Effect of increasing zinc sulphate concentration during in vitro maturation of bovine oocytes

Picco, Sebastián Julio; Anchordoquy, Juan Mateo; Matos, D.G. de; Seoane, Analía Isabel; Mattioli, Guillermo Alberto; Errecalde, Ana Lı́a; Furnus, Cecilia Cristina

doi:10.1016/j.theriogenology.2010.05.015

Cited by 44 publications

(37 citation statements)

References 45 publications

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“…Knowledge of zinc metabolism and action in the oocyte, cumulus cells, and early embryo could lead to improved in vitro maturation procedures for human in vitro fertilization, which are very inefficient (4 -27% clinical pregnancy/transfer) (75)(76)(77). In cattle, zinc supplementation of in vitro maturation medium improves blastocyst production from 17 to 30% (78). Also, large influx and efflux of zinc ions during fertilization and in early zygotes suggests a role for zinc as a signaling molecule during early development (79).…”

Section: In Vivo Zinc Deficiency Causes Ovulatory Defectsmentioning

confidence: 99%

Zinc Depletion Causes Multiple Defects in Ovarian Function during the Periovulatory Period in Mice

Tian

Diaz

2012

Endocrinology

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Shortly before ovulation, the oocyte acquires developmental competence and granulosa cells undergo tremendous changes including cumulus expansion and luteinization. Zinc is emerging as a key regulator of meiosis in vitro, but a complete understanding of zinc-mediated effects during the periovulatory period is lacking. The present study uncovers the previously unknown role of zinc in maintaining meiotic arrest before ovulation. A zinc chelator [N,N,N',N'-tetrakis (2-pyridylmethyl) ethylenediamine (TPEN)] caused premature germinal vesicle breakdown and associated spindle defects in denuded oocytes even in the presence of a phosphodiesterase 3A inhibitor (milrinone). TPEN also potently blocked cumulus expansion by blocking induction of expansion-related transcripts Has2, Ptx3, Ptgs2, and Tnfaip6 mRNA. Both meiotic arrest and cumulus expansion were rescued by exogenous zinc. Lack of cumulus expansion is due to an almost complete suppression of phospho-Sma- and Mad-related protein 2/3 signaling. Consistent with a decrease in phospho-Sma- and Mad-related protein 2/3 signaling, TPEN also decreased cumulus transcripts (Ar and Slc38a3) and caused a surprising increase in mural transcripts (Lhcgr and Cyp11a1) in cumulus cells. In vivo, feeding a zinc-deficient diet for 10 d completely blocked ovulation and compromised cumulus expansion. However, 42.5% of oocytes had prematurely resumed meiosis before human chorionic gonadotropin injection, underscoring the importance of zinc before ovulation. A more acute 3-d treatment with a zinc-deficient diet did not block ovulation but did increase the number of oocytes trapped in luteinizing follicles. Moreover, 23% of ovulated oocytes did not reach metaphase II due to severe spindle defects. Thus, acute zinc deficiency causes profound defects during the periovulatory period with consequences for oocyte maturation, cumulus expansion, and ovulation.

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Section: In Vivo Zinc Deficiency Causes Ovulatory Defectsmentioning

confidence: 99%

Zinc Depletion Causes Multiple Defects in Ovarian Function during the Periovulatory Period in Mice

Tian

Diaz

2012

Endocrinology

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“…Adequate Zn concentration improved competence of bovine oocytes manifested by increased blastocyst rates (Picco et al, 2010). We observed that apoptosis in CC was significantly low in cells cultured with Zn.…”

Section: Discussionmentioning

confidence: 61%

Analysis of apoptosis and DNA damage in bovine cumulus cells after exposurein vitroto different zinc concentrations

et al. 2011

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The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 μg/ml (Zn1); 1.1 μg/ml (Zn2) and 1.5 μg/ml (Zn3)] to the culture medium was evaluated by comet assay. In addition, early apoptosis was analysed by annexin staining assay. CCs treated with Zn showed a significant decrease in the DNA damage in a dose-dependent manner. Comet assay analysed for TM (tail moment) was significantly higher in cells cultured without Zn (control, P<0.01) with respect to cells treated with Zn (control: 5.24±16.05; Zn1: 1.13±5.31; Zn2: 0.10±0.36; Zn3: 0.017±0.06). All treatments were statistically different from the control (P = 0.014 for Zn1; P<0.01 for Zn2 and Zn3). The frequency of apoptotic cells was higher in the control group (control: 0.142±0.07; Zn1: 0.109±0.0328; Zn2:0.102±0.013; Zn3: 0.0577±0.019). Statistical differences were found between control and Zn1 (P = 0.0308), control and Zn2 (P = 0.0077), control and Zn3 (P<0.0001), Zn1 and Zn3 (P<0.001) and Zn2 and Zn3 (P = 0.0004). No differences were found between Zn1 and Zn2. In conclusion, low Zn concentrations increase DNA damage and apoptosis in CCs cultured in vitro. However, adequate Zn concentrations 'protect' the integrity of DNA molecule and diminish the percentage of apoptotic CC.

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“…También se han llevado a cabo estudios in vitro, con animales y humanos que analizan la influencia del zinc en los resultados del embarazo y la fertilidad, encontrando todos ellos una asociación positiva entre función reproductora y este nutriente [31][32][33][34][35][36][37][38][39] , debido principalmente a su implicación en diversas funciones biológicas y rutas metabólicas 31 . Determinados autores mostraron que la deficiencia de zinc, aunque es poco frecuente en países desarrollados, podía ocasionar tanto alteraciones en el feto como malformaciones congénitas [31][32][33][34][35][36][37] , aunque sería necesaria más investigación en humanos para establecer esta asociación, ya que la mayoría de estudios eran ensayos in vitro o con animales.…”

Section: Zincunclassified

“…Determinados autores mostraron que la deficiencia de zinc, aunque es poco frecuente en países desarrollados, podía ocasionar tanto alteraciones en el feto como malformaciones congénitas [31][32][33][34][35][36][37] , aunque sería necesaria más investigación en humanos para establecer esta asociación, ya que la mayoría de estudios eran ensayos in vitro o con animales. Dado que el zinc juega un papel crítico en la síntesis de hemoglobina y en la eritropoyesis, podría desempeñar también un rol significativo en la etiología de la anemia, la cual es indiscutiblemente perjudicial para el embarazo.…”

Section: Zincunclassified

La nutrición en el periodo preconcepcional y los resultados del embarazo: revisión bibliográfica y propuesta de intervención del Dietista-Nutricionista

Molina

Castillo

Jarque

2016

Rev Esp Nutr Hum Diet

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El estado nutricional de la madre antes y durante el embarazo se constata en los últimos años como uno de los factores ambientales más influyentes en el desarrollo fetal. Este estudio se centra en las primeras fases del proceso de gestación o periodo periconcepcional: preconcepción, concepción, implantación, placentación y embriogénesis; puesto que representan un paso crítico en los resultados del embarazo. Tras una revisión bibliográfica exhaustiva de 66 investigaciones publicadas entre los años 1990 y 2015, fueron el zinc, el hierro, el yodo, los ácidos grasos omega-3, el ácido fólico y otras vitaminas del grupo B, los minerales y micronutrientes para los que se han encontrado mayor evidencia de una relación positiva con el desarrollo de la descendencia. Se propone en este estudio el planteamiento de una intervención dietética por parte del Dietista-Nutricionista, tanto individual como colectiva, en el periodo preconcepcional en las mujeres en edad fértil y/o con intenciones de embarazo, pudiéndose continuar dicha intervención en los periodos de gestación, lactancia, postparto e incluso en la descendencia, reportando beneficios a largo plazo y que probablemente se mantengan y manifiesten durante toda la vida del individuo. PALABRAS CLAVE R E S U M E NLa nutrición en el periodo preconcepcional y los resultados del embarazo: revisión bibliográfica y propuesta de intervención del Dietista-Nutricionista

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Effect of increasing zinc sulphate concentration during in vitro maturation of bovine oocytes

Cited by 44 publications

References 45 publications

Zinc Depletion Causes Multiple Defects in Ovarian Function during the Periovulatory Period in Mice

Zinc Depletion Causes Multiple Defects in Ovarian Function during the Periovulatory Period in Mice

Analysis of apoptosis and DNA damage in bovine cumulus cells after exposurein vitroto different zinc concentrations

La nutrición en el periodo preconcepcional y los resultados del embarazo: revisión bibliográfica y propuesta de intervención del Dietista-Nutricionista

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