssu-Nir) process. Anaerobic sludge, aerobic sludge, and water were used as inocula, and Na2S and biogas were used as a sulfide substrate, respectively. Additionally, 454 pyrosequencing of the 16S rRNA gene was used to explore the bacterial diversity. The results showed that sulfur-oxidizing bacteria (Thiobacillus, 42.2-84.4 %) were dominant in Ssu-Nir process and led to the excellent performance. Aerobic sludge was more suitable for inoculation of the Ssu-Nir process because it is better for rapidly enriching dominant sulfur-oxidizing bacteria (Thiobacillus, 54.4 %), denitrifying sulfur-oxidizing bacteria (40.0 %) and denitrifiers (23.9 %). Lower S(2-) removal efficiency (72.6 %) and NO3 (-) removal efficiency (<90 %) of the Ssu-Nir process were obtained using biogas as a sulfide substrate than when Na2S was used. For the Ssu-Nir process with biogas as the sulfide substrate, limiting H2S absorption caused a high relative abundance of sulfur-oxidizing bacteria, Thiobacillus (84.8 %) and Thiobacillus sayanicus (39.6 %), which in turn led to low relative abundance of denitrifiers (1.6 %) and denitrifying sulfur-oxidizing bacteria (24.4 %), low NO3 (-) removal efficiency, and eventually poor performance.

Biological removal of gas‐phase H2S in hollow fibre membrane bioreactors

“…The relative abundance of functional microorganisms (that is, ASOB) plays a key role in the biological desulphurization process 4,58 . ASOB communities utilize H 2 S and CO 2 as their energy and carbon source, respectively 56 .…”

“…Activated sludge is commonly employed as inoculum during the start-up of different bioreactor configurations (e.g., biofilter or bioscrubber) for treating H 2 S. 57 The relative abundance of functional microorganisms (that is, ASOB) plays a key role in the biological desulphurization process. 4,58 ASOB communities utilize H 2 S and CO 2 as their energy and carbon source, respectively. 56 The most abundant phylum in the HFMB, Proteobacteria (Fig.…”

Efektivitas Variasi Umpan Pada Fly Trap Dalam Pengendalian Kepadatan Lalat

“…Lalat memiliki indra penciuman yang sangat peka pada antena dan palpus, sehingga mampu mencium bau yang menguap dalam suhu kamar, dan bahan yang membusuk (Nadeak et al, 2017;Tanjung, 2016). Gas sulfida yang dihasilkan dari proses pembusukan merupakan penarik bagi lalat (Ristiati et al, 2018;Shane, Montrose, & Harrington, 1985;Wang et al, 2015).…”