Effect of inoculation with Lactobacillus buchneri LB1819 and Lactococcus lactis O224 on fermentation and mycotoxin production in maize silage compacted at different densities

The effect of mixed bacterial inoculant on the microbial population and aerobic stability of lucerne and maize balage

“…Conversely, we have observed that silage inoculant had a positive effect on aerobic stability by delaying aerobic deterioration time. The present results are in agreement with several previous studies, where inoculation with L. buchneri and L. lactis improved aerobic stability in various silages (Witt et al, 2015;Copani et al, 2017;Gallo et al, 2018). The results of the present study confirm that applying bacterial inoculant containing L. buchneri and L. lactis can efficiently control yeast and moulds growth and improve aerobic stability of maize and lucerne ensiled in big bales.…”

Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock

“…Importantly, L. buchneri increased the aerobic stability of the silage as well (Cavallarin et al, 2011). Inoculation of corn silage with a combined inoculant of L. buchneri and Lactococcus lactis improved the aerobic stability of the silage, and the higher silage density increased the stability further (Gallo et al, 2018). The interaction of L. buchneri, L. reuteri, L. plantarum, and L. fermentum strains reduced the AFB 1 level, improved the stability and, furthermore, the microbiological and chemical purity of maize silage (Zielińska and Fabiszewska, 2018).…”

“…Leuconostoc, and Pediococcus (P. pentosaceus) genera can inhibit or can even prevent completely the growths of various mycotoxigenic molds and their mycotoxin productions as well (Dalié et al, 2010;Cavallarin et al, 2011;Queiroz et al, 2012;Dogi et al, 2013;Ahlberg et al, 2015;Ma et al, 2017;Gallo et al, 2018;Zielińska and Fabiszewska, 2018;Ferrero et al, 2019). It is noteworthy that there is a wide spectrum of environmental factors which influence the antifungal activity of LAB, including the type of the matrix and culture medium, the availability of nutritional compounds, the incubation time and temperature (Dalié et al, 2010;Ahlberg et al, 2015;Leyva Salas et al, 2017).…”

Co-Occurrence of Regulated and Emerging Mycotoxins in Corn Silage: Relationships with Fermentation Quality and Bacterial Communities

“…The water mixture was then filtered through a gauze and an aliquot (10 mL approximately) of the resulting solution was centrifuged at 4500× g for 15 min. The liquid supernatant was micro-filtered on a 0.45 µm syringe filter and an aliquot of micro-filtered supernatant (1.5 mL) was transferred in a vial and added with 10 µL of internal standard solution (pivalic acid at 1.5% in distilled water) for VFA gas-chromatographic analysis [ 76 ]. The analysis was carried out using a 2025 GC with Flame Ionization Detector (FID) system (Shimadzu S.r.l., Milano, Italy) equipped with auto-sampler (AOC-20i Shimadzu S.r.l., Milano, Italy) and capillary column DB-FFAP (30 m 0.250 mm; 0.25 µm; Agilent Technologies S.p.A., Milano, Italy).…”