Effect of inhibition of Helicobacter pylori urease activity by acetohydroxamic acid on serum gastrin in duodenal ulcer subjects.

Abstract

The mechanism of the hypergastrinaemia associated with Helicobacterpylori infection is unknown. It may be an effect of the ammonia produced by the bacterium near the antral epithelial surface. We In an attempt to elucidate the mechanism of the hypergastrinaemia associated with H pylori infection, we have examined the effect of inhibiting the bacterium's urease activity and ammonia production on serum gastrin in duodenal ulcer patients. Patients and methods STUDIES IN PATIENTS WITH H PYLORI INFECTIONSix patients confirmed endoscopically to have duodenal ulceration within the previous year but currently in clinical remission were studied. Their median age was 39 years (range 26-52) and three were women. In each patient, an antral biopsy specimen obtained endoscopically within the preceding three months had shown gastritis associated with H pylori like organisms.The patients reported fasted and a venous blood sample was removed at 8 am for gastrin determination. Immediately after this they drank 50 ml water and further blood samples were taken at 30 minute intervals for two hours. At 10 am they took a standard meal consisting of two beef cubes (OXO Ltd, Croydon, England) dissolved in 200 ml water at 50°C. Further blood samples were taken at 10 minute intervals for 70 minutes and a final one at 90 minutes after the OXO drink. Immediately after this sample a "C urea breath test was performed to measure H pyloni urease activity. For this they drank 250 ml Ensure Plus (Abbott Laboratories, England) to delay gastric emptying, followed by 0 4 MBq 14C urea (Amersham International) in 25 ml water. Breath samples for 14C-Co2 analysis were obtained at 10 minute irtervals for 90 minutes.

Effect of Helicobacter pylori on Parietal Cell Sensitivity to Pentagastrin in Duodenal Ulcer Subjects

“…(13) noted that healthy volunteers with H . pylori had increased basal and meal-stimulated gastrin concentrations compared with uninfected volunteers, yet there was no difference between the two groups with regard to (5)(6)(7). basal, maximal, or meal-stimulated acid outputs.…”

Inhibition of Helicobacter pylori growth and its colonization factors by Parthenium hysterophorus extracts

“…As it can be seen in (Ibrar et al, 2013). Furthermore, in a clinical trial, in which 750 mg of AHA was administrated orally in a single dose to H. pylori infected patients, a temporary inhibitory effect on urease and ammonia production was observed, but it was not enough to clear the infection (El Nujumi et al, 1991). Then, the search for novel urease inhibitors, more stable and safer still stands.…”

Helicobacter pylori and gut hormones

“…16 Inhibition of urease by acetohydroxamic acid or bismuth plus antibiotics did not decrease gastrin release in a short-term experiment. 46 In contrast, long-term exposure of the rat antral mucosa to elevated levels of ammonia in the gastric juice induced G-cell hyperfunction in rats. 47 and monochloramine (NH 2 Cl) was shown to be a more potent stimulant of gastrin release and acid secretion than ammonia itself.…”