EFFECT OF INOCULATION WITH ARBUSCULAR MYCORRHIZAL FUNGUS Rhizophagus irregularis ON AUXIN CONTENT IN HIGHLY MYCOTROPHIC BLACK MEDICK UNDER LOW PHOSPHORUS IN SOIL

Yurkov, Andrey P.; Веселова, С. В.; Jacobi, Lidija M.; Степанова, Галина; Kudoyarova, G. R.; Шишова, М. Ф.

doi:10.15389/agrobiology.2017.4.830eng

Cited by 6 publications

(11 citation statements)

References 32 publications

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“…Black medick (Medicago lupulina L. var. vulgaris Koch, annual) is known to establish symbiosis with the strain RCAM00320 of AM fungus Rhizophagus irregularis (previously known as Glomus intraradices Shenck&Smith; strain CIAM8 from the collection of the All-Russian Research Institute for Agricultural Microbiology; Yurkov et al 2010). The selected MlS-1 line from cultivar-population VIK32 was used in the present study.…”

Section: Methodsmentioning

confidence: 99%

“…The substrate for growing (air-dry soilsand mixture in 2:1) was autoclaved twice. Plants were grown in a UV-sterilized light chamber with inactive ventilation under standard conditions (luminous flux at ~1500-1700 lm, a 16h/8h day/ night cycle, temperature of ~24-26°C, ambient air humidity ~60-65%, plant watering up to 0.6 of saturated water content, Yurkov et al 2010). Four seedlings were planted per 1 pot, filled with soil-sand mixture (210 g).…”

Section: Methodsmentioning

confidence: 99%

“…Four seedlings were planted per 1 pot, filled with soil-sand mixture (210 g). Agrochemical soil parameters were described earlier (Yurkov et al 2010), P level was 17 mg/kg. Light microscopy.…”

Section: Methodsmentioning

confidence: 99%

“…AM-fungi might consume up to 20% of host plant photosynthates but simultaneously enhance plant growth (Smith and Read 2008). This intensification commonly depends on the alteration in plant hormone balance, which has become an object of special interest in recent years (Fusconi 2014, Boivin et al 2016, Yurkov et al 2017.…”

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confidence: 99%

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The effect of inoculation with arbuscular mycorrhizal fungus Rhizophagus irregularis on cytokinin content in a highly mycotrophic Medicago lupulina line under low phosphorus level in the soil

Andrey¹,

Веселова²,

Lidia³

et al. 2017

Plant Soil Environ.

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Arbuscular mycorrhizae (AM) is a mutualistic nonspecific symbiosis formed by most land plant families (92%) with fungi represented by the Glomeromycota phylum. This type of symbiosis is The study is focused on the elucidation of the role of cytokinins (CKs, zeatin and zeatin riboside) in the development of effective arbuscular mycorrhiza (AM) symbiosis with Medicago lupulina. An important mechanism involved in the regulation of host plant growth is supposed to be linked to the modulation of plant hormone balance. The data obtained revealed the formation of an effective AM-symbiosis (M. lupulina + Rhizophagus irregularis) under phosphorus-deficiency. At the shooting stage (35 th day after sowing), it is characterized by a decrease in the root:shoot ratio, the lowering in arbuscules and vesicle abundances, but an increase in the intensity of mycelium development. Mycorrhized plants differed from the control ones by higher CK levels in both roots and leaves. Zeatin and zeatin riboside concentration exhibited uneven alterations over time. A role of mycelium in the modulation of CK balance has been discussed.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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The effect of inoculation with arbuscular mycorrhizal fungus Rhizophagus irregularis on cytokinin content in a highly mycotrophic Medicago lupulina line under low phosphorus level in the soil

Andrey¹,

Веселова²,

Lidia³

et al. 2017

Plant Soil Environ.

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“…A.P. Yurkov et al earlier described the plant cultivation protocol details using phytobox [70,73]. Plants were planted with two seedlings in one vessel filled with a soil-sand mixture weighing 210 g. Agrochemical soil characteristics: sod-podzolic loam-poor soil with a low P content; P 2 O 5 and K 2 O contents (calculated using the Kirsanov reaction) were 23 mg and 78 mg per 1 kg of soil, respectively; organic matter content was 3.64%; pH KCl was 6.44 and pH H2O was 7.28.…”

Section: Microvegetation Methodsmentioning

confidence: 99%

AM-Induced Alteration in the Expression of Genes, Encoding Phosphorus Transporters and Enzymes of Carbohydrate Metabolism in Medicago lupulina

Yurkov

Kryukov

Gorbunova

et al. 2020

Plants

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Plant–microbe interactions, including those of arbuscular mycorrhiza (AM), have been investigated for a wide spectrum of model plants. The present study focuses on an analysis of gene expression that encodes phosphate and sugar transporters and carbohydrate metabolic enzymes in a new model plant, the highly mycotrophic Medicago lupulina MLS-1 line under conditions of phosphorus deficiency and inoculation with Rhizophagus irregularis. Expression profiles were detected by RT-PCR at six plant stages of development (second leaf, third leaf, shooting, axillary shoot branching initiation, axillary shoot branching, flowering initiation). In comparison to control (without AM), the variant with AM inoculation exhibited a significant elevation of transcription levels of carbohydrate metabolic enzymes (MlSUS, MlHXK1) and sucrose transporters (MlSUC4) in M. lupulina leaves at the shooting stage. We suggest that this leads to a significant increase in the frequency of AM infection, an abundance of mycelium in roots and an increase in AM efficiency (which is calculated by the fresh weight of aerial parts and roots at the axillary shoot branching initiation stage). In roots, the specificity of MlPT4 and MlATP1 gene expressions were revealed for effective AM symbiosis. The level of MlPT4 transcripts in AM roots increased more than tenfold in comparison to that of non-specific MlPT1 and MlPT2. For the first time, MlPT1 expression was shown to increase sharply against MlPT2 in M. lupulina roots without AM at the shooting initiation stage. A significant increase in MlRUB expression was revealed at late stages in the host plant’s development, during axillary shoot branching and flowering initiation. The opposite changes characterized MlHXK1 expression. Alteration in MlHXK1 gene transcription was the same, but was more pronounced in roots. The obtained results indicate the importance of genes that encode phosphate transporters and the enzymes of carbohydrate metabolism for effective AM development at the shooting stage in the host plant.

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HORMONE BALANCE AND SHOOT GROWTH IN WHEAT(Triticum durum Desf.) PLANTS AS INFLUENCED BY SODIUM HUMATES OF THE GRANULATED ORGANIC FERTILIZER

Nazarov

2020

S-h. biol.

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Гуминовые вещества образуются в почве в процессе разложения органических остатков и способны стимулировать рост и повышать продуктивность растений. В растениеводстве широко применяют наиболее растворимые препараты на основе гуматов натрия и калия, которые предположительно обладают гормоноподобной активностью. Однако механизм влияния гуминовых веществ на физиологические и биохимические процессы в растениях до сих пор не до конца понятен. Гуминовые вещества могут оказывать стимулирующее действие в очень низких концентрациях, что определяет требования к адресности и режиму их поступления в растения. Этим требованиям соответствует размещение удобрения в непосредственной близости от семян. Ранее было показано положительное влияние инкрустации гранул нитрофоски гуматом натрия (ГН) на урожайность яровой твердой пшеницы. В настоящем исследовании впервые представлены данные об изменении ростовых процессов и гормонального баланса у растений пшеницы (сорт Башкирская 27) при применении ГН в составе органоминерального удобрения (ОМУ). Гранулы удобрений без ГН и содержащие 1,25½10 2 %, 2,5½10 2 % и 5½10 2 % гуминового препарата (по массе гранул) размещали на расстоянии 2-3 см от высаженных в почву 1-суточных проростков пшеницы. ОМУ получали на основе отхода птицеводства (куриный помет) и доломита, ГН-экстракцией из бурого угля. Контролем служили растения, которые не получали ни гуматов, ни дополнительного питания в виде ОМУ. С 3-х сут после посадки измеряли длину листьев и эвапотранспирацию (по уменьшению массы сосудов с растениями). Образцы листьев для определения фитогормонов с помощью иммуноферментного анализа собирали на 9-е сут, площадь листьев, массу растений и содержание общего азота определяли на 21-е сут после посадки. Органоминеральные гранулы без ГН стимулировали удлинение листьев, что проявлялось в достоверно большей их длине по сравнению с контролем (42, 156, 187 и 274 против 47, 167, 199 и 294 мм, р  0,05), зарегистрированной для первого листа через 3, 6 и 8, для второго-через 14 сут после посадки. При этом увеличение длины листьев не зависело от дозы ОМУ, повышение которой не приводило к достоверному изменению анализируемого показателя. Добавление гуминовых веществ к гранулам ОМУ дополнительно стимулировало удлинение листьев, что наиболее ярко проявлялось в варианте с промежуточной концентрации ГН (2,5½10 2 %), при которой за время наблюдения (через 3, 6, 8 и 14 сут после посадки) была выявлена достоверная прибавка длины листьев по сравнению с эффектом от применения ОМУ без ГН (6,0; 11,0; 13,0 мм прибавки по первому листу и 9,4; 9,0; 22,0-по второму, р  0,05). В отличие от ОМУ без ГН, применение которых не изменяло массу побега и площадь листьев, сочетание ОМУ с ГН в промежуточной концентрации достоверно увеличивало массу побегов (с 538 до 583 мг, р  0,05). Площадь листьев достоверно превышала контроль в вариантах с промежуточной и максимальной концентрациями ГН (на 385 и 283 мм 2 , р  0,05). Таким образом, добавление ГН к ОМУ повышало эффективность воздействия органоминерального удобрения на ростовые по...

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EFFECT OF INOCULATION WITH ARBUSCULAR MYCORRHIZAL FUNGUS Rhizophagus irregularis ON AUXIN CONTENT IN HIGHLY MYCOTROPHIC BLACK MEDICK UNDER LOW PHOSPHORUS IN SOIL

Cited by 6 publications

References 32 publications

The effect of inoculation with arbuscular mycorrhizal fungus Rhizophagus irregularis on cytokinin content in a highly mycotrophic Medicago lupulina line under low phosphorus level in the soil

The effect of inoculation with arbuscular mycorrhizal fungus Rhizophagus irregularis on cytokinin content in a highly mycotrophic Medicago lupulina line under low phosphorus level in the soil

AM-Induced Alteration in the Expression of Genes, Encoding Phosphorus Transporters and Enzymes of Carbohydrate Metabolism in Medicago lupulina

HORMONE BALANCE AND SHOOT GROWTH IN WHEAT(Triticum durum Desf.) PLANTS AS INFLUENCED BY SODIUM HUMATES OF THE GRANULATED ORGANIC FERTILIZER

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