Effect of increasing dietary concentrate levels on microbial biotin metabolism in the artificial rumen simulation system (RUSITEC)

Abel, Hj.; Immig, Irmgard; Gomez, C. da Costa; Steinberg, W.

doi:10.1080/17450390109386203

Cited by 32 publications

(31 citation statements)

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“…In these experiments, diets contained more than 50% concentrates, compared to an average of 38% in our experiment. The ruminal synthesis of biotin has been reported to be lower in vitro when the pH drops (Abel et al, 2001). In our experiment, we can hypothesize that rumen digestion and biotin synthesis were affected by dietary adaptation during the first weeks of lactation, resulting in a strongly positive effect of biotin supplementation, but that, due to the relatively low amount of concentrate, the ruminal outflow of biotin was sufficient to meet cows requirements after peak lactation even in control cows, resulting in a nonsignificant effect of biotin supplementation.…”

Section: Discussionmentioning

confidence: 99%

Effects of peripartum biotin supplementation of dairy cows on milk production and milk composition with emphasis on fatty acids profile

Enjalbert

Nicot

Packington³

2008

Livestock Science

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Forty Holstein dairy cows receiving a 38% concentrate diet based on maize silage were assigned to either a control group, either a biotin group, receiving 20 mg of biotin per day from 15 days before expected calving date and for 120 days after calving. Milk production was measured daily, milk fat content, protein content, urea and somatic cell counts were determined weekly from week 2 to week 17 of lactation. The profile of milk fatty acids was determined at weeks 3 and 10. Plasma glucose and blood betahydroxybutyrate were determined before calving and at weeks 1, 2, 3, 5, 7 and 10 of lactation.Biotin supplementation resulted in an increased milk production in multiparous cows during weeks 2 to 6, but the effect was no more significant between 7 and 17 weeks of lactation. Milk protein percent was decreased by 0.1% in multiparous cows. Milk fat content was not affected by biotin, and milk fat daily production tended to increase during early lactation. In milk fat, biotin supplementation tended to decrease the proportion of fatty acids with less than 16 carbons at week 3, but the daily amount was not affected. Biotin tended to decrease biohydrogenation intermediates, increased C16:1 at week 3, and tended to increase cis-9 C18:1 at weeks 3 and 10. After 7 weeks of lactation, biotin tended to increase blood beta-hydroxybutyrate in multiparous cows with values remaining in a normal range, and decreased plasma glucose in primiparous cows. These modifications of plasma parameters, milk protein content and profile of milk fatty acids could be due to a higher lipid mobilisation from adipose tissue driven by the increased milk production.

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Section: Discussionmentioning

confidence: 99%

Effects of peripartum biotin supplementation of dairy cows on milk production and milk composition with emphasis on fatty acids profile

Enjalbert

Nicot

Packington³

2008

Livestock Science

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“…Supplementation of biotin at a level of 20 mg/d (equivalent to 1 g/d of 2% product) would cost 2.1 cent of USD. Abel et al (2001) reported that ruminal microbes synthesize more (or degrade less) biotin when the hay replaces barley grain. Biotin synthesis was reduced by 50% when the forage-to-grain ratio decreased from 83:17 to 50:50 (Da Costa Gomez et al, 1998).…”

Section: Feed Intake and Milk Yieldmentioning

confidence: 98%

“…Most of cellulolytic rumen bacteria require biotin for growth (Baldwin and Allison, 1983). Biotin can improve efficient fermentation of fiber in rumen (Abel et al, 2001). Several researchers (Bergsten et al, 1999;Midla et al, 1998;Zimmerly and Weiss, 2001) have reported positive productive and metabolic responses in dairy cattle by supplementation of biotin.…”

Section: Introductionmentioning

confidence: 97%

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Effects of dietary biotin supplementation on performance and hoof quality of Chinese Holstein dairy cows

Chen¹,

Liu²

2012

Livestock Science

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“…Hayes et al (1966) reported similar biotin values in the PFF fraction, although the level of intake for this vitamin was much higher in the present experiment. Abel et al (2001) showed that the substitution of hay for barley in an artificial rumen simulation system decreased biotin levels in the ruminal fractions to a greater extent than in the feed, suggesting that the supply to the ruminant animal is reduced with high-grain diets. The authors concluded that an increase in the proportion of barley in the ration leads either to an increase in destruction or to a reduction in the synthesis of this vitamin.…”

Section: Study 2 Effects Of Forage To Concentrate Ratiomentioning

confidence: 99%

Effects of the forage to concentrate ratio on B-vitamin concentrations in different ruminal fractions of dairy cows

Santschi¹,

Chiquette²,

Berthiaume³

et al. 2005

Can. J. Anim. Sci.

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. 2005. Effects of the forage to concentrate ratio on B-vitamin concentrations in different ruminal fractions of dairy cows. Can. J. Anim. Sci. 85: 389-399. Two studies were undertaken to verify the effect of the forage to concentrate ratio of the diet on B-vitamin concentrations in ruminal contents. In Study 1, eight primiparous and eight multiparous cows were used in a cross-over design, and concentrations of biotin, folates and vitamin B 12 were determined in ruminal fluid and plasma of cows fed a high-forage (HF; 58:42 forage to concentrate ratio; DM basis) or a low-forage (LF; 37:63 forage to concentrate ratio; DM basis) diet. In Study 2, six ruminally cannulated lactating cows were used in a cross-over design to evaluate the effects of forage to concentrate ratio (HF = 60:40; LF = 40:60; DM basis) on concentrations of seven B-vitamins in the particle-free fluid and in both liquid-and solid-associated bacteria. Results showed that B-vitamins were present mainly in the bacterial fractions of the ruminal content, while only limited amounts were found in the surrounding fluid. A change in the forage to concentrate ratio had a greater effect on vitamin concentration in the bacteria associated with the solid fraction than in those present in the liquid portion of the rumen. The most noticeable effects of a low forage diet were an increase in riboflavin, but a decrease in true vitamin B 12 concentrations in solid-associated bacteria as well as a decrease in biotin concentration in particle-free fluid. In conclusion, it appears that ruminal B-vitamin concentration is altered by changes in the forage to concentrate ratio, which suggests that the supply of vitamins to dairy cows is influenced by diet composition. base matière sèche (MS)] ou basse en fourrage (LF; ratio fourrages:concentrés de 37:63; base MS). Dans l'étude 2, 6 vaches en lactation munies de canules ruminales ont été utilisées en chassé-croisé pour évaluer les effets du ratio fourrages:concentrés (HF = 60:40; LF = 40:60; base MS) sur les concentrations de 7 vitamines B dans le fluide sans particules et dans les bactéries associées aux phases liquide et solide. Les résultats démontrent que les vitamines B sont présentes principalement dans les fractions bactériennes du contenu ruminal, et que seulement des quantités limitées sont détectables dans le liquide environnant. Un changement dans le ratio fourrages:concentrés provoque un effet plus marqué sur la concentration des vitamines B dans les bactéries associées à la phase solide que dans celles présentes dans la portion liquide du contenu ruminal. Les effets les plus marqués des rations faibles en fourrages sont une augmentation des concentrations en riboflavine mais une diminution des concentrations en vitamine B 12 dans les bactéries associées à la phase solide ainsi qu'une diminution des concentrations en biotine dans le fluide ruminal sans particules. En conclusion, il semble que la concentration ruminale des vitamines B est altérée par des changements dans le ratio fourrages:concentrés, ce qui su...

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Effect of increasing dietary concentrate levels on microbial biotin metabolism in the artificial rumen simulation system (RUSITEC)

Cited by 32 publications

References 7 publications

Effects of peripartum biotin supplementation of dairy cows on milk production and milk composition with emphasis on fatty acids profile

Effects of peripartum biotin supplementation of dairy cows on milk production and milk composition with emphasis on fatty acids profile

Effects of dietary biotin supplementation on performance and hoof quality of Chinese Holstein dairy cows

Effects of the forage to concentrate ratio on B-vitamin concentrations in different ruminal fractions of dairy cows

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