Effect of Inoculation of Pectinolytic Mixed Bacterial Culture on Improvement of Ribbon Retting of Jute and Kenaf

Banik, S.; Basak, M. K.; Sil, S. C.

doi:10.1300/j395v04n02_03

Cited by 26 publications

(10 citation statements)

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“…Moreover, retting water varies from place to place with respect to its physico-chemical, microbial and biochemical properties which affect fibre quality 21,22 . Due to scarcity of water, farmers are compelled to use water bodies repeatedly for retting, leading to poor quality jute fibre production 21,23 . Acute shortage of water and the environmental pollution created from conventional system of retting has demanded for improving the retting process.…”

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confidence: 99%

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Hasan

Aktar

Kabir

et al. 2020

Sci Rep

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Jute fibre is the second most important fibre next to cotton. It is obtained from the bark of plant through microbial retting process. Here we report optimized microbial retting protocol that can lower retting period and produce high fibre quality. A total of 451 bacterial colonies have been isolated from five jute retting water samples in Bangladesh. Higher pectinolytic bacterial isolates were predominant in the later stage of jute retting. Out of these, 168 isolates have been screened by both semi-quantitative and quantitative pectinase, xylanase and cellulase enzyme assay. Among them, 144 isolates have been selected on the basis of extra cellular enzyme activity of these three enzymes. 16 s ribosomal gene sequencing analysis identified 2 phyla-Firmicutis (80.55%) and Proteobacteria (19.45%). To check the synergistic and antagonistic effect 10 selected isolates were tested in 167 different combinations. Three best combinations were identified that lowered retting period from 18-21 days to 10 days producing high quality fibre in both laboratory and field trial. This improved retting technology can be adopted in industrial scale for the production of quality jute fibre in a controlled condition in reduced water quantity without polluting the environment.Jute, second most important natural fibre after cotton, is cultivated in East Asia and some parts of Latin America 1-3 . Jute is a bast or phloem fibre in the bark of stems, cemented together by pectin and gummy substances 4 . Commercial extraction of jute fibre is water based microbiological retting where jute bundles are submerged into slow running river water and subjected to decomposition of pectin, hemicelluloses, and other mucilaginous substances 2,5 . In this process, pectin is depolymerized by pectinases, primarily comprising four enzymes: Polygalacturonase (PG), Pectin Lyase (PNL), Pectate lyase and Pectin esterase. However, PG 6 , and PNL 7 are primary retting enzymes. In addition, xylanase makes jute fibre softer by selective removal of non-fibrous hemicelluloses without affecting strength of cellulosic fibre. Pectinolytic microorganisms having xylanase activity devoid of cellulase activity is an additional beneficial aspect to improve fibre quality 8 . The quality of fibre is largely determined by the efficiency of retting process 1,2,9,10 and various factors are responsible for proper retting as well as improved fibre quality. Most promising water based microbiological retting process mostly involves bacteria along with various fungi, protozoa, algae and diatoms 10-13 . Main aerobic retting bacteria belong to genus Bacillus viz., B. subtilis, B. polymyxa, B. mesentericus, B.pumilus, B. cereus, B. megaterium and B. macerans, initiate retting 14-18 along with large numbers of gram-negative genera such as Erwinia and Pseudomonas 9,19 . At the later stage of retting some anaerobic bacteria from genus Clostridium -Clostridium acetobutylicum, Clostridium stercorarium, Clostridium tertium come to carry on the retting process 20 . As microorganisms are the ma...

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confidence: 99%

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Hasan

Aktar

Kabir

et al. 2020

Sci Rep

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“…Although jute retting is better in slow moving soft water, only 10 % of jute growers of India have access to this and the remaining 90% ret their harvest in stagnant water bodies like lakes, ditches and ponds. Use of microbial inoculants to hasten the jute retting in stagnant water retting has been reported by several workers (Banik et al, 2007;Das et al, 2012;Das et al, 2015;Das et al, 2018). The major drawback in using the carrier based microbial inoculants either as biofertilizer/ biopesticides or bioformulation is their short shelf life, as the microbial cells cannot withstand environmental stress for prolonged period.…”

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confidence: 99%

Use of bacterial endospore with longer shelf-life in improved retting of jute

Chattopadhyay¹,

Majumdar²,

Mazumdar³

et al. 2019

JEB

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“…Chemical semi-degumming is inapplicable to jute fibers, which are short and existing as bundles. Therefore, the imperfect degumming technology restricts the development of the jute industry (Ahmed and Akhter 2001 ; Banik et al 2007 ). Bio-degumming catalyzes and degrades non-cellulosic components (including pectin, hemicelluloses, and lignin) in jute bast by using enzymes secreted by microorganisms, resulting in cellulose extraction (Kozlowski et al 2006 ).…”

Section: Introductionmentioning

confidence: 99%

“…Bio-degumming catalyzes and degrades non-cellulosic components (including pectin, hemicelluloses, and lignin) in jute bast by using enzymes secreted by microorganisms, resulting in cellulose extraction (Kozlowski et al 2006 ). Bio-degumming is a clean, environmentally friendly, highly efficient, high-quality, and low-cost method and is becoming the leading jute degumming technology (Banik et al 2007 ; Biswas et al 2013 ; Liu and Peng 2004 ). Microbial degumming involves enzyme degumming and bacterial degumming.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the immature enzyme mixing technology and the costly enzymes restrict the applications of enzyme degumming. For bacterial degumming, some researchers have studied the screening and community structure of degumming bacterial strains of jute (Banik et al 2007 ; Das et al 2012 ; Haque et al 2003 ; Munshi and Chattoo 2008 ). A mixed bacterial retting culture was inoculated during ribbon retting of jute, and most of the defects arising from conventional retting could be overcome by ribbon retting (Banik et al 2003 ).…”

Section: Introductionmentioning

confidence: 99%

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Bio-degumming technology of jute bast by Pectobacterium sp. DCE-01

et al. 2016

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Among industrial fiber crops, jute is ranked second to cotton in terms of yield and planting area worldwide. The traditional water retting and chemical semi-degumming methods restrict the development of the jute industry. Jute fiber can be extracted from jute bast through mechanical rolling (preprocessing), culture of bacteria, soaking fermentation (liquor ratio = 10, inoculum size = 1 %, temperature = 35 °C, and time = 15 h), inactivation, washing, and drying. Pectobacterium sp. DCE-01 secretes key degumming enzymes: pectinase, mannase, and xylanase, which match well the main non-cellulosic components of jute bast. Compared with the traditional water retting degumming, the bio-degumming cycle is shortened from more than 10 days to 15 h. The proposed bio-degumming achieved higher efficiency and lower pollution than water retting and chemical semi-degumming.

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Effect of Inoculation of Pectinolytic Mixed Bacterial Culture on Improvement of Ribbon Retting of Jute and Kenaf

Cited by 26 publications

References 1 publication

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Use of bacterial endospore with longer shelf-life in improved retting of jute

Bio-degumming technology of jute bast by Pectobacterium sp. DCE-01

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